Sertoli cell-conditioned medium induces germ cell differentiation in human embryonic stem cells

  • Mieke Geens
  • Karen Dora Sermon
  • Hilde Van de Velde
  • Herman Tournaye
Stem Cell Biology

Abstract

Purpose

To investigate the spontaneous germ cell differentiation capacity of VUB hESC lines, develop a protocol for the induction of germ cell differentiation using conditioned medium from Sertoli cells (SCCM) and compare it to existing protocols.

Methods

hESC were allowed to differentiate spontaneously or after the addition of bone morphogenetic proteins (BMPs) and/or SCCM. VASA transcripts were measured by relative quantification real-time RT-PCR to determine the efficiency of germ cell differentiation.

Results

VUB hESC lines can differentiate spontaneously towards the germ cell lineage, however, more consistently in an embryoid body approach than in monolayer cultures. BMPs and SCCM significantly improve VASA expression, but do not have a synergistic effect. Direct contact of differentiating hESC with Sertoli cells does not improve VASA expression.

Conclusions

SCCM contains inductive factors for germ cell differentiation and could represent an element for in-vitro differentiation to germ cells.

Keywords

hESC Sertoli cells Germ cells Differentiation Embryoid bodies 

Notes

Acknowledgements

Our research is supported by grants from the Research Foundation—Flanders (FWO-Vlaanderen) and the research council of the Brussels Free University (OZR).

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Mieke Geens
    • 1
    • 2
  • Karen Dora Sermon
    • 2
  • Hilde Van de Velde
    • 2
    • 3
  • Herman Tournaye
    • 1
    • 3
  1. 1.Research group Biology of the Testis (BITE), Department of Embryology and GeneticsMedical School of the Vrije Universiteit Brussel (VUB)BrusselsBelgium
  2. 2.Research group Reproduction and Genetics (REGE), Department of Embryology and GeneticsMedical School of the Vrije Universiteit Brussel (VUB)BrusselsBelgium
  3. 3.Centre for Reproductive MedicineUniversitair Ziekenhuis Brussel (UZ Brussel)BrusselsBelgium

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