Journal of Applied Phycology

, Volume 30, Issue 1, pp 401–410 | Cite as

Carbon fixation properties of three alkalihalophilic microalgal strains under high alkalinity

  • Masatoshi KishiEmail author
  • Tatsuki Toda


Carbon dioxide (CO2) recovery with high alkalinity microalgal culture is expected to be an energy-efficient and environmentally friendly process. To increase the CO2 recovery efficiency, selection of rapidly growing alkalihalophilic microalgae is necessary. This study optimized the culture conditions of three species of alkalihalophilic microalgae, Arthrospira platensis, Dunaliella salina, and Euhalothece sp., and compared their CO2 fixation potential. Although D. salina tolerated relatively high dissolved inorganic carbon (DIC; 0.50 mol L−1), its carbon fixation rate was found to be slower than the other two species. The two cyanobacteria, A. platensis and Euhalothece sp., favored high pH (9.8–10) and high DIC (0.23–1.1 mol L−1). Euhalothece sp. grew in the highest alkalinity, resulting in the strongest pH buffer against acidification during CO2 absorption. However, the carbon fixation properties of A. platensis and Euhalothece sp. under the same light condition were found to be similar (33 and 35 mmol L−1 day−1). These results indicate that the carbon fixation potential per medium inorganic carbon was higher in A. platensis than in the others. Arthrospira platensis was found to be favorable in a CO2 recovery process unless extremely high pH stability is needed.


Algae Cyanobacteria Carbon dioxide Alkalinity Growth optimization 



This study was partially supported by Japan Science and Technology Agency (JST)/Japan International Cooperation Agency (JICA), Science and Technology Research Partnership for Sustainable Development (SATREPS). The authors would like to offer deep appreciation to Ms. Maki Kobayashi, Mr. Kenta Nagatsuka, Ms. Yumi Hosokawa, Ms. Hidemi Onouchi, Ms. Mako Tagawa, and Ms. Midori Goto for their support in sampling and analyzing the data.

Supplementary material

10811_2017_1226_MOESM1_ESM.pdf (23 kb)
ESM 1 (PDF 23 kb)


