Advertisement

Scaling the production of Monostroma sp. by optimizing culture conditions

  • Monica Gajanan KavaleEmail author
  • Bhumi Italiya
  • V. Veeragurunathan
Article

Abstract

Laboratory culture experiments were performed to optimize conditions for scaled-up production of Monostroma sp. The effects of growth determining factors like salinity (15–45 psu), photoperiod (8:16–16:8 L/D), light intensity (2–60 μmol photons m−2 s−1), and temperature (15–35 °C) on the growth of Monostroma sp. were assessed. Culture results indicated high tolerance capacity of Monostroma sp. to all levels in salinity, photoperiod, light intensity and temperature tested. ANOVA results confirmed significant impact of salinity, photoperiod, light intensity and temperature on growth rate (p < 0.05). The maximum growth rate was observed in the range of 5.73 to 14.41% day−1 which obtained in 35 psu salinity, 25 °C temperature, 14:10 (L/D) photoperiod, 60 μmol photons m−2 s−1 light intensity level and 1/4 MP1 medium. The modified 1/4MP1 medium was found to be suitable for enhanced growth of Monostroma sp. Outdoor tank culture with 1/4 MP1 medium showed maximum daily growth rate (14.38 ± 0.32% day−1).

Keywords

Monostroma Chlorophyta Scale-up Growth rate Salinity Light intensity Photoperiod Temperature 

Notes

Acknowledgments

The authors expressed their sincere thanks to Dr. Amitava Das, Director, CSMCRI for his encouragement to pursue this work. The authors expressed their sincere thanks to Dr. C.R.K. Reddy, former Divisional Chair, Discipline of Marine Biotechnology and Ecology, CSMCRI for his keen interest in the work. We also thank Dr. P. K. Agarwal, Divisional Chair, Biotechnology and Phycology Division, CSMCRI for providing facilities. The authors are grateful to Dr. V. A. Mantri Group Head, Division of Biotechnology and Phycology for his valuable suggestions while writing this manuscript. This contribution has CSIR-CSMCRI PRIS registration number (CSIR-CSMCRI-211/2019).

Funding information

The financial support by the Council of Scientific and Industrial Research (CSIR), New Delhi under in-house project (MLP022) is gratefully acknowledged.

Supplementary material

10811_2019_1922_MOESM1_ESM.docx (19 kb)
ESM 1 (DOCX 19 kb)

