Evaluation of the developmental competence and chromosomal compliment of mouse oocytes derived from in-vitro growth and maturation of preantral follicles

Animal Experimentation

Abstract

Purpose

To evaluate the developmental potential and aneuploidy rates of in-vitro versus in-vivo grown and matured mouse oocytes.

Methods

Mice were superovulated to obtain in-vivo matured oocytes. Mouse preantral follicles were also mechanically isolated and cultured in-vitro. In-vitro fertilization (IVF) was performed and fertilization, cleavage, and morula/blastocyst formation rates were compared between groups. Cytogenetic analysis was used to compare oocyte aneuploidy rates and aneuploidy characteristics in the developing embryos.

Results

In-vivo oocyte maturation resulted in higher IVF fertilization, cleavage, and morula/blastocyst formation rates versus in-vitro follicle culture (96.4% versus 78.5%, p < 0.001; 95.3% versus 77.4%, p < 0.001; 94.1% versus 76.9%, p < 0.001). Total aneuploidy rates were higher in embryos derived from in-vitro matured oocytes versus those grown in-vivo (4.0% versus 1.3%, p < 0.05).

Conclusions

Results indicate a reduced developmental competency of in-vitro matured oocytes. The data also highlight an increased susceptibility to meiotic errors in early stage follicles undergoing in vitro culture.

Keywords

Aneuploidy Follicle In-vitro Maturation Mosaicism Oocyte 

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Lihua Liu
    • 1
    • 2
  • Vincent W. Aoki
    • 1
    • 2
  • Douglas T. Carrell
    • 1
    • 2
    • 3
    • 4
  1. 1.Andrology and IVF LaboratoriesUniversity of Utah School of MedicineSalt Lake CityUSA
  2. 2.Department of Surgery (Urology)University of Utah School of MedicineSalt Lake CityUSA
  3. 3.Department of PhysiologyUniversity of Utah School of MedicineSalt Lake CityUSA
  4. 4.Department of Obstetrics and GynecologyUniversity of Utah School of MedicineSalt Lake CityUSA

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