Surface-to-volume ratio influence on the growth of Scenedesmus obliquus in a thin-layer cascade system
Microalgae biomass is a source of a wide range of high commercial value compounds such as proteins, carotenoids, lipids and polysaccharides that have potential applications in several biotechnological processes. This study evaluated the influence of two different illuminated surface-to-volume-ratios (S/V) on the growth in cultivation of Scenedesmus obliquus in a thin-layer cascade system (TLC). Two S/V ratios (80 m−1 and 60 m−1) were used, corresponding to water columns of 0.5 cm and 1.0 cm, respectively. The cultures were compared in terms of the maximum biomass attained, volumetric and area productivities, photosynthetic efficiency, and CO2 fixation into biomass. The maximum biomass achieved was 20.14 g L−1 in SV80 cultures while the SV60 treatments attained 14.60 g L−1, with a maximum volumetric productivity of 2.42 and 1.85 g L−1 day−1, respectively. The culture with lower S/V was more efficient in relation to CO2 fixation, reaching 62% of the total carbon offered in comparison to the higher S/V (54%). Concerning the incident energy in the daylight period, both treatments presented similar photosynthetic efficiency values.
KeywordsIntensive culture Thin-layer cascade system Productivity Biomass Chlorophyta
H.C.V and H.C. built and set up the TLC and conducted the data acquisition and analysis. H.C.V, R.G.L, and R.B.D developed the experimental design, conducted the data interpretation, and wrote this manuscript.
This study received financial support from the Brazilian Ministry of Science, Technology, Innovation, and Communications–MCTIC/CGTS/SETEC provided by the Funding Authority for Studies and Projects (FINEP) (Agreement No. 01.10.0457.00) and the National Council for Scientific and Technological Development (CNPq) (Case No. 407513/2013-2).
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Conflict of interest
The authors declare that they have no conflict of interest.
- APHA (2011) Standard Methods for the examination of water and waste water. American Public Health AssociationGoogle Scholar
- Bischoff HW, Bold HC (1963) Some soil algae from enchanted rock and related algal species. Phycological Studies, University of Texas IV:1–95Google Scholar
- Ojamae K (2016) Growth physiology and photosynthetic performance of green microalgae mass culture grown in a thin-layer cascade. Masters Thesis, University of TartuGoogle Scholar
- Prokop AK, Bajpai R, Zappi ME (eds) (2017) Algal Biorefineries: Products and Refinery Design, vol 2. Springer, ChamGoogle Scholar
- Richmond A (ed) (2004) Handbook of microalgal culture. Blackwell, LondonGoogle Scholar
- Richmond A, Hu Q (Eds) (2013) Handbook of microalgal culture: applied phycology and biotechnology: second edition. John Wiley & Sons, OxfordGoogle Scholar
- Torzillo G, Giannelli L, Martínez-Roldan AJ, Verdone N, De Filippis P, Scarsella M, Bravi M (2010) Microalgae culturing in thin-layer photobioreactors. Chem Eng Trans 20:265–270Google Scholar