Strategic mixed substrate cultivation of microalgae: productivity, respiration, yield, and lipid quality
Mixotrophic cultivation of three microalgae species, Chlorella sorokiniana, Scenedesmus obliquus and Scenedesmus abundans, was carried out with a mixed substrate containing glucose and acetate (1:1) under indoor and outdoor conditions. For indoor cultivation, the highest biomass productivities for C. sorokiniana, S. obliquus and C. abundans were 331 ± 11.5, 317 ± 3.5, and 130.7 ± 3.1 mg L−1 day−1, respectively. The key result showed that the highest lipid productivities (29.8–36.2 mg L−1 day−1) were obtained for C. sorokiniana for the cases in which acetate was added on day 1 in glucose-mediated cultures. Lipid accumulation was higher (27–36% of dry weight) for S. obliquus and S. abundans. The highest amount of lipid was accumulated for the strategies in which acetate was added on day 1 in glucose-mediated culture. C. sorokiniana showed the highest biomass yields of 0.39 g g−1 as compared to the other two species. For outdoor cultivation, the biomass and lipid productivity values for C. sorokiniana and S. obliquus were 172.8 ± 4.2 and 183.9 ± 6.8 mg L−1 day−1 and 30.5 ± 2.2 and 26.9 ± 3.1 mg L−1 day−1, respectively.
KeywordsMixotrophic cultivation Chlorophyta Biodiesel Respiratory loss Biomass loss Outdoor cultivation
Ms. Suvidha Gupta is thankful to the Council of Scientific and Industrial Research (CSIR), New Delhi for providing Senior Research Fellowship (SRF) for her Ph.D. work.
The corresponding author was financially supported by the Department of Science and Technology (DST), New Delhi, under the scheme of DST INSPIRE Faculty Award (IFA13–ENG63).
- Bischoff HW, Bold HC (1963) Some soil algae from enchanted rock and related algal species. Phycological Studies, University of Texas 4:1–95Google Scholar
- Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Physiol Pharmacol 37:911–917Google Scholar
- Gupta S, Pandey RA, Pawar SB (2017) Bioremediation of synthetic high strength COD wastewater using microalgae sp. Chlorella pyrenoidosa. Bioremediat J 21:38–51Google Scholar
- Hegewald E (2000) New combinations in the genus Desmodesmus (Chlorophyceae, Scenedesmaceae). Algol Stud 96:1–18Google Scholar
- Li Y, Zhou W, Hu B, Min M, Chen P, Ruan RR (2012) Effect of light intensity on algal biomass accumulation and biodiesel production for mixotrophic strains Chlorella kessleri and Chlorella protothecoide cultivated in highly concentrated municipal wastewater. Biotechnol Bioeng 109:2222–2229CrossRefGoogle Scholar
- Pawar S, Gupta S (2018) Mass production of microalgae in photobioreactors for biodiesel application: selection, limitations, and optimization. In: Purohit H, Kalia V, Vaidya A, Khardenavis A (eds) Optimization and applicability of bioprocesses. Springer, Singapore, pp 211–232Google Scholar
- Sharma AK, Sahoo PK, Singhal S, Patel A (2016) Impact of various media and organic carbon sources on biofuel production potential from Chlorella spp. 3 Biotech 6:116Google Scholar