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Suitable unialgal strains of Gracilariopsis chorda and Gracilaria vermiculophylla for hemagglutinin production

  • Hirotaka KakitaEmail author
  • Naoki Yanaoka
  • Hideki Obika
23rd INTERNATIONAL SEAWEED SYMPOSIUM, JEJU

Abstract

The selection of unialgal culture strains suitable as sources of algal hemagglutinins is one of the important factors for success in algal component production. To obtain suitable unialgal culture strains, several Gracilariopsis chorda and Gracilaria vermiculophylla strains were surveyed for hemagglutinating activity, relative growth rates, and fertility difficulty. Fertile tetrasporophyte samples of naturally occurring G. chorda and G. vermiculophylla were collected at each of three different collection sites around Shikoku Island in southwest Japan. Unialgal culture strains were started from isolated tetraspores obtained from each naturally occurring plant. The hemagglutinating activities in algal extracts of the three G. chorda strains (3100–7000 units mg−1) were higher than those of the three G. vermiculophylla strains (920–980 units mg−1). The daily growth rates of the G. chorda strains (8.0–13.8% day−1 at 22 °C) were higher than those of the G. vermiculophylla strains (4.1–4.4% day−1 at 22 °C). Among the unialgal culture strains tested, the one started from isolated tetraspores from G. chorda growing in the Katsuura River had the highest hemagglutinating activity and relative growth rate. This strain also did not become fertile even after a period of 3 years of culture with aeration at 22 °C, in a 14-h light–10-h dark cycle at 60 μmol photons m−2 s−1. Thus, the unialgal culture strain started from isolated tetraspores from G. chorda growing in the Katsuura River seems to be a useful source for hemagglutinin production.

Keywords

Biological production Gracilaria Gracilariopsis Rhodophyta Growth rate Culture 

Notes

Acknowledgments

We are grateful to Emeritus professor Dr. Hirotoshi Yamamoto of Hokkaido University and Professor Dr. Ryuta Terada of Kagoshima University for identifying G. chorda and G. vermiculophylla and for giving advice about unialgal culture procedure.

Funding information

This research was partially supported by the Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for Scientific Research (C), 16K00596, 2016.

