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Journal of Applied Phycology

, Volume 30, Issue 3, pp 1795–1806 | Cite as

Establishment of a new strategy against Microcystis bloom using newly isolated lytic and toxin-degrading bacteria

  • Changsu Lee
  • Min Seo Jeon
  • Thi-Thao Vo
  • Chulhwan Park
  • Jong-Soon Choi
  • Joseph Kwon
  • Seong Woon Roh
  • Yoon-E Choi
Article

Abstract

Unwanted, rapid increases in the algal populations of water systems cause harmful algal blooms, which have recently become a major environmental problem. The cyanobacterium Microcystis aeruginosa is the most prevalent bloom species and is responsible for the majority of blooms in freshwater environments. In this study, we attempted to develop an eco-friendly method to suppress M. aeruginosa bloom based on a biological control using bacteria newly isolated from the soil. In a screen for bacteria with strong lethal activity toward Microcystis, we isolated Bacillus sp. T4 and characterised its algicidal activity. Microcystis aeruginosa cells were killed via indirect attack by compound(s) secreted by T4 bacteria. ELISA revealed a dramatic increase in extracellular microcystins in M. aeruginosa cultures upon treatment with T4. Therefore, we screened for bacteria that could degrade these toxins, and three new isolates (R12, S42 and S65) were identified. Simultaneous application of both T4 as a lytic agent and R12 or S42 as toxin-degrading bacteria could eliminate both Microcystis cells and its problematic toxin. Our eco-friendly approach, based on the application of newly isolated bacteria, provides a novel method to control harmful algal blooms.

Keywords

Algal bloom Cyanobacterium Bacterial isolation Microcystis Biological control Microcystin degradation 

Notes

Acknowledgements

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (2016R1D1A1B03932773), the World Institute of Kimchi (KE1802-2), funded by the Ministry of Science and ICT and Korea Basic Science Institute under the R&D program (Project No. C38703) supervised by the Ministry of Science and ICT, Republic of Korea. This work was also supported by the research program of KAERI, Republic of Korea. In addition, this research was supported by Korea University Future Research Grant and Korea University (OJERI) Grant.

Conflict of interest statement

The authors declare no conflict of interest.

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Changsu Lee
    • 1
  • Min Seo Jeon
    • 2
  • Thi-Thao Vo
    • 3
  • Chulhwan Park
    • 4
  • Jong-Soon Choi
    • 5
  • Joseph Kwon
    • 5
  • Seong Woon Roh
    • 1
  • Yoon-E Choi
    • 2
  1. 1.Microbiology and Functionality Research Group, World Institute of KimchiGwangjuRepublic of Korea
  2. 2.Division of Environmental Science and Ecological EngineeringCollege of Life Sciences and Biotechnology, Korea UniversitySeoulRepublic of Korea
  3. 3.Department of Bioactive Material SciencesChonbuk National UniversityJeonju-siRepublic of Korea
  4. 4.Department of Chemical EngineeringKwangwoon UniversitySeoulRepublic of Korea
  5. 5.Biological Disaster Analysis Group, Korea Basic Science InstituteDaejeonRepublic of Korea

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