Individual and combined supplementation of carbon sources for growth augmentation and enrichment of lipids in the green microalga Tetradesmus obliquus
Microalgae with high lipid productivity offer great promise as a potential source of biodiesel, an alternative to fossil diesel. Since a target of both high biomass yield and lipid content is difficult to attain with the classical photoautotrophic mode of nutrition, mixotrophy has evolved as a preferred mode for enhancement of growth in microalgae. In the present study, this strategy was explored extensively with six different exogenous carbon sources in eight different concentrations to investigate the effect of these carbon sources on the biomass and lipid production potential of the green alga Tetradesmus obliquus (SAG 276-3a). To further augment the effect of mixotrophy on the growth and lipid accumulation of the test microalga, selected carbon sources with their specific concentrations showing enhancement in both biomass yield and lipid accumulation in the first phase of the experiment were interacted with each other. Significant rise in biomass yield by twofold was seen in the case of 0.16% citrate + 0.08% bicarbonate interaction, whereas for lipid accumulation, maximum rise in lipid content (% dry cell wt.) by 2.3-fold and lipid yield (g L-1) by 4.5-fold was observed in the case of 0.16% acetate + 0.16% citrate-supplemented cultures. The biodiesel samples as analyzed using GC–MS were predominated with saturated and monounsaturated fatty acid methyl esters. Fuel property analysis of the biodiesel samples found them to be well suited to the Indian, American (ASTM), and European (EN) biodiesel standards.
KeywordsBiodiesel Fuel properties Mixotrophy Tetradesmus obliquus Chlorophyceae
Financial support from Indian Institute of Technology Kharagpur, West Bengal, and NASF, Indian Council of Agricultural Research, New Delhi, India, are thankfully acknowledged.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- American Oil Chemists’ Society (AOCS) (1998) Iodine value of fats and oils. Wijs method. In: Firestone D (ed) Official methods and recommended practices of the American Oil Chemists’ Society, 5th edn. AOCS Press, Champaign, USA, pp 1–25Google Scholar
- American Standards for Testing of Materials ASTM (2003) D 4052-96. D 240-02:482–474Google Scholar
- Bajwaa K, Silambarasan T, Smita BNR (2016) Effect of glucose supplementation and mixotrophic effects of glycerol and glucose on the production of biomass, lipid yield and different physiological, biochemical attributes of Chlorella pyrenoidosa. J Algal Biomass Util 7:93–103Google Scholar
- Cecchin M, Benfatto S, Griggio F, Mori A, Cazzaniga S, Vitulo N, Delledonne M, Ballottari M (2018) Molecular basis of autotrophic versus mixotrophic growth in Chlorella sorokiniana. Sci Rep 8:1–13Google Scholar
- Cohen Z, Khozin-Goldberg I (2005) Searching for PUFA-rich microalgae, In Single Cell Oils. In: Cohen Z, Ratledge C, Champaign IL (eds) American Oil Chemists’ Society, pp 53–72Google Scholar
- Degrenne B, Pruvost S, Christophe G, Cornet J F, Cogne G, Legrand J (2010) Investigation of the combined effects of acetate and photobioreactor illuminated fraction in the induction of anoxia for hydrogen production by Chlamydomonas reinhardtii. Int J Hydrogen Energy 35:10741–10749CrossRefGoogle Scholar
- Deng X, Cai J, Fei X (2013) Effect of the expression and knockdown of citrate synthase gene on carbon flux during triacylglycerol biosynthesis by green algae Chlamydomonas reinhardtii. BMC Biochem 38:1–11Google Scholar
- Estévez-Landazábal LL, Barajas-Solano AF, Barajas-Ferreira C, Kafarov V (2013) Improvement of lipid productivity on Chlorella vulgaris using waste glycerol and sodium acetate. Ciencia, Tecnología Futuro 5:113–126Google Scholar
- European Standard EN 14214 (2003) European standards for biodiesel. http://www.din.de. Accessed on 8.07.2016.
- Indian Standard IS: 15607 (2005) Biodiesel specification. Bureau of Indian Standards. http://www.bis.org.in. Accessed on 5.05.2016.
- International Energy Agency IEA (2015) Energy and climate change. World Energy Outlook Special Report. OECD/IEA, Paris, pp 17–31Google Scholar
- Kong WB, Yang H, Cao YT, Song H, Hua SF, Xia CG (2013) Effect of glycerol and glucose on the enhancement of biomass, lipid and soluble carbohydrate production by Chlorella vulgaris in mixotrophic culture. Food Technol Biotechnol 51:62–69Google Scholar
- Ministry of Environment, Forest and Climate change (MoEFCC) (2014) Statement by the Hon’ble Minister at the high level segment of UNFCC COP-20. http://www.moef.nic.in/content/statement-hon%E2%80%99ble-minister-high-level-segment-unfccc-cop-20-december-9-2014.
- Mokashi K, Shetty V, George SA, Sibi G (2016) Sodium bicarbonate as inorganic carbon source for higher biomass and lipid production integrated carbon capture in Chlorella vulgaris. Arch Life Sci 10:111–117Google Scholar
- Nelson DL, Cox M (2000) Amino acid oxidation and the production of urea. In: Ryan M, Strange L, Neal V(eds.) Lehninger principles of biochemistry. Worth Publishers, New York, p 643Google Scholar
- Nelson DR, Rinne RW (1977) The role of citrate in lipid synthesis in developing soybean cotyledons. Plant Cell Physiol 18:1021–1027Google Scholar
- Rai MP, Gupta S (2016) Growth and lipid production from Scenedesmus sp. under mixotrophic condition for bioenergy application. In: Kumar S, Khanal SK, Yadav YK (eds) Proceedings of the first international conference on recent advances in bioenergy research. Springer Nature, India, pp 159–168CrossRefGoogle Scholar
- Rismani-Yazdi H, Haznedaroglu BZ, Bibby K, Peccia J (2011) Transcriptome sequencing and annotation of the microalgae Dunaliella tertiolecta: pathway description and gene discovery for production of nextgeneration biofuels. BMC Genomics 12:1–18Google Scholar
- Subramanian S, Barry AN, Pieris S, Sayre RT (2013) Comparative energetics and kinetics of autotrophic lipid and starch metabolism in chlorophytic microalgae: implications for biomass and biofuel production. Biotechnol Biofuels 150:1–12Google Scholar
- Venkatesan H, Sivamani S, Sampath S, Gopi V, Dinesh Kumar M (2017) A comprehensive review on the effect of nano metallic additives on fuel properties, engine performance and emission characteristics. Int J Renew Energy Res 7:825–843Google Scholar
- Villanova V, Fortunato AE, Singh D, Bo DD, Conte M, Obata T, Jouhet J, Fernie AR, Marechal E, Falciatore A, Pagliardini J, Monnier AL, Poolman M, Curien G, Petroutsos D, Finazzi G (2017) Investigating mixotrophic metabolism in the model diatom Phaeodactylum tricornutum. Philos Trans R Soc B 372:1–14CrossRefGoogle Scholar