Effect of micro-environment maintenance on embryo culture after in-vitro fertilization: comparison of top-load mini incubator and conventional front-load incubator

  • Mutsuko Fujiwara
  • Kentaro Takahashi
  • Mika Izuno
  • Ya Ru Duan
  • Maya Kazono
  • Fuminori Kimura
  • Yoichi Noda
Assisted Reproduction

Abstract

Purpose: To investigate the effect of microenvironment maintenance on embryo culture and clinical results using two types of incubators.

Methods: Temperature and oxygen concentration in a mini-incubator and a conventional incubator were compared following a 5-s door opening/closing procedure. Embryos of 30 in-vitro fertilization embryo transfer (IVF-ET) cases were randomly allocated to either one of the incubator, cultured, and the early-stage good embryo formation rate and the good blastocyst formation rate were compared, as indicators for micro-environment maintenance ability.

Results: Temperature recovery after a 5-s door opening/closing procedure was approximately 5 min for the mini-incubator and 30 min for the conventional incubator. The oxygen concentration return was significantly improved in the mini-incubator (3.0 ± 0 min) compared with the conventional incubator (7.8 ± 0.9 min). Both the early-stage good embryo formation rate and the good blastocyst formation rate were significantly higher in the mini-incubator (39.5% and 15.1%) than the conventional incubator (28.4% and 7.8%).

Conclusion: The microenvironment maintenance ability of incubators appears to significantly influence the formation of good embryos.

Keywords

IVF-ET Incubator Micro-environment maintenance Embryo formation rate 

 

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Copyright information

© Springer Science+Business Media, LLC 2006

Authors and Affiliations

  • Mutsuko Fujiwara
    • 1
  • Kentaro Takahashi
    • 1
  • Mika Izuno
    • 1
  • Ya Ru Duan
    • 1
  • Maya Kazono
    • 1
  • Fuminori Kimura
    • 1
  • Yoichi Noda
    • 1
  1. 1.Department of Obstetrics and GynecologyShiga University of Medical Science, Seta-Tsukinowa-choOhtsuJapan

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