Advertisement

Comparison of c-DNA microarray analysis of gene expression between eutopic endometrium and ectopic endometrium (endometriosis)

  • L. Mettler
  • A. Salmassi
  • T. Schollmeyer
  • A. G. Schmutzler
  • F. Püngel
  • W. Jonat
Original Paper

Abstract

Problem: As recent studies have suggested abnormalities in the regulation of specific genes in the development of endometriosis, we investigated differentially expressed genes in endometriosis compared to endometrium. Method of study: Gene expression profiles using the Atlas microarray were performed in endometriotic tissue and endometrium. Nine of the 13 genes of endometriotic tissue showed an up-regulation in relation to endometrium and four of the 13 genes a down-regulation. Results: Of the 1176 genes on the Atlas Human 1,2 array, only 13 differentially expressed identical genes were detected after repeating the gene analysis three times. Conclusion: According to our c-DNA analysis some differentially expressed genes may be involved in the pathogenesis of endometriosis. An imbalance in the genes responsible for the reproductive process may lead to a decrease in embryo implantation in patients with endometriosis.

Keywords

Endometriosis Endometrium Microarray analysis 

References

  1. 1.
    Schenken RS. Endometriosis. In: Scott JR, Di Sain PJ, Hammond CB, Spellacy WN, (eds). Danforth's Obstetrics and Gynecology. 8th edn. Baltimore, Lippincott: Williams and Wilkins; 1999. p. 669–75Google Scholar
  2. 2.
    Speroff L, Glass RH, Käse NG. Endometriosis. In: Speroff L, Glass RH, Käse NG, (eds). Clinical gynecologic endocrinology and infertility. 6th edn. Baltimore, Lippincott: Williams and Wilkins; 1999. p. 1057–73Google Scholar
  3. 3.
    Ota H, Igarashi S, Kato N, Tanaka T. Aberrant expression of glutathi-one peroxidase in eutopic and ectopic endometrium in endometriosis and adenomyosis. Fertil Steril 2000;74:313–8PubMedCrossRefGoogle Scholar
  4. 4.
    Sharpe-Timms K, Piva M, Ricke EA, Surewicz K, Zhang YL, Zimmer RL. Endometriotic lesions synthesize and secrete a haptoglobin-like protein. Biol Reprod 1998;58:988–94PubMedCrossRefGoogle Scholar
  5. 5.
    Tsudo T, Harada T, Iwabe T, Tanikawa M, Nagano Y, Ito M, Taniguchi F, Terakawa N. Altered gene expression and secretion of interleukin-6 in stromal cells derived from endometriotic tissues. Fertil Steril 2000;73:205–11PubMedCrossRefGoogle Scholar
  6. 6.
    Kao LC, Germeyer A, Tulac S, Lobo S, Yang JP, Taylor RN, Osteen K, Lessey BA, Giudice LC. Expression profiling of endometrium from women with endometriosis reveals candidate genes for disease-based implantation failure and infertility. Endocrinology 2003;144:2870–81PubMedCrossRefGoogle Scholar
  7. 7.
    Matsuzaki S, Canis M, Vaurs-Barriere C, Boespflug-Tanguy O, Dastugue B, Mage G. DNA microarray analysis of gene expression in eutopic endometrium from patients with deep endometriosis using laser capture microdissection. Fertil Steril 2005;84 Suppl 2:1180–90PubMedCrossRefGoogle Scholar
  8. 8.
    Wu Y, Kajdacsy-Balla A, Strawn E, Basir Z, Halverson G, Jailwala P, Wang Y, Wang X, Ghosh S, Guo SW. Transcriptional characterizations of differences between eutopic and ectopic endometrium. Endocrinology. 2006;147(1):232–46. Epub 2005 Sep 29.PubMedCrossRefGoogle Scholar
  9. 9.
    Hu WP, Tay SK, Zhao Y. Endometriosis-specific genes identified by real-time reverse transcription-polymerase chain reaction expression profiling of endometriosis versus autologous uterine endometrium. J Clin Endocrinol Metab 2006;91(1):228–38. Epub 2005 Oct 25PubMedCrossRefGoogle Scholar
