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Journal of Applied Phycology

, Volume 29, Issue 3, pp 1729–1733 | Cite as

Development and characterization of simple sequence repeat markers for genetic analyses of Sargassum horneri (Sargassaceae, Phaeophyta) populations in Kyoto, Japan

  • Nakao KuboEmail author
  • Akio Douke
  • Tomokazu Nishigaki
  • Gento Tsuji
Article

Abstract

Simple sequence repeat (SSR) markers were developed for the brown alga Sargassum horneri (also known as “akamoku”) from an SSR-enriched genomic library. Of 22 SSR markers developed, 10 primer pairs produced clearly distinguishable DNA bands, indicating polymorphisms in S. horneri populations collected around Wakasa Bay, Kyoto, Japan. Ten primer pairs among the newly developed SSRs were also applicable to any of five other Sargassum species tested. To assess the utility of SSR markers for the classification of S. horneri, we genotyped 86 akamoku individuals from six populations (Naryu, Obase, Oshima, Satohami, Tai, and Taiza). We detected 148 alleles using 11 markers (10 new and 1 previously published). The allele number per locus ranged from 4 to 32 (average, 13.5). Based on the genotyping data for the 11 SSR markers, three of the six akamoku populations were separated on a phylogram. These results indicate that the SSR markers developed in this study are informative and useful for genetic analyses in Sargassum species.

Keywords

Akamoku DNA marker Microsatellite Sargassum Simple sequence repeat 

Notes

Acknowledgments

The authors thank Dr. S. Uwai for the helpful information for the marker analysis in S. horneri and Ms. H. Kasaoka for the technical assistance. This work was funded by the Academic Contribution To Region (ACTR) grant (grant no. A11) from Kyoto Prefectural University to G.T.

Supplementary material

10811_2016_1041_MOESM1_ESM.pdf (113 kb)
Fig S1 (PDF 113 kb)
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Table S1 (PDF 59 kb)
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Table S2 (PDF 66 kb)
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Table S3 (PDF 63 kb)
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Table S4 (PDF 55 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Nakao Kubo
    • 1
    • 2
    Email author
  • Akio Douke
    • 3
  • Tomokazu Nishigaki
    • 3
    • 4
  • Gento Tsuji
    • 1
  1. 1.Graduate School of Life and Environmental SciencesKyoto Prefectural UniversityKyotoJapan
  2. 2.Biotechnology Research DepartmentKyoto Prefectural Agriculture, Forestry and Fisheries Technology CenterKyotoJapan
  3. 3.Fisheries Technology Department, Kyoto Prefectural AgricultureForestry and Fisheries Technology CenterKyotoJapan
  4. 4.Department of AgricultureForestry and Fisheries, Kyoto Prefectural OfficeKyotoJapan

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