  1. Ben-Amotz A, Polle JEW, Subba Rao DV (eds) (2009) The alga Dunaliella: biodiversity, physiology, genomics and biotechnology. Science PublishersGoogle Scholar
  2. Borowitzka MA (1999) Commercial production of microalgae: ponds, tanks, tubes and fermenters. J Biotechnol 70:313–321CrossRefGoogle Scholar
  3. Chi Z, Elloy F, Xie Y, Hu Y, Chen S (2014) Selection of microalgae and cyanobacteria strains for bicarbonate-based integrated carbon capture and algae production system. Appl Biochem Biotechnol 172:447–457CrossRefPubMedGoogle Scholar
  4. Chi Z, O’Fallon JV, Chen S (2011) Bicarbonate produced from carbon capture for algae culture. Trends Biotechnol 29:537–541CrossRefPubMedGoogle Scholar
  5. Chi Z, Xie Y, Elloy F, Zheng Y, Hu Y, Chen S (2013) Bicarbonate-based integrated carbon capture and algae production system with alkalihalophilic cyanobacterium. Bioresour Technol 133:513–521CrossRefPubMedGoogle Scholar
  6. Gerasimenko LM, Mikhodyuk OS (2009) Halophilic algal-bacterial and cyanobacterial communities and their role in carbonate precipitation. Paleontol J 43:940–957CrossRefGoogle Scholar
  7. González-López CV, Acién Fernández FG, Fernández-Sevilla JM, Sánchez Fernández JF, Molina Grima E (2012) Development of a process for efficient use of CO2 from flue gases in the production of photosynthetic microorganisms. Biotechnol Bioeng 109:1637–1650CrossRefPubMedGoogle Scholar
  8. Gris B, Sforza E, Vecchiato L, Bertucco A (2014) Development of a process for an efficient exploitation of CO2 captured from flue gases as liquid carbonates for Chlorella protothecoides cultivation. Ind Eng Chem Res 53:16678–16688CrossRefGoogle Scholar
  9. Kang CD, Lee JS, Park TH, Sim SJ (2005) Comparison of heterotrophic and photoautotrophic induction on astaxanthin production by Haematococcus pluvialis. Appl Microbiol Biotechnol 68:237–241CrossRefPubMedGoogle Scholar
  10. Lee Y-K (1997) Commercial production of microalgae in the Asia-Pacific rim. J Appl Phycol 9:403–411CrossRefGoogle Scholar
  11. Lee Y-K, Chen W, Shen H, Han D, Li Y, Jones HDT, Timlin JA, Hu Q (2013) Basic culturing and analytical measurement techniques. In: Richmond A, Hu Q (eds) Handbook of microalgal culture: applied phycology and biotechnology, 2nd edn. John Wiley & Sons, Ltd., pp 37–68CrossRefGoogle Scholar
  12. Meier L, Pérez R, Azócar L, Rivas M, Jeison D (2015) Photosynthetic CO2 uptake by microalgae: an attractive tool for biogas upgrading. Biomass Bioenergy 73:102–109CrossRefGoogle Scholar
  13. Mikhodiuk OS, Gerasimenko LM, Akimov VN, Ivanovskiĭ RN, Zavarzin GA (2008) Ecophysiology and polymorphism of the unicellular extremely natronophilic cyanobacterium Euhalothece sp. Z-M001 from Lake Magadi. Mikrobiologiia 77:805–813PubMedGoogle Scholar
  14. NIST/SEMATECH (2015) e-Handbook of Statistical Methods. In: E-handb. Stat. Methods.
  15. Ogawa T, Terui G (1970) Studies on the growth of Spirulina platensis: (I) on the pure culture of Spirulina platensis. J Ferment Technol 48:361–367Google Scholar
  16. Pancha I, Chokshi K, Ghosh T, Paliwal C, Maurya R, Mishra S (2015) Bicarbonate supplementation enhanced biofuel production potential as well as nutritional stress mitigation in the microalgae Scenedesmus sp. CCNM 1077. Bioresour Technol 193:315–323CrossRefPubMedGoogle Scholar
  17. Radmer RJ, Parker BC (1994) Commercial applications of algae: opportunities and constraints. J Appl Phycol 6:93–98CrossRefGoogle Scholar
  18. Richardson K, Beardall J, Raven JA (1983) Adaption of unicellular algae to irradiance: an analysis of strategies. New Phytol 93:157–191CrossRefGoogle Scholar
  19. Rodrigues MS, Ferreira LS, Converti A, Sato S, Carvalho JCM (2010) Fed-batch cultivation of Arthrospira (Spirulina) platensis: potassium nitrate and ammonium chloride as simultaneous nitrogen sources. Bioresour Technol 101:4491–8. Doi:Google Scholar
  20. Rosenberg JN, Mathias A, Korth K, Betenbaugh MJ, Oyler GA (2011) Microalgal biomass production and carbon dioxide sequestration from an integrated ethanol biorefinery in Iowa: a technical appraisal and economic feasibility evaluation. Biomass Bioenergy 35:3865–3876CrossRefGoogle Scholar
  21. Rosenberg JN, Oyler GA, Wilkinson L, Betenbaugh MJ (2008) A green light for engineered algae: redirecting metabolism to fuel a biotechnology revolution. Curr Opin Biotechnol 19:430–436CrossRefPubMedGoogle Scholar
  22. Sathasivam R, Juntawong N, Program B (2013) Modified medium for enhanced growth of Dunaliella strains. Int J Curr Sci 5:67–73Google Scholar
  23. Silva CEDF, Gris B, Sforza E, Rocca NL, Bertucco A (2016) Effects of sodium bicarbonate on biomass and carbohydrate production in Synechococcus PCC 7002. Appl Biochem Biotechnol 49:241–246Google Scholar
  24. Spolaore P, Joannis-Cassan C, Duran E, Isambert A (2006) Commercial applications of microalgae. J Biosci Bioeng 101:87–96CrossRefPubMedGoogle Scholar
  25. Su CM, Hsueh HT, Chen HH, Chu H (2012) Effects of dissolved inorganic carbon and nutrient levels on carbon fixation and properties of Thermosynechococcus sp. in a continuous system. Chemosphere 88:706–711CrossRefPubMedGoogle Scholar
  26. Toledo-Cervantes A, Serejo ML, Blanco S, Pérez R, Lebrero R, Muñoz R (2016) Photosynthetic biogas upgrading to bio-methane: boosting nutrient recovery via biomass productivity control. Algal Res 17:46–52CrossRefGoogle Scholar
  27. Tripathi R, Singh J, Thakur IS (2015) Characterization of microalga Scenedesmus sp. ISTGA1 for potential CO2 sequestration and biodiesel production. Renew Energy 74:774–781CrossRefGoogle Scholar
  28. Vonshak A (ed) (1997) Spirulina platensis (Arthrospira): physiology, cell-biology and biotechnology. Taylor & Francis, LondonGoogle Scholar
  29. Walsh BJ, Rydzak F, Palazzo A, Kraxner F, Herrero M, Schenk PM, Ciais P, Janssens IA, Peñuelas J, Niederl-Schmidinger A, Obersteiner M (2015) New feed sources key to ambitious climate targets. Carbon Balance Manag 10:26. doi: 10.1186/s13021-015-0040-7 CrossRefPubMedPubMedCentralGoogle Scholar
  30. White DA, Pagarette A, Rooks P, Ali ST (2013) The effect of sodium bicarbonate supplementation on growth and biochemical composition of marine microalgae cultures. J Appl Phycol 25:153–165CrossRefGoogle Scholar
  31. Yeh KL, Chang JS, Chen WM (2010) Effect of light supply and carbon source on cell growth and cellular composition of a newly isolated microalga Chlorella vulgaris ESP-31. Eng Life Sci 10:201–208CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  1. 1.Graduate School of EngineeringSoka UniversityTokyoJapan

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