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Journal of Assisted Reproduction and Genetics

, Volume 21, Issue 12, pp 445–451 | Cite as

Relationship Between Standard Semen Parameters, Calcium, Cholesterol Contents, and Mitochondrial Activity in Ejaculated Spermatozoa From Fertile and Infertile Males

  • Marcos Meseguer
  • Nicolás Garrido
  • Jose Antonio Martínez-Conejero
  • Carlos Simón
  • Antonio Pellicer
  • Jose Remohí
Andrology

Abstract

Purpose: To correlate levels of cholesterol (CH), calcium (Ca2+), and mitochondrial activity (MA) with the standard semen parameters and to compare them between fertile and infertile men.

Methods: We studied 151 semen samples from infertile (n=60) or fertile (n=91) males. Basic sperm parameters were analyzed. Ca2+ and CH concentrations on seminal plasma were determined by enzymoimmunoanalysis. Intracellular Ca2+ and CH concentrations in the sperm plasma membrane and mitochondrial activity by fluorometry.

Results: There was a significant positive correlation between sperm membrane CH and sperm morphology. Intracellular Ca2+ was lower in infertile patients compared to fertile. No differences were found regarding Ca2+ and CH concentrations in seminal plasma. MA is directly and strongly related with sperm motility.

Conclusions: Intracellular concentrations of Ca2+ and the proportion of CH in the sperm membrane are two important markers of the sperm quality due to its direct relationship with sperm morphology and fertility potential.

Ca2+ cholesterol fertility mitochondrial activity human spermatozoa 

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Copyright information

© Springer Science+Business Media, Inc. 2004

Authors and Affiliations

  • Marcos Meseguer
    • 1
    • 2
  • Nicolás Garrido
    • 1
    • 2
  • Jose Antonio Martínez-Conejero
    • 1
    • 2
  • Carlos Simón
    • 1
    • 3
  • Antonio Pellicer
    • 1
    • 3
    • 4
  • Jose Remohí
    • 1
    • 3
  1. 1.IVI ValenciaSpain
  2. 2.Fundación IVISpain
  3. 3.Department of Pediatrics, Obstetrics and GynecologyValencia University School of MedicineSpain
  4. 4.Department of Obstetrics and Gynecology Hospital Dr. PesetSpain

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