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Journal of Applied Phycology

, Volume 29, Issue 3, pp 1171–1178 | Cite as

Cultivation of Chlorella vulgaris with swine wastewater and potential for algal biodiesel production

  • Kibok Nam
  • Hansol Lee
  • Sung-Woon Heo
  • Yong Keun Chang
  • Jong-In Han
Article

Abstract

In this study, an alga-based simultaneous process of treating swine wastewater (SWW) and producing biodiesel was explored. Chlorella vulgaris (UTEX-265) was employed as a model species, and a SWW-based medium was prepared by dilution with tap water. Chlorella vulgaris grew well in the SWW-based medium, and at optimum dilution ratios, it exceeded the conventional culture medium in terms of biomass concentration and productivity. In eightfold diluted SWW, which supported the maximum growth, biomass productivity was 0.247 g L−1 day−1, while the productivity was merely 0.165 g L−1 day−1 in standard tris-acetate-phosphorous (TAP) algal medium. In addition, fatty acid methyl ester (FAME) productivity was greater in the SWW-based medium (0.067 versus 0.058 g L−1 day−1). This enhanced productivity resulted in more than 95 % removal of both nitrogen and phosphorous. All these show that C. vulgaris cultivation is indeed possible in a nutrient-rich wastewater with appropriate dilution, and in so doing, the wastewater can effectively be treated.

Keywords

Chlorella vulgaris Chlorophyceae Swine wastewater Nutrient removal Biofuel production 

Notes

Acknowledgments

This work was supported by the Advanced Biomass R&D Center (ABC) of the Global Frontier Project funded by the Ministry of Science, ICT, and Future Planning (ABC-2010-0029728).

References

  1. