Development and characterization of simple sequence repeat markers for genetic analyses of Sargassum horneri (Sargassaceae, Phaeophyta) populations in Kyoto, Japan
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Simple sequence repeat (SSR) markers were developed for the brown alga Sargassum horneri (also known as “akamoku”) from an SSR-enriched genomic library. Of 22 SSR markers developed, 10 primer pairs produced clearly distinguishable DNA bands, indicating polymorphisms in S. horneri populations collected around Wakasa Bay, Kyoto, Japan. Ten primer pairs among the newly developed SSRs were also applicable to any of five other Sargassum species tested. To assess the utility of SSR markers for the classification of S. horneri, we genotyped 86 akamoku individuals from six populations (Naryu, Obase, Oshima, Satohami, Tai, and Taiza). We detected 148 alleles using 11 markers (10 new and 1 previously published). The allele number per locus ranged from 4 to 32 (average, 13.5). Based on the genotyping data for the 11 SSR markers, three of the six akamoku populations were separated on a phylogram. These results indicate that the SSR markers developed in this study are informative and useful for genetic analyses in Sargassum species.
KeywordsAkamoku DNA marker Microsatellite Sargassum Simple sequence repeat
The authors thank Dr. S. Uwai for the helpful information for the marker analysis in S. horneri and Ms. H. Kasaoka for the technical assistance. This work was funded by the Academic Contribution To Region (ACTR) grant (grant no. A11) from Kyoto Prefectural University to G.T.
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