Journal of Assisted Reproduction and Genetics

, Volume 27, Issue 9–10, pp 533–538 | Cite as

Seminal plasma homocysteine, folate and cobalamin in men with obstructive and non-obstructive azoospermia

  • Igor CrhaEmail author
  • Michaela Kralikova
  • Jitka Melounova
  • Pavel Ventruba
  • Jana Zakova
  • Rastislav Beharka
  • Richard Husicka
  • Michal Pohanka
  • Martin Huser
Gonadal Physiology and Disease



The aim of this study was to analyze homocysteine, folate and cobalamin in men with normozoospermia, obstructive and non-obstructive azoospermia.


Analysis of plasma and seminal plasma homocysteine, folate and cobalamin in 72 azoospermic and 62 normozoospermic men. Evaluation of the azoospermic patient included testicular biopsy, endocrine, urological and ultrasound examination.


Homocysteine (1.2 μmol/l) and cobalamin (322.05 pmol/l) concentrations (median values) in seminal plasma were significantly lower (p < 0.001) in men with azoospermia than in men with normozoospermia (2.5 μmol/l and 579.0 pmol/l). Folate and cobalamin concentrations were significantly higher in obstructive than in non-obstructive azoospermia. Significant correlations were determined between testis volume and seminal plasma homocysteine in azoospermic men.


Lower concentrations of homocysteine and cobalamin (but not folate) were found in azoospermic seminal plasma than normozoospermic. Folate and cobalamin were higher in seminal plasma from obstructive azoospermia than in non-obstructive azoospermia patients.


Azoospermia Cobalamin Folate Homocysteine Spermatogenesis 



This work was supported by Internal Grant Agency (IGA) of the Ministry of Health of the Czech Republic-No. NS/9661.


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Igor Crha
    • 1
    Email author
  • Michaela Kralikova
    • 2
  • Jitka Melounova
    • 2
  • Pavel Ventruba
    • 1
  • Jana Zakova
    • 1
  • Rastislav Beharka
    • 3
    • 4
  • Richard Husicka
    • 1
  • Michal Pohanka
    • 5
  • Martin Huser
    • 1
  1. 1.Department of Gynecology and Obstetrics, Faculty of MedicineMasaryk University, and Faculty HospitalBrnoCzech Republic
  2. 2.Department of Biochemistry, Faculty of MedicineMasaryk UniversityBrnoCzech Republic
  3. 3.Department of Urology, Faculty of MedicineMasaryk University and Faculty Hospital BrnoBrnoCzech Republic
  4. 4.Department of Medical GeneticsFaculty Hospital BrnoBrnoCzech Republic
  5. 5.Department of Functional Diagnostics and RehabilitationSt. Anne’s Faculty Hospital BrnoBrnoCzech Republic

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