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Journal of Applied Phycology

, Volume 30, Issue 5, pp 2803–2814 | Cite as

The red microalga Flintiella sanguinaria as a new exopolysaccharide producer

  • Clément Gaignard
  • Vanessa Macao
  • Christine Gardarin
  • Christophe Rihouey
  • Luc Picton
  • Philippe Michaud
  • Céline LarocheEmail author
6th Congress of the International Society for Applied Phycology

Abstract

Flintiella sanguinaria is a red unicellular microalgae belonging to Porphyridiophyceae with a high potential as an exopolysaccharide producer. A new culture medium was developed by a stoichiometry approach based on elemental analysis of microalgae, and culture conditions were improved after measurements of photosynthetic activity as a function of irradiance, temperature and pH. These optimal conditions were thus validated in a photobioreactor for the production of biomass and exopolysaccharide (EPS). After extraction and purification of the EPS, it was characterised by HPAEC-PAD, HPLC and FT-IR as a methylated and acetylated galactoxylan including a significant amount of rhamnose and glucuronic acid in its structure. Macromolecular conformation in dilute solution of native, deproteinised and desubstituted EPS from F. sanguinaria revealed that this polysaccharide had a strong associative behaviour in which hydrophobic interactions or hydrogen bonding but also proteins were implicated.

Keywords

Flintiella Rhodophyta Exopolysaccharide Culture Characterisation 

Notes

Funding information

This work was supported by the ANR POLYSALGUE project, grant ANR-15-CE21-0013 of the French Agence Nationale de la Recherche.

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Université Clermont Auvergne, CNRS, Institut PascalClermont-FerrandFrance
  2. 2.UNIROUEN, INSA Rouen, CNRS, PBSNormandie UnivRouenFrance

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