References

  1. Abèlard C (1979) Influence de divers milieux sur la croissance du thalle d’Apoglossum ruscifolium (Turner) J. Agardh, (Rhodophycées, Céramiales). Rev Algol N S 15:343–357Google Scholar
  2. Bolton JJ, Robertson-Andersson DV, Shuuluka D, Kandjengo L (2009) Growing Ulva (Chlorophyta) in integrated system as a commercial crop for abalone feed in South Africa: a SWOT analysis. J Appl Phycol 21:575–583CrossRefGoogle Scholar
  3. Braga MRA (1997) Recruitment of two species of monostromatic blade-like chlorophytes, Monostroma sp. and Ulvaria oxysperma (Ulvales), in Sao Paulo State, Brazil. Phycol Res 45:153–161CrossRefGoogle Scholar
  4. Braga MR, Fujii MT, Cordeiro-Marino M (1997) Monostromatic green algae (Ulvales, Chlorophyta) of Sao Paulo and Parana states: distribution, growth and reproduction. Rev Bras Bot 20:197–203CrossRefGoogle Scholar
  5. Choi TS, Kang EJ, Kim JH, Kim KY (2010) Effect of salinity on growth and nutrient uptake of Ulva pertusa (Chlorophyta) from an eelgrass bed. Algae 25:17–26CrossRefGoogle Scholar
  6. Cordeiro-Marino M, Braga MRA, Fujii MT, Guimaraes SMBP, Mitsugui EM (1993) Monostromatic green algae from Espìrito Santo state: life history, growth and reproduction in culture. Rev Bras Biol 53:285–293Google Scholar
  7. Dawes CP (1995) Suspended cultivation of Gracilaria in the sea. J Appl Phycol 8:310–313Google Scholar
  8. Deshmukhe G, Dhargalkar VK, Untawale AG (1998) Life history and culture studies of Monostroma oxyspermum (Kutz.) Doty (Monostromataceae, Chlorophyceae) growing in estuarine conditions along the central west coast of India. Curr Sci 75:1302–1303Google Scholar
  9. Ding L, Ma Y, Huang B, Chen S (2013) Effects of seawater salinity and temperature on growth and pigment contents in Hypnea cervicornis J. Agardh (Gigartinales, Rhodophyta). Bio Med Res IntGoogle Scholar
  10. FAO (Food and Agriculture Organization of the United Nations) (2018) The global status of seaweed production, trade and utilization, vol 124. FAO, RomeGoogle Scholar
  11. Guo Z, Mathieson AC (1992) Physiological ecology of four ulvoid green algae. Bot Mar 35:523–533CrossRefGoogle Scholar
  12. Kirst GO (1989) Salinity tolerance of eukaryotic marine algae. Annu Rev Plant Physiol 40:21–53Google Scholar
  13. Li H, Mao W, Zhang X, Qi X, Chen Y, Chen Y, Xu J, Zhao C, Hou Y, Yang Y, Li N, Wang C (2011) Structural characterization of an anticoagulant-active sulfated polysaccharide isolated from green alga Monostroma latissimum. Carbohydr Polym 85:394–400CrossRefGoogle Scholar
  14. Lobban CS, Harrison PJ, Duncan JJ (1985) The physiological ecology of seaweeds. Cambridge University Press, Cambridge, p 242Google Scholar
  15. Lüning K (1981) Light. In: Lobban CS, Wynne MJ (eds) The biology of seaweeds. Blackwell, Oxford, pp 326–355Google Scholar
  16. Mata L, Magnusson M, Paul NA, de Nys R (2016) The intensive land-based production of the green seaweeds Derbesia tenuissima and Ulva ohnoi: biomass and bioproducts. J Appl Phycol 28:365–375CrossRefGoogle Scholar
  17. Mathieson AC, Penniman CA (1986) Species composition and seasonality of New England seaweeds along an open coastal-estuarine gradient. Bot Mar 29:161–176Google Scholar
  18. Mathieson AC, Penniman CA (1991) Floristic patterns and numerical classification of New England estuarine and open coastal seaweed populations. Nova Hedwigia 52:453–485Google Scholar
  19. McDermid KJ, Stuercke B (2003) Nutritional composition of edible Hawaiian seaweeds. J Appl Phycol 15:513–524CrossRefGoogle Scholar
  20. Mclaclan J (1982) Inorganic nutrient of marine macroalgae in culture. In Srivastava LM (ed) Synethetic and Degradative Process in Marine Macrophytes, Berlin, Walter de Gruyter, pp 71-98Google Scholar
  21. Mosquera-Murillo Z, Peña-Salamanca EJ (2016) Effect of salinity on growth of the green alga Caulerpa sertularioides (Bryopsidales, Chlorophyta) under laboratory conditions. Hidrobiológica 26:277–282CrossRefGoogle Scholar
  22. Neori A, Chopin T, Troell M, Buschmann AH, Kraemer GP, Halling C, Shpigel M, Yarish C (2004) Integrated aquaculture: rationale, evolution and state of the art emphasizing seaweed biofiltration in modern mariculture. Aquaculture 231:361–391CrossRefGoogle Scholar
  23. Neori A, Shpigel M, Guttman L, Israel A (2017) Development of polyculture and integrated multitrophic aquaculture (IMTA) in Israel: a review. Israel J Aquacult 69:1385–1404Google Scholar
