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Journal of Applied Phycology

, Volume 31, Issue 5, pp 2833–2844 | Cite as

Assimilation of inorganic nitrogen for scaling up Desmodesmus communis (Scenedesmaceae) biomass production

  • Laura PezzolesiEmail author
  • Matilde Mazzotti
  • Silvana Vanucci
  • Rossella Pistocchi
Article

Abstract

The feasibility of the green alga Desmodesmus communis for biomass production was investigated, firstly testing different nitrogen forms in the growth medium and the effect of CO2-enriched air supply, secondarily scaling up the cultivation system in 70 L photobioreactors (PBRs). Maximum nitrogen uptake rate obtained in the performed kinetic experiment was higher for ammonium than for nitrate (188.0 vs 11.7 μmol g−1 h−1); however, D. communis cultured in PBRs with only aeration grew faster with nitrate reaching a biomass yield (1.23 g L−1) and a productivity (0.036 g L−1 day−1) about twofold higher than with ammonium, which caused a pH decrease in the medium affecting the algal growth. CO2 supply allowed algal growth optimization, maintaining a high productivity with both nitrogen sources, slightly higher with nitrate (0.050 vs 0.038 g L−1 day−1). Additionally, nitrate-supplied cells showed higher lipids (19.0 vs 9.4%) and proteins (33.0 vs 27.2%) values than those grown with ammonium. The semi-continuous scaled-up cultivation performed for 5 months attests the potential utilization of this species for valuable algal biomass production exploitable in various industrial applications.

Keywords

Desmodesmus Chlorophyta Nitrogen Algal biomass Photobioreactor 

Notes

Acknowledgements

This study was supported by the framework of the APQ Ricerca Intervento a “Sostegno dello sviluppo dei Laboratori di ricerca nei campi della nautica e dell’energia per il Tecnopolo di Ravenna” “Energia, parte Biomasse” between Università di Bologna and Regione Emilia Romagna (Italy). The authors are very grateful to the reviewers for the careful reading of the paper, and their valuable comments and corrections that help to improve the manuscript.

Authors’ contributions

LP and MM did the conception and design of the study and the analysis and interpretation of the data. LP and, partially, MM wrote the article. SV and RP helped in the interpretation of the data. SV did the critical revision of the article for important intellectual content. RS did the final approval of the article and obtained the funding to perform the work. All authors approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Biological, Geological and Environmental Sciences (BiGeA)University of BolognaRavennaItaly
  2. 2.Department of Chemical, Biological, Pharmaceutical and Environmental Sciences (ChiBioFarAm)University of MessinaMessinaItaly

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