Journal of Assisted Reproduction and Genetics

, Volume 29, Issue 4, pp 353–355 | Cite as

Sperm nucleus decondensation, hyaluronic acid (HA) binding and oocyte activation capacity: different markers of sperm immaturity? Case reports

  • Am Junca
  • Beatriz Gonzalez Marti
  • Elisabetta Tosti
  • Marc Cohen
  • Didier De la fontaine
  • Moncef Benkhalifa
  • Yves MénézoEmail author
Gamete Biology


During the early time of IVF, sperm competence was defined as the ability to fertilize an oocyte. However, with the advent of ICSI, despite the capacity to reach 2–4cell stage, it is impossible for numerous patients, to establish a pregnancy. Instead the consensus now is that male fertility has to be defined as the capacity to produce sperm cells able to establish a full term pregnancy [1, 2, 3] Basic parameters such as concentration, motility and morphology are of limited value in determining sperm capacity to allow full embryonic development to term. Determination of DNA changes like fragmentation and condensation are independent parameters [2] and obviously of major importance. DNA fragmentation, related or not to reactive oxygen species (ROS) insult is only one piece of the problem. Indeed, sperm chromatin tertiary structure seems to be critical for correct epigenetic regulation and maintenance, and further on for male fertility [4, 5]. During very early...


Quercetin Hyaluronic Acid Homocysteine Aniline Blue Sperm Nucleus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    JR Menezo Y. Paternal and maternal factors in preimplantation embryogenesis: interaction with the biochemical environment. RBM Online. 2006;12:616–21.PubMedGoogle Scholar
  2. 2.
    Wyrobek AJ, Eskenazi B, Young S, Arnheim N, Tiemann-Boege I, Jabs EW, Glaser RL, Pearson FS, Evenson D. Advancing age has differential effects on DNA damage, chromatin integrity, gene mutations, and aneuploidies in sperm. Proc Natl Acad Sci USA. 2006;103:9601–6.CrossRefPubMedGoogle Scholar
  3. 3.
    Lewis SE, Aitken RJ. DNA damage to spermatozoa has impacts on fertilization and pregnancy. Cell Tissue Res. 2005;322:33–41.CrossRefPubMedGoogle Scholar
  4. 4.
    Nanassy L, Carrell DT. Abnormal methylation of the promoter of CREM is broadly associated with male factor infertility and poor sperm quality but is improved in sperm selected by density gradient centrifugation. Fertil Steril. 2011;95:2310–4.CrossRefPubMedGoogle Scholar
  5. 5.
    Hammoud SS, Nix DA, Hammoud AO, Gibson M, Cairns BR, Carrell DT. Genome-wide analysis identifies changes in histone retention and epigenetic modifications at developmental and imprinted gene loci in the sperm of infertile men. Hum Reprod. 2011;26:2558–69.CrossRefPubMedGoogle Scholar
  6. 6.
    Zalenskaya IA, Bradbury EM, Zalensky AO. Chromatin structure of telomere domain in human sperm. Biochem Biophys Res Comm. 2000;279:213–8.CrossRefPubMedGoogle Scholar
  7. 7.
    Rousseaux S, Reynoird N, Escoffier E, Thevenon J, Caron C, Khochbin S. Epigenetic reprogramming of the male genome during gametogenesis and in the zygote. Reproductive BioMedicine Online. 2008;16:492–503.CrossRefPubMedGoogle Scholar
  8. 8.
    Puri D, Dhawan J, Mishra RK. The paternal hidden agenda: Epigenetic inheritance through sperm chromatin. Epigenetics. 2010;5:386–91.CrossRefPubMedGoogle Scholar
  9. 9.
    Ward WS. Function of sperm chromatin structural elements in fertilization and development. Mol Hum Reprod. 2010;16:30–6.CrossRefPubMedGoogle Scholar
  10. 10.
    Huszar G, Ozenci CC, Cayli S, Zavaczki Z, Hansch E, Vigue L. Hyaluronic acid binding by human sperm indicates cellular maturity, viability, and unreacted acrosomal status. Fertil Steril. 2003;79 Suppl 3:1616–24.CrossRefPubMedGoogle Scholar
  11. 11.
    Tarozzi N, Nadalini M, Bizzaro D, Serrao L, Fava L, Scaravelli G, Borini A. Sperm-hyaluronan-binding assay: clinical value in conventional IVF under Italian law. Reprod Biomed Online. 2009;19 Suppl 3:35–43.CrossRefPubMedGoogle Scholar
  12. 12.
    Nijs M, Creemers E, Cox A, Janssen M, Vanheusden E, Van der Elst J, Ombelet W. Relationship between hyaluronic acid binding assay and outcome in ART: a pilot study. Andrologia. 2010;42:291–6.CrossRefPubMedGoogle Scholar
  13. 13.
    Morrell JM, Rodriguez-Martinez H. Practical applications of sperm selection techniques as a tool for improving reproductive efficiency. Vet Med Int. 2011, ehead of print.Google Scholar
  14. 14.
    Swann K, Larman MG, Saunders CM, Lai FA. The cytosolic sperm factor that triggers Ca2+ oscillations and egg activation in mammals is a novel phospholipase C: PLCzeta. Reproduction. 2004;127:431–9.CrossRefPubMedGoogle Scholar
  15. 15.
    Tosti E, Ménézo Y. Sperm induced oocyte activation. In: Lejeune T, Delvaux P, editors. Human Spermatozoa: Maturation, Capacitation and Abnormalities. New York: Nova Biomedical Books; Science Publishers Inc; 2010. p. 379–97.Google Scholar
  16. 16.
    Pacheco SE, Houseman EA, Christensen BC, Marsit CJ, Kelsey KT, Sigman M, Boekelheide K. Integrative DNA methylation and gene expression analyses identify DNA packaging and epigenetic regulatory genes associated with low motility sperm. PLoS One. 2011;6:e 20280.CrossRefGoogle Scholar
  17. 17.
    Yamauchi Y, Shaman JA, Ward WS. Non-genetic contributions of the sperm nucleus to embryonic development. Asian J Androl. 2011;13:31–5.CrossRefPubMedGoogle Scholar
  18. 18.
    Ménézo Y, Mares P, Cohen M, Brack M, Viville S, Elder K. Autism, imprinting and epigenetic disorders: a metabolic syndrome linked to anomalies in homocysteine recycling starting in early life?? J Assist Reprod Genet. 2011;28:1143–5.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Am Junca
    • 1
  • Beatriz Gonzalez Marti
    • 1
  • Elisabetta Tosti
    • 2
  • Marc Cohen
    • 3
  • Didier De la fontaine
    • 1
  • Moncef Benkhalifa
    • 1
  • Yves Ménézo
    • 1
    • 3
    • 4
    Email author
  1. 1.UNILABS, Clinique de la MuetteParisFrance
  2. 2.Stazione ZoologicaNaplesItaly
  3. 3.Procrelys, Maison médicale Ambroise ParéLyonFrance
  4. 4.UNILABS Clinique du CotentinParisFrance

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