  24. Ohno M (1993) Cultivation of the green alga, Monostroma and Enteromorpha ‘Aonori’. In: Ohno M, Critchley AT (eds) Seaweed cultivation and marine ranching. Japan International Cooperation Agency, Japan, pp 7–16Google Scholar
  25. Ohno M (1995) Cultivation of Monostroma nitidum (Chlorophyta) in a river estuary, Southern Japan. J Appl Phycol 7:207–213CrossRefGoogle Scholar
  26. Ohno M, Nozawa K (1972) Observation of spore formation and photosynthetic activities on Monostroma nitidum. Bull Jpn Soc Phyc 20:30–35Google Scholar
  27. Ohno M, Triet VD (1997) Artificial seeding of the green seaweed Monostroma for cultivation. J Appl Phycol 9:417–423CrossRefGoogle Scholar
  28. Oza RM, Joshi HV, Parekh RG, Chauhan VD (1983) Preliminary observation on a Monostroma sp. from Okha coast, Gujarat. Ind J Mar Sci 12:115–117Google Scholar
  29. Pellizzari F, Reis RP (2011) Seaweed cultivation on the southern and southeastern Brazilian coast. Braz J Pharmacog 21:305–312CrossRefGoogle Scholar
  30. Pellizzari FM, Absher T, Yokoya NS, Oliveira EC (2007) Cultivation of the edible green seaweed Gayralia (Chlorophyta) in Southern Brazil. J Appl Phycol 19:63–69CrossRefGoogle Scholar
  31. Pellizzari F, Oliveira EC, Yokoya NS (2008) Life-history, thallus ontogeny, and the effect of temperature, irradiance and salinity on growth of the edible green seaweed Gayralia spp. (Chlorophyta) from southern Brazil. J Appl Phycol 20:75–82CrossRefGoogle Scholar
  32. Provasoli L, McLaughlin J, Droop M (1957) The development of artificial media for marine algae. Arch Mikrobiol 25:392–248CrossRefGoogle Scholar
  33. Reddy CRK, Dipakkore S, Rajakrishna Kumar G, Jha B, Cheney DP Fujita Y (2006) An improved enzyme preparation for rapid mass production of protoplasts as a seed stock for aquaculture of macrophytic marine green algae. Aquaculture 260:290–297CrossRefGoogle Scholar
  34. Risso SC, Escudero S, Belchior M, Portella d, Fajardo M (2003) Chemical composition and seasonal fluctuations of the edible green seaweed, Monostroma undulatum Wittrock, from the southern Argentina coast. Arch Latinoam Nutr 53:306–311PubMedGoogle Scholar
  35. Schreiber E (1942) Über Monostroma bullosum Thur. und Monostroma grevillei (Thur.) und Cladophora rupestris (L). Planta 32:414–417CrossRefGoogle Scholar
  36. Tasende MG, Fraga MI (1999) Growth of Chondrus crispus Stackhouse (Rhodophyta, Gigartinaceae) in laboratory culture. Ophelia 51:202–213CrossRefGoogle Scholar
  37. Tatewaki M (1969) Culture studies on the life history of some species of the genus Monostroma. Scientific Papers of the Institute of Algological Research, Hokkaido University, Japan 6:1–56Google Scholar
  38. Toma T (1991) Monostroma spp. (‘hitoe-gusa’). Shokita (eds) aquaculture in tropical areas. Midor Ishodo, pp 36–44Google Scholar
  39. Untawale AG, Agadi VV, Dhargalkar VK (1980) Occurrence of genus Monostroma (Ulvales, Chlorophyta) from Ratnagiri (Maharashtra). Mahasagar-Bull Nat Inst Oceanogr 13:179–181Google Scholar
  40. Wilkinson M (1980) Estuarine benthic algae and their environments: a review. In: Irvine JH, Irvine DEG, Farnham EF (eds) The shore environment, vol 2. Ecosystems. Academic Press, New York, pp 425–486Google Scholar
  41. Wilson KL, Kay LM, Schmidt AL, Lotz HK (2015) Effects of increasing water temperatures on survival and growth of ecologically and economically important seaweeds in Atlantic Canada: implications for climate change. Mar Biol 162:2431–2444CrossRefGoogle Scholar
  42. Yu CH, Lim PE, Phang SW (2013) Effects of irradiance and salinity on the growth of carpospore-derived tetrasporophytes of Gracilaria edulis and Gracilaria tenuistipitata var liui (Rhodophyta). J Appl Phycol 25:787–794CrossRefGoogle Scholar
  43. Zhang HJ, Mao WJ, Fang F, Li HY, Sun HH, Chen Y, Qi XH (2008) Chemical characteristics and anti-coagulant activities of a sulfated polysaccharide and its fragments from Monostroma latissimum. Carbohydr Polym 71:428–434CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Monica Gajanan Kavale
    • 1
    Email author
  • Bhumi Italiya
    • 2
  • V. Veeragurunathan
    • 1
    • 3
  1. 1.Division of Biotechnology and PhycologyCSIR-Central Salt & Marine Chemicals Research InstituteBhavnagarIndia
  2. 2.Bhagwan Mahavir College of Science and TechnologySuratIndia
  3. 3.Academy of Scientific and Innovative Research (AcSIR)CSIR-Central Salt & Marine Chemicals Research InstituteBhavnagarIndia

Personalised recommendations