References

  1. Beer S, Levy I (1983) Effects of photon fluence rate and light spectrum composition on growth, photosynthesis and pigment relations in Gracilaria sp. J Phycol 19:516–522CrossRefGoogle Scholar
  2. Bird NL, Chen LCM, McLachlan J (1979) Effects of temperature, light and salinity on growth in culture of Chondrus crispus, Furcellaria lumbricalis, Gracilaria tikvahiae (Gigartinales, Rhodophyta) and Fucus serratus (Fucales, Phaeophyta). Bot Mar 22:521–527CrossRefGoogle Scholar
  3. Chirapart A, Ohno M, Sawamura M, Kusunose H (1994) Effect of temperature on growth rate and agar quality of a new member of Japanese Gracilaria in Tosa Bay, sourthern Japan. Jpn J Phycol (Sorui) 42:325–329Google Scholar
  4. Collén J, Guisle-Marsollier I, Léger JJ, Boyen C (2007) Response of the transcriptome of the intertidal red seaweed Chondrus crispus to controlled and natural stresses. New Phytol 176:45–55CrossRefGoogle Scholar
  5. Dickson DM, Jones RGW, Davenport J (1980) Steady state osmotic adaptation in Ulva lactuca. Planta 150:158–165CrossRefGoogle Scholar
  6. Dittami SM, Gravot A, Goulitquer S, Rousvoal S, Peters AF, Bouchereau A, Boyen C, Tonon T (2012) Towards deciphering dynamic changes and evolutionary mechanisms involved in the adaptation to low salinities in Ectocarpus (brown algae). Plant J 71:366–377PubMedGoogle Scholar
  7. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356CrossRefGoogle Scholar
  8. Falkenberg M, Nakano E, Zambotti-Villela L, Zatelli GA, Philippus AC, Imamura KB, Velasquez AMA, Freitas RP, de Freitas TL, Colepicolo P, Graminha MAS (2019) Bioactive compounds against neglected diseases isolated from macroalgae: a review. J Appl Phycol 31:797–823CrossRefGoogle Scholar
  9. Hori K, Miyazawa K, Ito K (1981) Hemagglutinins in marine algae. Bull J Soc Sci Fish 47:793–798CrossRefGoogle Scholar
  10. Ichihara K, Arai S, Shimada S (2009) cDNA cloning of a lectin-like gene preferentially expressed in freshwater from the macroalga Ulva limnetica (Ulvales, Chlorophyta). Phycol Res 57:104–110CrossRefGoogle Scholar
  11. Kain JM (1987) Seasonal growth and photoinhibition in Plocamium cartilagineum (Rhodophyta) off the Isle of Man. Phycologia 26:88–99CrossRefGoogle Scholar
  12. Kakita H, Kitamura T (2003) Hemagglutinating activity in the cultivated red alga Gracilaria chorda. In: Chapman ARO, Anderson RJ, Vreeland VJ, Davision IR (eds) Proceedings of the 17th international seaweed symposium. Oxford University Press, New York, pp 175–182Google Scholar
  13. Kakita H, Fukuoka S, Obika H, Li ZF, Kamishima H (1997) Purification and properties of a high molecular weight hemagglutinin from the red alga, Gracilaria verrucosa. Bot Mar 40:241–247CrossRefGoogle Scholar
  14. Kakita H, Fukuoka H, Obika H, Kamishima H (1999) Isolation and characterization of a fourth hemagglutinin from the red alga, Gracilaria verrucosa. J Appl Phycol 11:49–56CrossRefGoogle Scholar
  15. Kakita H, Kamishima H, Chirapart A, Ohno M (2003) Marine biopolymers from the red algae, Gracilaria spp. In: Fingerrman M, Nagabhushanam R (eds) Recent advances in marine biotechnology. Science Publishers, Enfield, pp 79–109Google Scholar
  16. Kanoh H, Kitamura T, Kobayashi Y (1992) A sulfated proteoglycan from the red alga Gracilaria verrucosa is a hemagglutinin. Comp Biochem Physiol 102B:445–449Google Scholar
  17. Kocourek J (1986) Historical background. In: Sharon N, Goldstain IJ (eds) IE Liener. The lectins. Academic Press, New York, pp 1–32Google Scholar
  18. Laing WA, Christeller JT, Terzaghi BE (1989) The effect of temperature, photon flux density and nitrogen on growth of Gracilaria sordida Nelson (Rhodophyta). Bot Mar 32:439–445CrossRefGoogle Scholar
  19. Lapointe BE (1981) The effects of light and nitrogen on growth, pigment content, and biochemical composition of Gracilaria foliifera v. angustissima (Gigartinales, Rhodophyta). J Phycol 17:90–95CrossRefGoogle Scholar
  20. Lowry OH, Rosebrough NJ, Farr AL, Randall RL (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275PubMedPubMedCentralGoogle Scholar
  21. McLachlan J, Bird CJ (1984) Geographical and experimental assessment of the distribution of Gracilaria species (Rhodophyta, Gigartinales) in relation to temperature. Helgol Meeresun 38:319–334CrossRefGoogle Scholar
  22. Mostaert AS, Karsten UF, King RJ (1995) Physiological responses of Caloglossa leprieurii (Ceramiales, Rhodophyta) to salinity stress. Phycol Res 43:215–222CrossRefGoogle Scholar
  23. Nelson WA (1989) Phenology of Gracilaria sordida W. Nelson populations. Reproductive status, plant and population size. Bot Mar 32:41–51CrossRefGoogle Scholar
  24. Ogata E, Matsui T, Nakamura H (1972) The life cycle of Gracilaria verrucosa (Rhodophyceae, Gigartinales) in vitro. Phycologia 11:75–80CrossRefGoogle Scholar
  25. Provasoli L (1968) Media and prospects for the cultivation of marine algae. In: Watanabe A, Hattori A (eds) Cultures and collections of algae. Jap. Soc. Plant Physiol, Tokyo, pp 63–75Google Scholar
  26. Shiomi K, Yamanala H, Kikuchi T (1981) Purification and physicochemical properties of a hemagglutinin (GVA-1) in the red alga Gracilaria verrucosa. Bull J Soc Sci Fish 47:1079–1084CrossRefGoogle Scholar
  27. Smit AJ (2004) Medicinal and pharmaceutical uses of seaweed natural products: a review. J Appl Phycol 16:245–262CrossRefGoogle Scholar
  28. Takahashi Y, Katagiri S (1987) Seasonal variation of the hemagglutinating activities in the red alga Gracilaria verrucosa. Nippon Suisan Gakkaishi 53:2133–2137CrossRefGoogle Scholar
  29. Teo SS, Ho CL, Teoh S, Rahim RA, Phang SM (2009) Transcriptomic analysis of Gracilaria Changii (Rhodophyta) in response to hyper- and hypoosmotic stresses. J Phycol 45:1093–1099CrossRefGoogle Scholar
  30. Weig A, Deswarte C, Chrispeels M (1997) The major intrinsic protein family of Arabidopsis has 23 members that from three distinct groups with functional aquaporins in each group. Plant Physiol 114:1347–1357CrossRefGoogle Scholar
  31. Winer BJ, Brown DR, Michels KM (1991) Statistical principles in experimental design, 3rd edn. McGraw-Hill, New York, pp 153–198Google Scholar
  32. Yamamoto H, Sasaki J (1987) Crossing experiments between populations and so-called Gracilaria verrucosa (Huds.) Papenfuss from two localities, Shinori and Kikonai in Hokkaido. Bull Fac Hokkaido Univ 38:335–338Google Scholar
  33. Yokoya NS, Oliveira EC (1992) Temperature responses of economically important red algae and their potential for mariculture in Brazilian waters. J Appl Phycil 4:339–345CrossRefGoogle Scholar
  34. Yokoya NS, Kakita H, Obika H, Kitamura T (1999) Effects of environmental factors and plant growth regulators on growth of the red alga Gracilaria vermiculophylla from Shikoku Island, Japan. Hydrobiologia 398:339–347CrossRefGoogle Scholar
  35. Yoshida T, Yoshinaga K (2010) Check list of marine algae of Japan (revised in 2010). Jpn J Phycol (Sorui) 58:69–122Google Scholar
  36. Yoshida T, Yoshinaga K, Nakajima Y (2000) Check list of marine algae of Japan (revised in 2000). Jpn J Phycol (Sorui) 48:113–166Google Scholar

Copyright information

© Springer Nature B.V. 2020

Authors and Affiliations

  1. 1.Graduate School of Integrated Basic SciencesNihon UniversityTokyoJapan
  2. 2.Health Research Institute, National Institute of Advanced Industrial Science and TechnologyTakamatsuJapan

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