The impact of environmental exposure to perfluorinated compounds on oocyte fertilization capacity

  • Laura Governini
  • Raoul Orvieto
  • Cristiana Guerranti
  • Laura Gambera
  • Vincenzo De Leo
  • Paola Piomboni
Assisted Reproduction Technologies


In an attempt to assess the effect of perfluorinated compounds (PFC) on oocytes quality and fertilization rate, we studied follicular fluid (FF) PFC levels in 18 patients undergoing IVF-ET cycles. A significant correlation (R = 0.75; P < 0.001) was observed between FF PFC levels and fertilization rate. Moreover, patients with FF PFC contamination had significantly lower fertilization rate (p < 0.02) and number of embryos transferred (p < 0.02), compared to the PFC negative group.


Infertility IVF PFC contamination Follicular fluid 


  1. 1.
    Sharpe RM. Hormones and testis development and the possible adverse effect of environmental chemicals. Toxicol Lett. 2001;20:221–32.CrossRefGoogle Scholar
  2. 2.
    Taskinen HK, Kyyronen P, Sallmen M, Virtanen SV, Liukkonen TA, Huida O, et al. Reduced fertility among female wood workers exposed to formaldehyde. Am J Ind Med. 1999;36:206–12.PubMedCrossRefGoogle Scholar
  3. 3.
    Law DC, Klebanoff MA, Brock JW, Dunson DB, Longnecker MP. Maternal serum levels of polychlorinated biphenyls and 1, 1-dichloro-2, 2-bis (p-chlorophenyl)ethylene (DDE) and time to pregnancy. Am J Epidemiol. 2005;162:523–32.PubMedCrossRefGoogle Scholar
  4. 4.
    Calafat AM, Wong LY, Kuklenyik Z, Reidy JA, Needham LL. Polyfluoroalkyl chemicals in the U.S. population: data from the National Health and Nutrition Examination Survey (NHANES) 2003–2004 and comparisons with NHANES 1999–2000. Environ Health Perspect. 2007;115:1596–602.PubMedCrossRefGoogle Scholar
  5. 5.
    Renner R. Growing concern over perfluorinated chemicals. Environ Sci Technol. 2001;39:A154–160.CrossRefGoogle Scholar
  6. 6.
    Tittlemier S, Ryan JJ, Oostdam JV. Presence of anionic perfluorinated organic compounds in serum collected from Northern Canadian populations. Organohalogen Compound. 2004;66:4009–14.Google Scholar
  7. 7.
    Giesy JP, Kannan K. Global distribution of perfluorooctane sulfonate in wildlife. Environ Sci Technol. 2001;35:1339–42.PubMedCrossRefGoogle Scholar
  8. 8.
    Midasch O, Drexler H, Hart N, Beckmann MW, Angerer J. Transplacental exposure of neonates to perfluorooctane sulfonate and perfluorooctanoate: a pilot study. Int Arch Occup Environ Health. 2007;80:643–8.PubMedCrossRefGoogle Scholar
  9. 9.
    Fei CY, McLaughlin JK, Tarone RE, Olsen J. Perfluorinated chemicals and fetal growth: a study within the Danish National Birth Cohort. Environ Health Perspect. 2007;115:1677–82.PubMedCrossRefGoogle Scholar
  10. 10.
    Fei C, McLaughlin JK, Lipworth L, Olsen J. Maternal levels of perfluorinated chemicals and subfecundity. Hum Reprod. 2009;24:1200–5.PubMedCrossRefGoogle Scholar
  11. 11.
    Austin ME, Kasturi BS, Barber M, Kannan K, MohanKumar PS, MohanKumar SM. Neuroendocrine effects of perfluorooctane sulfonate in rats. Environ Health Perspect. 2003;111:1485–9.PubMedCrossRefGoogle Scholar
  12. 12.
    Ebner T, Moser M, Sommergruber M, Tews G. Selection based on morphological assessment of oocytes and embryos at different stages of preimplantation development: a review. Hum Reprod Update. 2003;9:251–62.PubMedCrossRefGoogle Scholar
  13. 13.
    Hansen KJ, Clemen LA, Ellefson ME, Johnson HO. Compound-specific, quantitative characterization of organic fluorochemicals in biological matrices. Environ Sci Technol. 2001;35:766–70.PubMedCrossRefGoogle Scholar
  14. 14.
    Kannan K, Hansen SP, Franson CJ, Bowerman WW, Hansen KJ, Jones PD, et al. Perfluorooctane sulfonate in fish-eating water birds including Bald Eagles and Albatrosses. Environ Sci Technol. 2001;35:3065–70.PubMedCrossRefGoogle Scholar
  15. 15.
    Corsolini S, Guerranti C, Perra G, Focardi S. Polybrominated diphenyl ethers, perfluorinated compounds and chlorinated pesticides in Swordfish (Xiphias glaudius) from the Mediterranean Sea. Environ Sci Technol. 2008;42:4344–9.PubMedCrossRefGoogle Scholar
  16. 16.
    Oliva A, Spira A, Multigner L. Contribution of environmental factors to the risk of male infertility. Hum Reprod. 2001;16:1768–76.PubMedCrossRefGoogle Scholar
  17. 17.
    Hauser R, Sokol R. Science linking environmental contaminant exposures with fertility and reproductive health impacts in the adult male. Fertil Steril. 2008;89:e59–65.PubMedCrossRefGoogle Scholar
  18. 18.
    Mendola P, Messer LC, Rappazzo K. Science linking environmental contaminant exposures with fertility and reproductive health impacts in the adult female. Fertil Steril. 2008;89:e81–94.PubMedCrossRefGoogle Scholar
  19. 19.
    Spanò M, Toft G, Hagmar L, Eleuteri P, Rescia M, Rignell-Hydbom A, et al. INUENDO. Exposure to PCB and p, p’-DDE in European and Inuit populations: impact on human sperm chromatin integrity. Hum Reprod. 2005;20:3488–99.PubMedCrossRefGoogle Scholar
  20. 20.
    Giwercman AH, Rignell-Hydbom A, Toft G, Rylander L, Hagmar L, Lindh C, et al. Reproductive hormone levels in men exposed to persistent organohalogen pollutants: a study of inuit and three European cohorts. Environ Health Perspect. 2006;114:1348–53.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Laura Governini
    • 1
    • 2
  • Raoul Orvieto
    • 3
    • 4
  • Cristiana Guerranti
    • 5
  • Laura Gambera
    • 2
    • 6
  • Vincenzo De Leo
    • 2
    • 6
  • Paola Piomboni
    • 1
    • 2
    • 7
  1. 1.Department Biomedical Sciences, Applied Biology SectionUniversity of SienaSienaItaly
  2. 2.Center for Diagnosis and Treatment of Couple SterilityS. Maria alle Scotte HospitalSienaItaly
  3. 3.Infertility and IVF UnitBarzilai Medical CenterAshkelonIsrael
  4. 4.Ben Gurion University of the NegevBeer ShevaIsrael
  5. 5.Department Environmental Sciences “G.Sarfatti”University of SienaSienaItaly
  6. 6.Department Pediatrics, Obstetrics and Reproductive MedicineUniversity of SienaSienaItaly
  7. 7.Department of Biomedical Sciences, Biology Section, Center for the Diagnosis and Treatment of Couple Sterility, S. Maria alle Scotte HospitalUniversity of SienaSienaItaly

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