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Life history, growth, and pigment content of two morphological variants of Hypnea pseudomusciformis (Gigartinales, Rhodophyta)

  • Fábio Nauer
  • Lígia Ayres-Ostrock
  • Ana Maria Amorim
  • Janaína Pires Santos
  • Fungyi Chow
  • Estela M. Plastino
  • Mariana C. Oliveira
Article

Abstract

Specimens previously described as Hypnea musciformis and Hypnea nigrescens in Brazil were shown to be morphological variants of a new species named Hypnea pseudomusciformis, which has been used for the production of carrageenan on the coast of Brazil. In this study, individuals of both morphological variants were collected in the field and cultivated in unialgal culture conditions. The life history of both variants was completed in 118 days. Tetrasporophytes produced tetrasporangia in 40 days. Released tetraspores germinated and 15 days later, an erect cylindrical axis developed from basal disk. At the age of 35 days, spermatangial conceptacles could be observed, and after 3 days of co-culture of male and female gametophytes, cystocarps could be observed, indicating the occurrence of fertilization. Finally, carpospores were released from cystocarps after 10 days, germinated and originated new tetrasporophytes in 15 days. When cultured, the morphological differences between the “musciformis” and “nigrescens” variants were attenuated and the life histories were the same. Furthermore, both morphological variants had different growth rates, but similar pigment content. These results corroborate that, for Brazil, specimens previously identified as H. musciformis and H. nigrescens are variants of H. pseudomusciformis, a highly plastic species.

Keywords

Rhodophyta Hypnea Culture Growth rates Physiological plasticity Pigments 

Notes

Acknowledgements

Thanks to all members from Marine Algae Laboratories (LAM) from University of São Paulo, Brazil, and to Rosário Petti and William Oliveira for technical support. Special thanks to Dr. Nair S. Yokoya, from São Paulo Institute of Botany for the help in the early stages of culture.

Funding information

We are grateful for the support from FAPESP (Biota 2013/11833-3) and CNPq (301491/2013-5; 300148/ 93-3). This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.

Supplementary material

10811_2018_1630_MOESM1_ESM.docx (17 kb)
Table 1S Pigments content for all life phases for the two morphological variants of H. pseudomusciformis. (DOCX 16 kb)

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Department of Botany, Biosciences InstituteSão Paulo UniversitySão PauloBrazil

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