Journal of Assisted Reproduction and Genetics

, Volume 24, Issue 1, pp 37–41

Discordance among blastomeres renders preimplantation genetic diagnosis for aneuploidy ineffective

  • C. B. Coulam
  • R. S. Jeyendran
  • M. Fiddler
  • E. Pergament
Original paper

DOI: 10.1007/s10815-006-9073-x

Cite this article as:
Coulam, C.B., Jeyendran, R.S., Fiddler, M. et al. J Assist Reprod Genet (2007) 24: 37. doi:10.1007/s10815-006-9073-x

Abstract

Purpose: To investigate the contribution of discordance among blastomeres from the same embryo in the interpretation of blastomeres biopsied from day 3 embryos.

Methods: 228 IVF embryos had two blastomeres removed and fluorescent in situ hybridization (FISH) was used to detect aneuploidy of chromosomes 13, 15, 16, 18, 21, 22, X and Y. Of the 228 embryos, 102 had complete FISH results for both blastomeres.

Results: When the 2 blastomeres of 102 embryos with successful FISH results were compared, 26 (25.5%) were concordant for all 8 chromosomes and 76 (74.5%) were discordant for one or more chromosomes. Among the 102 embryos, 12 (12%) were disomy in both blastomeres and 37 (36%) were disomic in all 8 chromosomes in one of the two blastomeres.

Conclusion: Discordance among blastomeres from the same embryo appears to present a significant problem in interpreting results of embryos biopsied on day 3 and analyzed by FISH especially when most PGD’s are done on single blastomeres.

Keywords

Preimplantation genetic diagnosis Aneuploidy screening Discordance among blastomeres Chromosomal discordance 

Copyright information

© Springer Science+Business Media, LLC 2006

Authors and Affiliations

  • C. B. Coulam
    • 1
  • R. S. Jeyendran
    • 2
  • M. Fiddler
    • 3
  • E. Pergament
    • 4
  1. 1.Pregnancy Success Center of the Rinehart Center for Reproductive MedicineChicagoUSA
  2. 2.Andrology Laboratory ServicesChicagoUSA
  3. 3.DePaul UniversityChicagoUSA
  4. 4.Northwestern Reproductive GeneticsChicagoUSA

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