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Journal of Assisted Reproduction and Genetics

, Volume 27, Issue 8, pp 469–476 | Cite as

Lithium protects against toxic effects of cadmium in the rat testes

  • Majedah Al-Azemi
  • Florence E. Omu
  • Elijah O. Kehinde
  • Jeroham T. Anim
  • Mabayoje A. Oriowo
  • Alexander E. Omu
Gonadal Physiology and Disease

Abstract

Purpose

To investigate the protective effect of Lithium against the toxic effect of Cadmium in the rat testes.

Methods

Twenty four adult male Sprague-Dawley rats were treated with four different regimens: Cadmium only, Cadmium and lithium, lithium only and controls. Rats were sacrificed after 6 weeks and testicular levels of pro-inflammatory cytokine (IL-4), anti-inflammatory cytokine (TNF-α), Pro-apoptotic protein (Bax) and anti-apoptotic protein (Bcl-2) were measured by ELISA while serum levels of FSH, LH, Prolactin and Testosterone were measured using the Vidas parametric system. Antioxidant status (MDA, SOD) was also assessed in serum. Histopathological changes of testes were examined using light and electron microscopy. Immunohistochemical staining for Bax, Bcl-2 and Caspase 3 were performed.

Results

Treatment with lithium was associated with significant reduction in the toxic effects of Cadmium as shown by reduced testicular levels of TNF-α, serum levels of Malondialdehyde and testicular level of Bax, and increased levels of IL-4, Zn-Cu SOD, Bcl-2 and Testosterone. Testicular histopathology showed that Cadmium produced an extensive germ cells apoptosis and the addition of lithium in Cadmium-treated rats significantly reduced cadmium-induced testicular damage.

Conclusion(s)

Lithium has a protective effect against cadmium-induced testicular apoptosis in the rat.

Keywords

Apoptosis Bax Bcl-2 Lithium Testes Cadmium 

Notes

Disclosure information

All authors have nothing to declare.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Majedah Al-Azemi
    • 1
  • Florence E. Omu
    • 2
  • Elijah O. Kehinde
    • 3
  • Jeroham T. Anim
    • 4
  • Mabayoje A. Oriowo
    • 5
  • Alexander E. Omu
    • 1
  1. 1.Department of Obstetrics and Gynaecology, Faculty of MedicineKuwait UniversitySafatKuwait
  2. 2.College of NursingPublic Authority for Applied Education and Training (PAAET)SafatKuwait
  3. 3.Department of Surgery, Faculty of MedicineKuwait UniversitySafatKuwait
  4. 4.Department of Pathology, Faculty of MedicineKuwait UniversitySafatKuwait
  5. 5.Department of Pharmacology and Toxicology, Faculty of MedicineKuwait UniversitySafatKuwait

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