  10. 10.
    Zeitoun K, Takayama K, Sasano H, Suzuki T, Moghrabi N, Andersson S, Johns A, Meng L, Putman M, Carr B et al. Deficient 17β-hydroxysteroid dehydrogenase type 2 expression in endometriosis: failure to metabolize 17β-estradiol. J Clin Endocrinol Metab 1998;83:4474–80PubMedCrossRefGoogle Scholar
  11. 11.
    Bruner-Tran KL, Eisenberg E, Yeaman GR, Anderson TA, McBean J, Osteen KG. Steroid and cytokine regulation of matrix metalloproteinase expression in endometriosis and the establishment of experimental endometriosis in nude mice. J Clin Endocrinol Metab 2002;87:4782–91PubMedCrossRefGoogle Scholar
  12. 12.
    Nagasawa A, Kubota R, Imamura Y, Nagamine K, Wang Y, Asakawa S, Kudoh J, Minoshima S, Mashima Y, Oguchi Y, Shimizu N. Cloning of the cDNA for a new member of the immunoglobulin superfamily (ISLR) containing leucine-rich repeat (LRR). Genomics 1997;44:273–9PubMedCrossRefGoogle Scholar
  13. 13.
    Xu H, Di W, Chen D. Expression of human leucocyte antigen in ectopic endometrial tissue of endometriosis. Zhonghua Fu Chan Ke Za Zhi 2001;36:473–5PubMedGoogle Scholar
  14. 14.
    Van Langendonckt A, Casanas-Roux F, Donnez J. Iron overload in the peritoneal cavity of women with pelvic endometriosis. Fertil Steril 2002;78:712–8PubMedCrossRefGoogle Scholar
  15. 15.
    Ethier JF, Lussier JG, Silversides DW. Bovine activin receptor type IIB messenger ribonucleic acid displays alternative splicing involving a sequence homologous to Src-homology 3 domain binding sites. Endocrinology 1997;138:2425–34PubMedCrossRefGoogle Scholar
  16. 16.
    Loddenkemper C, Mechsner S, Foss HD, Dallenbach FE, Anagnostopoulos I, Ebert AD, Stein H. Use of oxytocin receptor expression in distinguishing between uterine smooth muscle tumors and endometrial stromal sarcoma. Am J Surg Pathol 2003;27:1458–62PubMedGoogle Scholar
  17. 17.
    Metaxa-Mariatou V, McGavigan CJ, Robertson K, Stewart C, Cameron IT, Campbell S. Elastin distribution in the myometrial and vascular smooth muscle of the human uterus. Mol Hum Reprod 2002;8:559–65PubMedCrossRefGoogle Scholar
  18. 18.
    Suske G, Mengel T, Cordingley M, Kadenbach B. Molecular cloning and further characterization of cDNAs for rat nuclear-encoded cytochrome c oxidase subunits VIc and VIII. Eur J Biochem 1987;168:233–7PubMedCrossRefGoogle Scholar
  19. 19.
    Foti D, Stroup D, Chiang JY. Basic transcription element binding protein (BTEB) transactivates the cholesterol 7 alpha-hydroxylase gene (CYP7A). Biochem Biophys Res Commun 1998;253:109–13PubMedCrossRefGoogle Scholar
  20. 20.
    Bulun SE, Fang Z, Imir G, Gurates B, Tamura M, Yilmaz B, Langoi D, Amin S, Yang S, Deb S. Aromatase and endometriosis. Semin Reprod Med 2004;22:45–50PubMedCrossRefGoogle Scholar
  21. 21.
    Eyster KM, Boles AL, Brannian JD, Hansen KA. DNA microarray analysis of gene expression markers of endometriosis. Fertil Steril 2002;77:38–42PubMedCrossRefGoogle Scholar
  22. 22.
    Burns PD, Hayes SH, Silvia WJ. Cellular mechanisms by which oxytocin mediates uterine prostaglandin F2 alpha synthesis in bovine endometrium: role of calcium. Domest Anim Endocrinol 1998;15:477–87PubMedCrossRefGoogle Scholar
  23. 23.
    Riley SC, Poyser NL. Prostaglandin production by the guinea-pig endometrium: is calcium necessary? J Endocrinol 1987;113:463–71PubMedCrossRefGoogle Scholar
  24. 24.
