Successful live birth from vitrified oocytes after 5 years of cryopreservation
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To report a birth of a healthy boy after long-term cryopreservation of oocytes by vitrification.
A 17 year-old female with secondary pulmonary hypertension caused by transposition of great vessels visited our center in 2002, and she wished oocytes cryopreservation to avoid possible sterility after the following category X medication treatment.
Vitrified oocytes on Electron Microscope (EM) grids were warmed after 5 years of storage. Surviving MII oocytes were microinjected for fertilization and two embryos were transferred into a gestational carrier day 5 after microinjection.
Main Outcome Measure(s)
Survival, fertilization, cleavage, clinical pregnancy and delivery.
Eleven out of fourteen oocytes (78.6%) survived warming. Eight Metaphase II (MII) oocytes and 3 in vitro matured oocytes were microinjected; all 11 oocytes (100%) fertilized and 2 embryos were transferred on day 5. A healthy baby boy weighing 3,600 g was delivered at 38 weeks of gestation. Live-birth rates per warmed oocyte and per injected oocyte were 7.1% and 9.1% respectively.
Cryopreservation after vitrification with EM grids maintained the developmental competence of oocytes after long-term storage and resulted in a successful live birth.
KeywordsHuman oocyte cryopreservation Vitrification Electron microscope grid Ethylene glycol Clinical outcome
We would like to thank Professor Rogerio Lobo, M.D. (Department of Obstetrics and Gynecology, Columbia University College of Physicians and Surgeons, New York, NY) for his valuable suggestions during the preparation of this manuscript.