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Comparison of β-carotene and Spirulina (Arthrospira platensis) in mullet (Mugil liza) diets and effects on antioxidant performance and fillet colouration

  • Victor Torres Rosas
  • José María Monserrat
  • Martin Bessonart
  • Larisa Magnone
  • Luis Alberto Romano
  • Marcelo Borges TesserEmail author
Article

Abstract

The addition of pure carotenoids is a common practice in some aquaculture species production since its use in fish feed is known to improve carcass and antioxidant capacity. Spirulina (Arthrospira) cyanobacteria are a rich source of carotenoids that has proven health benefits for fish welfare. In the current experiment, four isonitrogenous (38%) isolipidic (9%) diets were made, including a control diet (free of β-carotene and Spirulina, SP0), β-carotene diet (50 mg kg−1, β0), and two Spirulina (Arthrospira platensis) diets with 2% (SP2) and 4% (SP4) of total feed inclusion. The experimental diets were given to juvenile mullets in a controlled recirculation system. The parameters evaluated were growth, colour, total carotene content of muscle, and antioxidant capacity of the liver and muscle. Significant differences were found (p < 0.05) in the final weight, specific growth rate, and feed conversion ratio between the SP0 treatment and the β0, SP2, and SP4 treatments. The colorimetric analysis showed that the redness and carotene deposition in muscle of mullet is statistically the same between β0 and SP4. Mullets fed with Spirulina (SP2 and SP4 treatments) had a statistical higher antioxidant capacity against radical peroxyls in liver tissue, showing lower lipid peroxidation (TBARS). In conclusion, Spirulina can be a suitable substitute for pure β-carotene addition in fish diets, improving some of the health benefits from this carotene.

Keywords

Antioxidant capacity Colorimetric analysis Fish TBARS 

Notes

Funding information

Part of this study was supported by a grant from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq–Universal, process number 404075/2016-9 and CNPq, process number 408921/2013-7- Prospecção de 245 substâncias antioxidantes de organismos marinhos–SAOMAR) and by Capes (Ciências do Mar). Monserrat, J.M., Romano, L.A., and Tesser, M.B. are research fellows from the Brazilian agency CNPq.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All experimental procedures involving fish were performed in accordance with the guidelines and approved by the Ethics Committee (FURG- CEUA Pq036/2014).

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Instituto de Oceanografia (IO)EMA, Universidade Federal do Rio Grande-FURGRio GrandeBrazil
  2. 2.Laboratório de Nutrição de Organismos Aquáticos (LANOA), IORio GrandeBrazil
  3. 3.Instituto de Ciências Biológicas (ICB)Universidade Federal do Rio Grande–FURGRio GrandeBrazil
  4. 4.Laboratório de Bioquímica Funcional de Organismos Aquáticos (BIFOA), IORio GrandeBrazil
  5. 5.Laboratorio de Recursos Naturales Facultad de Ciencias–UDeLaRMontevideoUruguay
  6. 6.Estación Experimental de Investigaciones Marinas y Acuicultura, DINARACabo PolonioUruguay
  7. 7.Laboratório de Patologia e Imunologia de Organismos AquáticosInstituto de OceanografiaRio GrandeBrazil

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