Responses of lipoxygenase, jasmonic acid, and salicylic acid to temperature and exogenous phytohormone treatments in Gracilariopsis lemaneiformis (Rhodophyta)
Jasmonates (jasmonic acid (JA) and methyl jasmonate (MJ)) and salicylic acid (SA) play roles in the growth and developmental processes of plants and the defense response against adverse stresses as phytohormone signals. To investigate their roles in algae, we analyzed the sequences of two genes encoding lipoxygenase (LOX) and their expression profiles in response to temperature and phytohormone treatments in the macroalga Gracilariopsis lemaneiformis (Rhodophyta). The two lox sequences from Gp. lemaneiformis (Gllox1 and Gllox2) shared only 23.90% identity. The following results were observed: (1) high temperature (33 °C) strongly stimulated Gllox2 mRNA level, LOX activity, and endogenous JA and SA contents; (2) exogenous MJ promoted Gllox2 expression (4.91-fold at 6 h), LOX activity (2.44-fold at 6 h), and JA level at normal temperature (23 °C); however, it only slightly increased Gllox2 expression and internal JA content and inhibited LOX activity at 33 °C; (3) similarly, SA induced Gllox2 expression (2.32-fold at 12 h), LOX activity (approximately 2.00-fold at 6 and 12 h) and SA level at 23 °C, but it mainly decreased these parameters at 33 °C; and (4) the addition of MJ had either no effect or an inhibitory effect on endogenous SA content, and analogous effects of exogenous SA on the endogenous JA content were observed at both temperatures. From these results we conclude that lipoxygenase positively participated in the responses to high temperature and exogenous MJ or SA stimuli, and meanwhile, exogenous MJ or SA exerted a promoting effect on its corresponding endogenous phytohormone accumulation.
KeywordsRhodophyta Gracilariopsis lemaneiformis Lipoxygenase Methyl jasmonate Jasmonic acid Salicylic acid Temperature stress
This work was supported by the National Natural Science Foundation of China (31672674, 41376151). This research was also sponsored by the K. C. Wong Magna Fund in Ningbo University.
- Babenko LM, Kosakivska IV, Akimov YA, Klymchuk DO, Skaternya TD (2014) Effect of temperature stresses on pigment content, lipoxygenase activity and cell ultrastructure of winter wheat seedlings. Genet Plant Physiol 4:117–125Google Scholar
- Cai X, Shao M, Sun X, Xu N (2011) Detection of multiple phytohormones by GC-MS technique in Gracilaria lemaneiformis and the response to nitrogen stresses. Oceanol Limnol Sinica 42:753–758 (in Chinese)Google Scholar
- Huang SW, Li R, Zhang Z, Li L, Gu X, Fan W, Lucas WJ, Wang X, Xie B, Ni P, Ren Y, Zhu H, Li J, Lin K, Jin W, Fei Z, Li G, Staub J, Kilian A, van der Vossen EAG, Wu Y, Guo J, He J, Jia Z, Ren Y, Tian G, Lu Y, Ruan J, Qian W, Wang M, Huang Q, Li B, Xuan Z, Cao J, Asan, Wu Z, Zhang J, Cai Q, Bai Y, Zhao B, Han Y, Li Y, Li X, Wang S, Shi Q, Liu S, Cho WK, Kim JY, Xu Y, Heller-Uszynska K, Miao H, Cheng Z, Zhang S, Wu J, Yang Y, Kang H, Li M, Liang H, Ren X, Shi Z, Wen M, Jian M, Yang H, Zhang G, Yang Z, Chen R, Liu S, Li J, Ma L, Liu H, Zhou Y, Zhao J, Fang X, Li G, Fang L, Li Y, Liu D, Zheng H, Zhang Y, Qin N, Li Z, Yang G, Yang S, Bolund L, Kristiansen K, Zheng H, Li S, Zhang X, Yang H, Wang J, Sun R, Zhang B, Jiang S, Wang J, du Y, Li S (2009) The genome of the cucumber, Cucumis sativus L. Nat Genet 41:1275–1281CrossRefPubMedGoogle Scholar
- Król P, Igielski R, Pollmann S, Kępczyńska E (2015) Priming of seeds with methyl jasmonate induced resistance to hemi-biotroph Fusarium oxysporum f. sp. lycopersici in tomato via 12-oxo-phytodienoic acid, salicylic acid, and flavonol accumulation. J Plant Physiol 179:122–132CrossRefPubMedGoogle Scholar
- Rakhmankulova ZF, Fedyaev VV, Rakhmatulina SR, Ivanov CP, Gilvanova IR, Usmanov IY (2010) The effect of wheat seed presowing treatment with salicylic acid on its endogenous content, activities of respiratory pathways, and plant antioxidant status. Russ J Plant Physiol 57:778–783CrossRefGoogle Scholar
- Salzman RA, Brady JA, Finlayson SA, Buchanan CD, Summer EJ, Sun F, Klein PE, Klein RR, Pratt LH, Cordonnier-Pratt MM, Mullet JE (2005) Transcriptional profiling of Sorghum induced by methyl jasmonate, salicylic acid, and aminocyclopropane carboxylic acid reveals cooperative regulation and novel gene responses. Plant Physiol 138:352–368CrossRefPubMedPubMedCentralGoogle Scholar
- Talieva MN, Kondrat'eva VV (2002) Influence of exogenous salicylic acid on the level of phytohormones in tissues of Phlox paniculata and Phlox setacea leaves with special reference to resistance against the powdery mildew causative agent Erysiphe cichoracearum DC. f. phlogis Jacz. Biol Bull 29:551–554CrossRefGoogle Scholar
- Vick BA, Zimmerman DC (1987) 3-Oxidative systems for modification of fatty acids: the lipoxygenase pathway. Lipids Struct Funct 9:53–90Google Scholar
- Yang X, Song J, Zhang Z (2010) Effect of high temperature on gene expression, volatile biosynthesis and postharvest quality of banana fruit during ripening and senescence. Ashs Conference 45:S143–S144Google Scholar