Journal of Assisted Reproduction and Genetics

, Volume 29, Issue 10, pp 1013–1020 | Cite as

Vitrified blastocysts from Preimplantation Genetic Diagnosis (PGD) as a source for human Embryonic Stem Cell (hESC) derivation

  • Begoña Aran
  • Miquel Sole
  • Ignasi Rodriguez-Pizà
  • Mònica Parriego
  • Yolanda Muñoz
  • Montserrat Boada
  • Pere N. Barri
  • Juan Carlos Izpisúa
  • Anna Veiga
Stem Cell Biology


Embryos diagnosed as abnormal in Preimplantation Genetic Diagnosis (PGD) cycles are useful for the establishment of human Embryonic Stem Cells (hESC) lines with genetic disorders. These lines can be helpful for drug screening and for the development of new treatments. Vitrification has proved to be an efficient method to preserve human blastocysts. One hundred and three abnormal or undiagnosed vitrified blastocysts from the PGD programme at Institut Universitari Dexeus were donated for human embryonic stem cell derivation. The overall survival rate after warming was 70.6 %. Our results showed better survival rates when blastocysts have not started the hatching process (initial/expanded 87.8 %, hatching 68.3 % and hatched 27.3 %). Thirty-five blastocysts and 12 partially surviving embryos were seeded. One hESC line with the multiple exostoses type 2 paternal mutation was obtained.


Blastocyst Vitrification PGD hESC derivation 



The authors want to express their gratitude to Xavier Vendrell from Sistemas Genómicos for his kindly assistance in detection of mutation in the cell line obtained. This work has been done under the auspices of the “Cátedra de Investigación en Obstetrícia y Ginecología de la Universitat Autónoma de Barcelona”.

Conflict of interest

The authors declare that they have not conflict of interest.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Begoña Aran
    • 1
  • Miquel Sole
    • 2
  • Ignasi Rodriguez-Pizà
    • 1
  • Mònica Parriego
    • 2
  • Yolanda Muñoz
    • 1
  • Montserrat Boada
    • 2
  • Pere N. Barri
    • 2
  • Juan Carlos Izpisúa
    • 1
    • 3
  • Anna Veiga
    • 1
    • 2
  1. 1.Stem Cell BankCenter for Regenerative Medicine in BarcelonaBarcelonaSpain
  2. 2.Reproductive Medicine Service, Department of Obstetrics, Gynaecology and Reproductive MedicineInstitut Universitari DexeusBarcelonaSpain
  3. 3.Gene Expression LaboratoryThe Salk Institute for Biological StudiesLa JollaUSA

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