Journal of Applied Phycology

, Volume 31, Issue 5, pp 3245–3254 | Cite as

Substitution effect of the combined fouling macroalgae Ulva australis and Sargassum horneri for Undaria pinnatifida in formulated diets on growth and body composition of juvenile abalone (Haliotis discus, Reeve 1846) subjected to air exposure stressor

  • Most. Waheda Rahman Ansary
  • Seong Il Baek
  • Hae Seung Jeong
  • Ki Wook Lee
  • Sung Hwoan ChoEmail author
  • Hee Sung Kim
  • Min-Seok Jwa


The effect of substituting the combined macroalgae Ulva australis and Sargassum horneri for Undaria pinnatifida in formulated diets on growth and body composition of abalone subjected to air exposure stressor was investigated. A total of 1260 juvenile abalone were distributed into 21 cages. Six formulated diets were prepared. The control (CUS0) diet contained 20% U. pinnatifida. Twenty, 40, 60, 80, and 100% of U. pinnatifida were substituted with an equal amount of the combined U. australis and S. horneri, referred to as the CUS20, CUS40, CUS60, CUS80, and CUS100 diets, respectively. Finally, dry U. pinnatifida was prepared to compare the growth performance of abalone. Abalone were fed with one of the experimental diets once a day for 16 weeks and then subjected to air stressor for 24 h. The cumulative mortality of abalone was monitored for the following 4 days after 24 h of air exposure. Abalone fed all formulated diets attained higher survival, weight gain, and specific growth rate (SGR) than U. pinnatifida. Abalone fed the CUS100 diet achieved greatest weight gain and SGR, followed by the CUS80 and CUS60 diets. The greatest shell growth and heaviest soft-body weight were obtained in abalone fed the CUS100 diet. Proximate composition of the soft body of abalone, except for moisture content, was not affected by the experimental diets. The cumulative mortality of abalone fed the U. pinnatifida was higher than that of abalone fed all formulated diets at 84 h until the end of the 4-day post observation. The lowest cumulative mortality was obtained in abalone fed the CUS80 diet at the end of the 4-day post observation. Therefore, U. pinnatifida could be completely replaced with the combined U. australis and S. horneri in abalone (H. discus) feed.


Abalone (Haliotis discusUndaria pinnatifida Substitution effect Sargassum horneri Ulva australis Formulated diet Air exposure stressor 



This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (2017R1A2B4009773). This work was also supported by a grant from the National Institute of Fisheries Science, Republic of Korea (R2019010).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Convergence Study on the Ocean Science and TechnologyKorea Maritime and Ocean UniversityBusanSouth Korea
  2. 2.Division of Marine BioscienceKorea Maritime and Ocean UniversityBusanSouth Korea
  3. 3.East Sea Fisheries Research InstituteNational Institute of Fisheries ScienceBusanSouth Korea
  4. 4.Jeju Research InstituteJejuSouth Korea

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