Journal of Applied Spectroscopy

, Volume 76, Issue 6, pp 864–875 | Cite as

Analysis of metal ion concentration in humic acid by excitation-emission matrix fluorescence and chemometric methods

  • O. Divya
  • V. Venkataraman
  • A. K. Mishra

In this study, using a representative system of a humic acid sample (Aldrich), a method for the quantification of the interaction of metal ions (Cu(II), Pb(II), Cd(II), Zn(II)) with HA has been developed by combining EEMF and chemometrics. N-way partial least squares regression (N-PLS) and parallel factor analysis (PARAFAC) have been effectively applied to the fluorescence data of HA samples having varying metal ion concentrations. The accuracy of the method, as evaluated from the root mean square error of prediction (RMSEP) value, is found to be very good in the case of Cu and Pb.


humic acid excitation-emission matrix fluorescence spectroscopy parallel factor analysis N-way partial least squares regression chemometric analysis 


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Copyright information

© Springer Science+Business Media, Inc. 2009

Authors and Affiliations

  1. 1.Department of ChemistryIndian Institute of Technology MadrasChennai-36India
  2. 2.CEERI Centre, CSIR Complex TaramaniChennaiIndia

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