Scaling the production of Monostroma sp. by optimizing culture conditions

  • Monica Gajanan KavaleEmail author
  • Bhumi Italiya
  • V. Veeragurunathan


Laboratory culture experiments were performed to optimize conditions for scaled-up production of Monostroma sp. The effects of growth determining factors like salinity (15–45 psu), photoperiod (8:16–16:8 L/D), light intensity (2–60 μmol photons m−2 s−1), and temperature (15–35 °C) on the growth of Monostroma sp. were assessed. Culture results indicated high tolerance capacity of Monostroma sp. to all levels in salinity, photoperiod, light intensity and temperature tested. ANOVA results confirmed significant impact of salinity, photoperiod, light intensity and temperature on growth rate (p < 0.05). The maximum growth rate was observed in the range of 5.73 to 14.41% day−1 which obtained in 35 psu salinity, 25 °C temperature, 14:10 (L/D) photoperiod, 60 μmol photons m−2 s−1 light intensity level and 1/4 MP1 medium. The modified 1/4MP1 medium was found to be suitable for enhanced growth of Monostroma sp. Outdoor tank culture with 1/4 MP1 medium showed maximum daily growth rate (14.38 ± 0.32% day−1).


Monostroma Chlorophyta Scale-up Growth rate Salinity Light intensity Photoperiod Temperature 



The authors expressed their sincere thanks to Dr. Amitava Das, Director, CSMCRI for his encouragement to pursue this work. The authors expressed their sincere thanks to Dr. C.R.K. Reddy, former Divisional Chair, Discipline of Marine Biotechnology and Ecology, CSMCRI for his keen interest in the work. We also thank Dr. P. K. Agarwal, Divisional Chair, Biotechnology and Phycology Division, CSMCRI for providing facilities. The authors are grateful to Dr. V. A. Mantri Group Head, Division of Biotechnology and Phycology for his valuable suggestions while writing this manuscript. This contribution has CSIR-CSMCRI PRIS registration number (CSIR-CSMCRI-211/2019).

Funding information

The financial support by the Council of Scientific and Industrial Research (CSIR), New Delhi under in-house project (MLP022) is gratefully acknowledged.

Supplementary material

10811_2019_1922_MOESM1_ESM.docx (19 kb)
ESM 1 (DOCX 19 kb)


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Monica Gajanan Kavale
    • 1
    Email author
  • Bhumi Italiya
    • 2
  • V. Veeragurunathan
    • 1
    • 3
  1. 1.Division of Biotechnology and PhycologyCSIR-Central Salt & Marine Chemicals Research InstituteBhavnagarIndia
  2. 2.Bhagwan Mahavir College of Science and TechnologySuratIndia
  3. 3.Academy of Scientific and Innovative Research (AcSIR)CSIR-Central Salt & Marine Chemicals Research InstituteBhavnagarIndia

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