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Journal of Applied Phycology

, Volume 29, Issue 5, pp 2503–2511 | Cite as

Different extraction procedures and analysis of protein from Ulva sp. in Brittany, France

  • Isuru Wijesekara
  • Marie Lang
  • Christel Marty
  • Marin-Pierre Gemin
  • Romain Boulho
  • Philippe Douzenel
  • Indira Wickramasinghe
  • Gilles Bedoux
  • Nathalie Bourgougnon
22ND INTERNATIONAL SEAWEED SYMPOSIUM, COPENHAGEN

Abstract

Seaweeds are well recognized as a potential protein source. The edible green seaweed, Ulva sp., is abundant in the Brittany Coast, France. This study examined the extraction of proteins and glycoproteins from this seaweed. Four different extraction procedures (Procedure 1: deionized water, DW; Procedure 2: lysis solution 1 (LS1) containing 8 M urea, 2% Tween 20, 2% Triton X-100, 30 mM dithiothreitol, and 1% polyvinylpyrolidine; Procedure 3: lysis solution 2 (LS2) containing 50 mM Tris-HCl buffer pH 8, 10 mM EDTA, 2 mM Na2S2O5, and 1% Triton X-100; Procedure 4: 50 mM Tris-HCl buffer, pH 8) were applied to extract proteins from Ulva sp. in Brittany. The protein contents (%, dry basis) in the above extracts from Procedures 1–4 were 4.36 ± 0.21, 11.88 ± 0.23, 10.34 ± 0.35, and 3.58 ± 0.48, respectively. Moreover, electrophoresis (SDS-PAGE) revealed that the protein profile varies with season. Three glycoprotein-rich fractions, namely, GP-1 (from Procedure GP1), GP-2-DA, and GP-2-DS (from Procedure GP2), were extracted from Ulva sp. GP-1 and GP-2-DA fractions have a higher protein content than neutral sugars, while GP-2-DS contains a higher amount of neutral sugars than proteins. Matrix-assisted laser desorption ionization-time of flight/mass spectrometry (MALDI-TOF/MS) technique was applied to further proteomic analysis of each glycoprotein-rich fraction. GP-2-DS was hydrolyzed with protease enzyme to confirm the availability of proteins, and interestingly, the particular hydrolysate shows no original peaks in the MALDI-TOF/MS analysis. All three glycoprotein-rich fractions show no cytotoxicity in Vero cells at the tested concentration (500 mg dw mL−1). Collectively, these results revealed that the extractable protein content and protein profile of Ulva sp. differ according to the extraction liquid system and the season. The utilization and value addition of proliferative Ulva sp. in Brittany as a protein source is promising but needs to consider the seasonal change of the protein profile.

Keywords

Ulva sp. Seaweeds Seaweed proteins Glycoproteins Nutraceuticals 

Notes

Acknowledgements

Postdoctoral research fellowship to Dr. Isuru Wijesekara (IW) by Universite de Britagne-Sud is highly appreciated. This work has been awarded as one of the best poster pitches at the ISS 2016, Copenhagen, Denmark, and the authors wish to thank Prof. Susan Lovstad Holdt and the organizing committee. The technical support from Dr. Nolwenn Terme, Ms. Laure Taupin, and Dr. Anne-Sophie Burlot are gratefully acknowledged. Furthermore, IW is thankful to Prof. Sampath Amaratunge, Prof. Sudantha Liyanage, anonymous reviewers, and the editor.

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Isuru Wijesekara
    • 1
    • 2
  • Marie Lang
    • 1
  • Christel Marty
    • 1
  • Marin-Pierre Gemin
    • 1
  • Romain Boulho
    • 1
  • Philippe Douzenel
    • 1
  • Indira Wickramasinghe
    • 2
  • Gilles Bedoux
    • 1
  • Nathalie Bourgougnon
    • 1
  1. 1.Laboratory of Marine Biotechnology and Chemistry (LBCM)University of South Brittany (UBS), European Institute of Marine Studies (IEUM)VannesFrance
  2. 2.Department of Food Science & Technology, Faculty of Applied SciencesUniversity of Sri JayewardenepuraNugegodaSri Lanka

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