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Phlorotannins from Padina tetrastromatica: structural characterisation and functional studies

  • Divya Nair
  • Muralidharan Vanuopadath
  • Akshaya Balasubramanian
  • Akshay Iyer
  • Shankar Ganesh
  • Abhishek Nair Anil
  • Vijesh Vikraman
  • Prasanth Pillai
  • Chinchu Bose
  • Bipin Gopalakrishnan Nair
  • Jayashree Gopalakrishna Pai
  • Sudarslal Sadasivan NairEmail author
Article

Abstract

In this study, LC–MS/MS-based structural characterisation of phlorotannins from Padina tetrastromatica, a marine brown macroalga collected from South-West coastal region of Kerala, and its bioactivities are presented. The tandem mass spectrometric data revealed a series of phlorotannins with degree of polymerisation ranging from 2 to 18. The characteristic neutral loss of tandem mass spectra further confirmed that these molecules belong to fucophlorethol class of phlorotannins. DPPH assay of the HPLC-purified, phlorotannin-enriched fraction possesses significant free radical-scavenging activity. Cell viability assay indicated that phlorotannin concentration ranging from 1.5 to 50.0 μg mL−1 is non-toxic to THP-1 cell lines. Anti-inflammatory assay performed through gelatin zymography confirmed that phlorotannins ameliorated high-glucose-induced pro-MMP-9 expression in a dose-dependent manner whereas the level of pro-MMP-2 remains unaltered. The antimicrobial assays carried out using both the crude and HPLC-purified phlorotannin fraction showed its anti-MRSA potential.

Keywords

Padina tetrastromatica Phaeophyceae Phlorotannin Mass spectrometry DPPH assay Anti-inflammatory 

Notes

Acknowledgements

The authors would like to acknowledge Dr. Nataraja Panicker for helping with the identification of the algal sample; Dr. Anil Kumar V, Professor, Microbiology, School of Medicine, Amrita Institute of Medical Sciences, for providing the methicillin-resistant Staphylococcus aureus strain; Dr. Ashoke Banerji and Dr. Pandu Rangan, School of Biotechnology, for the solvent extraction process; and Amrita Agilent Analytical Research Centre and Mr. Nithin Sajeev for helping with the mass spectrometry data collection.

Funding information

The financial support provided to MV and DN in the form of fellowship by Amrita Vishwa Vidyapeetham is duly acknowledged. All the authors are grateful to Amrita Vishwa Vidyapeetham for providing financial assistance in executing this project.

Supplementary material

10811_2019_1792_MOESM1_ESM.docx (1.9 mb)
ESM 1 (DOCX 1916 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Divya Nair
    • 1
  • Muralidharan Vanuopadath
    • 1
  • Akshaya Balasubramanian
    • 1
  • Akshay Iyer
    • 1
  • Shankar Ganesh
    • 1
  • Abhishek Nair Anil
    • 1
  • Vijesh Vikraman
    • 1
  • Prasanth Pillai
    • 1
  • Chinchu Bose
    • 1
  • Bipin Gopalakrishnan Nair
    • 1
  • Jayashree Gopalakrishna Pai
    • 1
  • Sudarslal Sadasivan Nair
    • 1
    Email author
  1. 1.School of BiotechnologyAmrita Vishwa Vidyapeetham, AmritapuriKollamIndia

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