    Koike H, Ikenoue T, Mori N. Studies on prostaglandin production relating to the mechanism of dysmenorrhea in endometriosis. Nippon Naibunpi Gakkai Zasshi 1994;70:43–56PubMedGoogle Scholar
  25. 25.
    Higuchi T, Kanzaki H, Fujimoto M, Hatayama H, Watanabe H, Fukumoto M, Kaneko Y, Higashitsuji H, Kishishita M, Mori T et al. Expression of vav proto-oncogene by non hematopoietic trophoblast cells at the human uteroplacental interface. Biol Reprod 1995;53:840–6PubMedCrossRefGoogle Scholar
  26. 26.
    Srodon M, Klein WM, Kurman RJ. CD10 imunostaining does not distinguish endometrial carcinoma invading myometrium from carcinoma involving adenomyosis. Am J Surg Pathol 2003;27:786–9PubMedCrossRefGoogle Scholar
  27. 27.
    Hoyt MA. Cellular roles of kinesin and related proteins. Curr Opin Cell Biol 1994;6:63–8PubMedCrossRefGoogle Scholar
  28. 28.
    Vallee RB, Sheetz MP. Targeting of motor proteins. Science 1996;271:1539–44PubMedCrossRefGoogle Scholar
  29. 29.
    Davidson WF, Kress M, Khoury G, Jay G. Comparison of HLA class I gene sequences. Derivation of locus-specific oligonucleotide probes specific for HLA-A, HLA-B, and HLA-C genes. J Biol Chem 1985;260:13414–23PubMedGoogle Scholar
  30. 30.
    Roblero LS, Guadarrama A, Ortiz ME, Fernandez E, Zegers-Hochschild F. High potassium concentration and the cumulus corona oocyte complex stimulate the fertilizing capacity of human spermatozoa. Fertil Steril 1990;54:328–32PubMedGoogle Scholar
  31. 31.
    Roblero LS, Riffo MD. High potassium concentration improves preimplantation development of mouse embryos in vitro. Fertil Steril 1986;45:412–6PubMedGoogle Scholar
  32. 32.
    Levin RJ, Edwards F. The transuterine endometrial potential difference, its variation during the oestrous cycle and its relation to uterine secretion. Life Sci 1968;7:1019–36PubMedCrossRefGoogle Scholar
  33. 33.
    Sun XC, Zhai CB, Cui M, Chen Y, Levin LR, Buck J, Bonanno JA. HCO(3)(-)-dependent soluble adenylyl cyclase activates cystic fibrosis transmembrane conductance regulator in corneal endothelium. Am J Physiol Cell Physiol 2003;284:C1114–22PubMedGoogle Scholar
  34. 34.
    Lenka N, Vijayasarathy C, Mullick J, Avadhani NG. Structural organization and transcription regulation of nuclear genes encoding the mammalian cytochrome c oxidase complex. Prog Nucleic Acid Res Mol Biol 1998;61:309–44PubMedCrossRefGoogle Scholar
  35. 35.
    Patterson A, Karsi A, Feng J, Liu Z. Translational machinery of channel catfish: II. Complementary DNA and expression of the complete set of 47 60S ribosomal proteins. Gene 2003;305:151–60PubMedCrossRefGoogle Scholar
  36. 36.
    Zhao Y, Sohn JH, Warner JR. Autoregulation in the biosynthesis of ribosomes. Mol Cell Biol 2003;23:699–707PubMedCrossRefGoogle Scholar
  37. 37.
    Chang SY, Park SG, Kim S, Kang CY. Interaction of the C-terminal domain of p43 and the alpha subunit of ATP synthase. Its functional implication in endothelial cell proliferation. J Biol Chem 2002;277:8388–94PubMedCrossRefGoogle Scholar
  38. 38.
    Wells J, Henkler F, Leversha M, Koshy RA. Mitochondrial elongation factor-like protein is over-expressed in tumours and differentially expressed in normal tissues. FEBS Lett 1995;358:119–25PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • L. Mettler
    • 1
  • A. Salmassi
    • 1
  • T. Schollmeyer
    • 1
  • A. G. Schmutzler
    • 1
  • F. Püngel
    • 1
  • W. Jonat
    • 1
  1. 1.Department of Obstetrics & GynaecologyUniversity Hospitals Schleswig-HolsteinKielGermany

Personalised recommendations