In vitro maturation of oocytes via the pre-fabricated self-assembled artificial human ovary
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Create a 3-Dimensional artificial human ovary to mature human oocytes.
Theca and granulosa cells were isolated from antral follicles of reproductive-aged women, seeded into micro-molded gels and self-assembled into complex 3D microtissues. Immunohistochemistry and live-dead staining confirmed theca cell identity and cellular viability at one week respectively. Placement of granulosa cell spheroids or cumulus-oocyte complexes into theca cell honeycomb openings resulted in creation of an artificial human ovary. Oocytes from this construct were assessed for polar body extrusion.
Theca and granulosa cells self-assembled into complex microtissues, remaining viable for one week. At 72 h after artificial human ovary construction, theca cells completely surrounded the granulosa spheroids or COCs without stromal invasion or disruption. Polar body extrusion occurred in one of three COCs assessed.
An artifical human ovary can be created with self-assembled human theca and granulosa cell microtissues, and used for IVM and future oocyte toxicology studies.
KeywordsArtificial ovary In vitro maturation Oocytes Self-assembly Theca cells Tissue engineering
This research was supported by Research Funds from the Division of Reproductive Endocrinology & Infertility at the Women & Infants’ Hospital of Rhode Island, and by a grant from the Rhode Island Science and Technology Council.
- 3.Silber S, Kagawa N, Kuwayama M, Gosden R. Duration of fertility after fresh and frozen ovary transplantation. Fertil Steril 2010 Feb 18. [Epub ahead of print]Google Scholar
- 19.Gougeon A. Dynamics of follicular growth in the human: a model from preliminary results. Human Reprod. 1986;1:81–7.Google Scholar
- 25.Spears N, Boland NI, Murray AA, Gosden RG. Mouse oocytes derived from in vitro grown primary ovarian follicles are fertile. Human Reprod. 1994;9:527–32.Google Scholar
- 26.Cortvrindt R, Smitz J, Van Steirteghem AC. In vitro maturation, fertilization and embryo development of immature oocytes from early preantral follicles from prepubertal mice in a simplified culture system. Human Reprod. 1996;11:2656–6.Google Scholar