Oxygenic and anoxygenic photosynthesis in a sewage pond
Leachate sewage ponds at Phuket Integrated Waste Management (Phuket, Thailand) are typical hypereutrophic red-water ponds found at sewage treatment plants and piggery, feedlot and poultry waste ponds with mixed communities of anoxygenic purple photosynthetic bacteria (PPB) (Bacteriochlorophyll a) and Chlorella-type green algae (Chl a + b). In vivo integrating sphere spectrometer scans (Model A&E-S90-2D, A&E Lab (UK)) showed absorbance maxima at 678–680 nm (in vivo Chl a) and a double peak at 802 and 844 nm (in vivo BChl a). High Na2S (8.3 mol m−3) added to PM media selected for the PPB whereas Chlorella overwhelmed PPB in PM medium without high H2S. Photosynthetic electron transport rate (ETR) was measured using a blue-diode-based Junior PAM (Pulse Amplitude Modulation Fluorometer) on sewage pond leachate filtered onto glass fibre disks. Diuron herbicide resistance experiments allowed measurement of both oxygenic and anoxygenic photosynthesis of a mixed population of oxygenic and anoxygenic organisms to be estimated only in special circumstances. In separate culture, the ETR vs. E curves were Chlorella-type algae, Eopt ≈ 191 ± 10 μmol quanta m−2 s−1, ETRmax = 184 ± 6.7 μmol e− g−1 Chl a s−1; PPB, Eopt = 386 ± 15 μmol quanta m−2 s−1, ETRmax = 316 ± 7.3 μmol e− g−1 BChl a s−1 but in a mixture of Chlorella and PPB only the oxygenic photosynthesis could be detected. In sewage pond samples, PAM rapid light curves in the presence and absence of DCMU allowed separate estimates of oxygen and anoxygenic photosynthesis to be made only if the Chl a content was very low (Chl a ≈ 0.26 μg mL−1; BChl a ≈ 1.4 μg mL−1). If substantial amounts of Chl a were present, fluorescence from PSII overwhelmed the signal from RC-2 of PPB, preventing the detection of anoxygenic photosynthesis. New PAM technology to measure Chl a and BChl a fluorescence separately is needed.
KeywordsSewage leachate pond Oxygenic photosynthesis Anoxygenic photosynthesis Integrating sphere spectrophotometry PAM fluorometry
The author wishes to thank Prince Songkla University-Phuket for providing facilities for the project. The project was partially funded by the Faculty of Technology and Environmental Science, Prince Songkla University-Phuket. The co-operation of Phuket Integrated Waste Management (Wichit Sub-district, Mueang Phuket, District, Phuket 83000, Thailand) in encouraging this study and allowing us to collect sewage pond water samples is gratefully acknowledged.
- Cochran WG, Snedecor GW (1989) Statistical methods, 8th edn. Iowa State University Press, AmesGoogle Scholar
- Falkowski PG, Raven JA (2007) Aquatic photosynthesis, 2nd edn. Princeton University Press, PrincetonGoogle Scholar
- Gitelson A, Stark R, Oron G, Dor I (1997) Monitoring of polluted water bodies by remote sensing. In: Remote sensing and geographic information systems for design and operation of water resources systems (Proceedings of Rabat Symposium S3, April 1997). IAHS Publ 242:181–188Google Scholar
- Kim MK, Harwood CS (1991) Regulation of benzoate-CoA ligase in Rhodopseudomonas palustris. FEMS Microbiol Lett 83:199–203Google Scholar
- Larimer FW, Chain P, Hauser L, Lamerdin J, Malfatti S, Do L, Land ML, Pelletier DA, Beatty JT, Lang AS, Tabita FR, Gibson JL, Hanson TE, Bobst C, Torres JLT, Peres C, Harrison FH, Gibson J, Harwood CS (2004) Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris. Nat Biotechnol 22:55–61CrossRefPubMedGoogle Scholar
- Nicholls HW (1973) Culture media-freshwater. In: Stein JR (ed) Handbook of phycological methods: culture methods and growth measurements, vol 1. Cambridge University Press, Cambridge, pp 7–24Google Scholar
- Papineau D, Walker JJ, Mojzsis SJ, Pace NR (2005) Composition and structure of microbial communities from stromatolites of Hamelin Pool in Shark Bay, Western Australia. Appl Environ Microbiol 2005:4822–4832Google Scholar
- Quinnell R, Howell D, Ritchie RJ (2017) Photosynthesis of an epiphytic resurrection fern Davallia angustata (Wall, ex Hook. & Grev.) Aust J Bot 65:348–356Google Scholar
- Ritchie RJ (2015) Photosynthetic light curve fitting models. Available at www.researchgate.net/publication/283052624_Photosynthetic_Light_Curve_Fitting_Models. [Verified 26 May 2016]
- Ritchie RJ (2018) Measurement of chlorophylls a and b and bacteriochlorophyll a in organisms from hypereutrophic auxinic waters. J Appl Phycol. https://doi.org/10.1007/s10811-018-1431-4
- Ritchie RJ, Larkum AWD, Ribas A (2017) Could photosynthesis function on Proxima Centauri b? Int J Astrobiol:1–30Google Scholar