Sperm transcriptome profiling in oligozoospermia
Investigate in what extent sperm transcriptome of infertile men is different from that of fertile individuals.
Semen samples were collected for determination of sperm parameters as well as for RNA isolation. Gene expression profile was investigated in spermatozoa of 8 infertile and 3 fertile men by microarray analysis using the Affymetrix Chip HG-U133 Plus 2.0.
We observed up to 33-fold reduction expression of genes involved in spermatogenesis and sperm motility. Furthermore, there is an important decrease in expression of genes involved in DNA repair as well as oxidative stress regulation. In this study, we also show a striking drop in expression of histone modification genes.
We found that transcription profile in germ cells of men with idiopathic infertility is different from that of fertile individuals. Interestingly, about 15% of the regulated genes (Eddy Rev Reprod 4:23–30, 1999) play a role in spermatogenesis.
KeywordsSperm Transcripts Oligoasthenoteratozoospermia Male infertility Microarray
- 2.O'Flynn O'Brien KL, Varghese AC, Agarwal A. The genetic causes of male factor infertility: a review. Fertil Steril.;93:1–12.Google Scholar
- 8.Altmae S, Salumets A. A novel genomic diagnostic tool for sperm quality? Reprod Biomed Online.Google Scholar
- 9.Feugang JM, Rodriguez-Osorio N, Kaya A, Wang H, Page G, Ostermeier GC, et al. Transcriptome analysis of bull spermatozoa: implications for male fertility. Reprod Biomed Online.;21:312–24.Google Scholar
- 10.Hamatani T. Spermatozoal RNA profiling towards a clinical evaluation of sperm quality. Reprod Biomed Online.;22:103–5.Google Scholar
- 14.Horowitz E, Zhang Z, Jones BH, Moss SB, Ho C, Wood JR, et al. Patterns of expression of sperm flagellar genes: early expression of genes encoding axonemal proteins during the spermatogenic cycle and shared features of promoters of genes encoding central apparatus proteins. Mol Hum Reprod. 2005;11:307–17.PubMedCrossRefGoogle Scholar
- 19.Liu Z, Zhou S, Liao L, Chen X, Meistrich M, Xu J. Jmjd1a demethylase-regulated histone modification is essential for cAMP-response element modulator-regulated gene expression and spermatogenesis. J Biol Chem.;285:2758–70.Google Scholar
- 26.Garcia-Herrero S, Garrido N, Martinez-Conejero JA, Remohi J, Pellicer A, Meseguer M. Differential transcriptomic profile in spermatozoa achieving pregnancy or not via ICSI. Reprod Biomed Online.;22:25–36.Google Scholar
- 32.Poplinski A, Tuttelmann F, Kanber D, Horsthemke B, Gromoll J. Idiopathic male infertility is strongly associated with aberrant methylation of MEST and IGF2/H19 ICR1. Int J Androl.;33:642–9.Google Scholar