Frozen-thawed embryo transfer cycles: clinical outcomes of single and double blastocyst transfers

  • Inna Berin
  • Sarah T. McLellan
  • Eric A. Macklin
  • Thomas L. Toth
  • Diane L. Wright
Assisted Reproduction Technologies



To evaluate clinical outcomes of frozen-thawed embryo transfer cycles when one or two blastocysts are transferred.


Retrospective chart review


Two hundred forty-three frozen blastocyst transfer (FBT) cycles were analyzed. Clinical pregnancy rate (50.4% vs. 34.7%), live birth rate (45.8% vs. 30.6%), and twin live birth rate (19.3% vs. 0) were significantly higher in the double versus single FBT group, respectively (p < 0.05). Prior fresh cycle success with same-cohort embryos did not predict outcome of FBT cycle. When the fresh cycle was unsuccessful, there still was a significant increase in twinning when two frozen-thawed blastocysts were transferred.


Transferring two blastocysts during an FBT cycle resulted in higher live birth and twin live birth rates. Single FBT provided acceptable pregnancy rates for couples seeking to avoid a multiple pregnancy or for those having a single blastocyst stored. Interestingly, the outcome of fresh cycle with same-cohort embryos did not influence the outcome of frozen-thawed cycle.


Frozen blastocyst transfer Frozen cycle outcome IVF Multiple pregnancy Number of embryos to transfer 


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Inna Berin
    • 1
  • Sarah T. McLellan
    • 1
  • Eric A. Macklin
    • 2
  • Thomas L. Toth
    • 1
  • Diane L. Wright
    • 1
  1. 1.Vincent Obstetrics and Gynecology Service, Division of Reproductive Medicine and IVFMassachusetts General Hospital, Harvard Medical SchoolBostonUSA
  2. 2.Biostatistics CenterMassachusetts General Hospital, Harvard Medical SchoolBostonUSA

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