A novel cell lysis system induced by phosphate deficiency in the cyanobacterium Synechocystis sp. PCC 6803
In the cultivation of microalgae for the production of useful compounds, cell disruption to extract the products of interest is a bottleneck process. To establish a cost-effective method to recover these cellular compounds, we developed a method to induce cell lysis via phosphate deficiency in the cyanobacterium Synechocystis sp. PCC 6803. In this system, the promoter from the phoA gene for alkaline phosphatase expressed bacteriophage genes encoding the lytic enzymes holin and endolysin, thus the cell lysis is induced under phosphate-deficient condition. We observed that 90% of the cells, introduced this bacteriophage genes, were lysed after 24 h of incubation under phosphate-deficient conditions. We also developed a method to induce cell lysis in highly concentrated cells for the efficient recovery of valuable cellular products and observed over 90% cell lysis after 16 h of incubation under these conditions. This inducible lysis system may contribute to decreased cell disruption costs in the algal biotechnology industry.
KeywordsCell disruption Endolysin Extraction Holin Phosphate sensor
We are grateful to the National Institute of Technology and Evaluation (NITE) for providing purified DNA of the S. enterica phage P22.
This work was financially supported by JSPS KAKENHI Grant no. JP24119501.
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