Journal of Applied Phycology

, Volume 31, Issue 5, pp 2933–2940 | Cite as

Diets containing residual microalgae biomass protect fishes against oxidative stress and DNA damage

  • Antonio Ernesto Meister Luz Marques
  • Rafael Ernesto Balen
  • Letícia da Silva Pereira Fernandes
  • Cintya Marques Motta
  • Helena Cristina Silva de Assis
  • Dhyogo Miléo Taher
  • Fábio Meurer
  • José Viriato Coelho Vargas
  • André Bellin MarianoEmail author
  • Marta Margarete Cestari


Microalgae are major antioxidant producers and feed containing these substances is known to be beneficial. Microalgae cultivation is an alternative way to produce biodiesel and, after oil extraction, residual algal biomass (RAB) is obtained. The RAB was tested as an ingredient in fish feed production and its safety evaluation is important to prevent risks to fish health. This study aim was to evaluate, through biochemical and genetic biomarkers, the safety of RAB in catfish, Rhamdia quelen, feed. Acutodesmus obliquus microalgae RAB, cultivated in Chu medium, was used in feed formulation. A standard feed without RAB (0%) was produced, and three other feeds were enriched with RAB in 1, 2, and 3% proportion. Each feed kind was given to a 15 R. quelen fingerling group for 60 days. The evaluated biochemical biomarkers were superoxide dismutase (SOD) and catalase (CAT) activities, lipid peroxidation (LPO) in the liver, and acetilcolinesterase (AChE) activity in the brain and muscles. The genetic biomarkers analyzed were halo assay in erythrocytes, comet assay in erythrocytes, liver and brain, and piscine micronucleus. The SOD activity was increased in the 3% group; CAT activity and LPO levels were not different among the groups. In the comet assay, a significant decrease in DNA damage in erythrocytes (2 and 3%) and liver tissue (3%) was observed. In the brain, DNA damage was not observed. These results corroborate that as the RAB amount increased, the organisms showed a potential antioxidant effect, as the 3% RAB feed had the best results.


Antioxidant feed Carotenoids Chlorophyll Acutodesmus Residual algal biomass Supplementation 



The authors would like to thank MSc. Camila da Costa Senkiv and Lúcia Gil for providing language and writing support, and the assistance of the following laboratories from Federal University of Paraná (UFPR): Animal Cytogenetics and Environmental Mutagenesis Lab., Aquaculture Technology Lab., Environmental Toxicology Lab. and NPDEAS.

Funding information

This work was financially supported by CNPq and CAPES under the grant [grant numbers 40001016006P1].

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Antonio Ernesto Meister Luz Marques
    • 1
    • 2
  • Rafael Ernesto Balen
    • 3
  • Letícia da Silva Pereira Fernandes
    • 4
  • Cintya Marques Motta
    • 2
  • Helena Cristina Silva de Assis
    • 4
  • Dhyogo Miléo Taher
    • 1
  • Fábio Meurer
    • 3
  • José Viriato Coelho Vargas
    • 1
    • 5
  • André Bellin Mariano
    • 1
    • 6
    Email author
  • Marta Margarete Cestari
    • 1
    • 2
  1. 1.Centre for Research and Development of Sustainable Energy (NPDEAS)Federal University of Paraná (UFPRCuritibaBrazil
  2. 2.Department of Genetics/UFPR, Animal Cytogenetics and Environmental Mutagenesis LaboratoryCuritibaBrazil
  3. 3.Department of Zoology/UFPR, Aquaculture Technology LaboratoryCuritibaBrazil
  4. 4.Department of Pharmacology/UFPR, Environmental Toxicology LaboratoryCuritibaBrazil
  5. 5.Department of Mechanical Engineering/UFPRCuritibaBrazil
  6. 6.Departamento de Engenharia Elétrica, Centro Politécnico, Setor de Tecnologia, Avenida Coronel Francisco H. dos Santos S/N, CP 19011, CEP 81531-980CuritibaBrazil

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