Journal of Assisted Reproduction and Genetics

, Volume 28, Issue 12, pp 1147–1149 | Cite as

Combined strategy for fertility preservation in an oncologic patient: vitrification of in vitro matured oocytes and ovarian tissue freezing

  • Clara GonzálezEmail author
  • Marta Devesa
  • Montserrat Boada
  • Buenaventura Coroleu
  • Anna Veiga
  • Pere N. Barri
Fertility Preservation


Breast cancer is the most common malignancy in women and nearly 7% of cases are diagnosed before age 40 [1]. Diagnosis at early ages is associated with a worse prognosis and therefore, these cases are usually managed with adjuvant chemotherapy and/or hormonal therapy. These treatments are effective in reducing recurrence rates and improving survival rates; however, they have a negative impact on ovarian function and fertility [2]. Fertility preservation in these cases may be of interest also due to the current trend towards delaying motherhood, which leads to a situation where many women may have not fulfilled their motherhood desire at the time of breast cancer diagnosis.

There are several strategies to preserve fertility. Choosing one or the other will depend on patient’s age, tumour biology and, above all, time availability until the start of gonadotoxic treatment.

Case report

We report a case of a 28-year-old patient without relevant past medical history. Menarche...


Ovarian Tissue Ovarian Stimulation Invasive Lobular Carcinoma Antral Follicle Fertility Preservation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was performed under the auspices of “Catedra d’Investigació en Obstetricia, Ginecología i Reproducció” of Department of Obstetrics and Gynaecology, USP-Institut Universitari Dexeus, Universitat Autònoma de Barcelona.


  1. 1.
    Dunn L, Fox KR. Techniques for fertility preservation in patients with breast cancer. Curr Opin Obstet Gynecol. 2009;21:68–73.PubMedCrossRefGoogle Scholar
  2. 2.
    Hickey M, Peate M, Saunders CM, Friedlander M. Breast cancer in young women and its impact on reproductive function. Hum Reprod Update. 2009;15:323–39.PubMedCrossRefGoogle Scholar
  3. 3.
    Kuwayama M. Highly efficient vitrification for cryopreservation of human oocytes and embryos: The Cryo Top method. Theriogenology. 2007;67:73–80.PubMedCrossRefGoogle Scholar
  4. 4.
    Donnez J, Martinez-Madrid B, Jadoul P, Van Langendonckt A, Demylle D, Dolmans MM. Ovarian tissue cryopreservation and transplantation: a review. Hum Reprod Update. 2006;12:519–35.PubMedCrossRefGoogle Scholar
  5. 5.
    Cruz MRS, Prestes JC, Gimenes DL, Fanelli MF. Fertility preservation in women with breast cancer undergoing adjuvant chemotherapy: a systematic review. Fertil Steril. 2010;94:138–43.PubMedCrossRefGoogle Scholar
  6. 6.
    Maltaris T, Weigel M, Mueller A, Schmidt M, Seufert R, Fischl F, et al. Cancer and fertility preservation: fertility preservation in breast cancer patients. Breast Cancer Res. 2008;10:206.PubMedCrossRefGoogle Scholar
  7. 7.
    Oktay K, Buyuk E, Libertella N, Akar M, Rosenwaks Z. Fertility preservation in breast cancer patients: a prospective controlled comparison of ovarian stimulation with tamoxifen and letrozole for embryo cryopreservation. J Clin Oncol. 2005;23:4347–53.PubMedCrossRefGoogle Scholar
  8. 8.
    Demirtas E, Elizur SE, Holzer H, Gidoni Y, Son WY, Chian RC, et al. Immature oocyte retrieval in the luteal phase to preserve fertility in cancer patients. Reprod Biomed Online. 2008;17:520–3.PubMedCrossRefGoogle Scholar
  9. 9.
    Chian RC, Huang JY, Gilbert L, Son WY, Holzer H, Cui SJ, et al. Obstetric outcomes following vitrification of in vitro and in vivo matured oocytes. Fertil Steril. 2009;91:2391–8.PubMedCrossRefGoogle Scholar
  10. 10.
    Smitz JE, Thompson JG, Gilchrist RB. The promise of in vitro maturation in assisted reproduction and fertility preservation. Semin Reprod Med. 2011;29:24–37.PubMedCrossRefGoogle Scholar
  11. 11.
    Oktay K, Buyuk E, Rodriguez-Wallberg KA, Sahin G. In vitro maturation improves oocyte or embryo cryopreservation outcome in breast cancer patients undergoing ovarian stimulation for fertility preservation. Reprod Biomed Online. 2010;20:634–8.PubMedCrossRefGoogle Scholar
  12. 12.
    Rosendahl M, Timmermans Wielenga V, Nedergaard L, Kristensen SG, Ernst E, Rasmussen PE, et al. Cryopreservation of ovarian tissue for fertility preservation: no evidence of malignant cell contamination in ovarian tissue from patients with breast cancer. Fertil Steril. 2011;95:2158–61.PubMedCrossRefGoogle Scholar
  13. 13.
    Schattman GL, Navarro J. Breast cancer and fertility preservation. Placenta. 2008;29:147–51.PubMedCrossRefGoogle Scholar
  14. 14.
    Borini A, Rebellato E. Focus on breast and ovarian cancer. Placenta. 2008;29:184–90.PubMedCrossRefGoogle Scholar
  15. 15.
    Donnez J, Dolmans MM. Preservation of fertility in females with haematological malignancy. Br J Haematol. 2011;154:175–84.PubMedCrossRefGoogle Scholar
  16. 16.
    Donnez J, Kim SS, Albertini DF. Proceedings of the First World Congress on Fertility Preservation: executive summary. J Assist Reprod Genet. 2010;27:191–5.PubMedCrossRefGoogle Scholar
  17. 17.
    Kim SS. Time to re-think: ovarian tissue transplantation versus whole ovary transplantation. Reprod Biomed Online. 2010;20:171–4.PubMedCrossRefGoogle Scholar
  18. 18.
    Telfer EE, McLaughlin M. In vitro development of ovarian follicles. Semin Reprod Med. 2011;29:15–23.PubMedCrossRefGoogle Scholar
  19. 19.
    Huang JY, Tulandi T, Holzer H, Tan SL, Chian RC. Combining ovarian tissue cryobanking with retrieval of immature oocytes followed by in vitro maturation and vitrification: an additional strategy of fertility preservation. Fertil Steril. 2008;89:567–72.PubMedCrossRefGoogle Scholar
  20. 20.
    Huang JY, Tulandi T, Holzer H, Lau NM, Macdonald S, Tan SL, et al. Cryopreservation of ovarian tissue and in vitro matured oocytes in a female with mosaic Turner syndrome: Case Report. Hum Reprod. 2008;23:336–9.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Clara González
    • 1
    Email author
  • Marta Devesa
    • 1
  • Montserrat Boada
    • 1
  • Buenaventura Coroleu
    • 1
  • Anna Veiga
    • 1
    • 2
  • Pere N. Barri
    • 1
  1. 1.Department of Obstetrics, Gynecology and Reproduction, Service of Reproductive MedicineInstitut Universitari DexeusBarcelonaSpain
  2. 2.CMRBCentre for Regenerative Medicine in BarcelonaBarcelonaSpain

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