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Journal of Applied Phycology

, Volume 30, Issue 3, pp 1607–1616 | Cite as

Effects of UV-C radiation on Chlorella vulgaris, a biofilm-forming alga

  • Stéphane Pfendler
  • Badr Alaoui-Sossé
  • Laurence Alaoui-Sossé
  • Faisl Bousta
  • Lotfi Aleya
Article

Abstract

Photosynthetic biofilms proliferating on heritage monuments represent a major threat for curators leading to biodegradation and esthetic issues. Previous studies demonstrated that UV-C, used as a tool for biofilm eradication, is a promising avenue to combat microbial proliferation. In this study, this environmentally friendly method was tested on biofilm-forming Chlorella vulgaris suspension. Algal physiological response to UV-C was then assessed. Results showed that > 10 kJ m−2 UV-C exposure was enough to directly kill cells whereas low UV-C exposure reduced quantum yield of photosystem II and inhibited both respiration and photosynthesis. Clear relationships between UV-C exposure times and physiological responses were found. In addition, the use of VIS-light after UV-C treatment enhances chlorophyll bleaching. Our findings contribute to a better understanding of the physiological responses of Chlorella vulgaris to UV-C radiation allowing thus an optimization of the UV-C treatment reported in our previous studies.

Keywords

UV-C Chlorella Photosystem II Bleaching Biofilm 

Notes

Acknowledgements

We thank the Ministère de la Culture et de la Communication (France), the Laboratoire de Recherche des Monuments Historiques (LRMH, Paris), and the Conseil Régional of Franche-Comté for their financial aid. We express our appreciation to the editor and to the anonymous reviewers for helping to improve our paper.

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Stéphane Pfendler
    • 1
  • Badr Alaoui-Sossé
    • 1
  • Laurence Alaoui-Sossé
    • 1
  • Faisl Bousta
    • 2
  • Lotfi Aleya
    • 1
  1. 1.Laboratoire Chrono-Environnement—UMR 6249Université de Bourgogne Franche-ComtéBesançonFrance
  2. 2.Centre de Recherche sur la Conservation—Laboratoire de recherche des monuments historiques—USR 3224ParisFrance

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