Journal of Assisted Reproduction and Genetics

, Volume 28, Issue 3, pp 253–262 | Cite as

Intracytoplasmic injection of morphologically selected spermatozoa (IMSI) improves outcome after assisted reproduction by deselecting physiologically poor quality spermatozoa

  • Martin Wilding
  • Gianfranco Coppola
  • Loredana di Matteo
  • Antonio Palagiano
  • Enrico Fusco
  • Brian Dale
Assisted Reproduction Technologies

Abstract

Purpose

We used computer assisted sperm selection (MSOME) during cycles of intracytoplasmic sperm injection to test whether this technique improves results over traditional ICSI protocols. We also used the TUNEL assay to test whether MSOME could deselect physiologically abnormal spermatozoa.

Methods

Individual spermatozoa were examined with MSOME. Normal and abnormal spermatozoa were tested for the level of DNA fragmentation using TUNEL assay. In a prospective, randomized trial, patients were selected for standard ICSI, or IMSI techniques. We tested the two groups for biological and clinical parameters.

Results

64.8% of spermatozoa, otherwise selectable for ICSI, were characterized by abnormalities after computer-assisted sperm analysis. These sperm were also characterized by an increase in the level of DNA fragmentation. We noted an increase in embryo quality, pregnancy and implantation rates after computerized sperm selection during ICSI procedures.

Conclusions

Computerised selection of spermatozoa during ICSI procedures deselects physiological abnormal spermatozoa and improves clinical results.

Keywords

MSOME IMSI TUNEL assay ICSI Human embryo 

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Martin Wilding
    • 1
  • Gianfranco Coppola
    • 1
  • Loredana di Matteo
    • 1
    • 2
  • Antonio Palagiano
    • 1
  • Enrico Fusco
    • 1
  • Brian Dale
    • 1
  1. 1.Centro Fecondazione AssistitaClinica Villa del SoleNapoliItaly
  2. 2.Facolta di Medicina e Chirurgia, II Università degli Studi di NapoliNapoliItaly

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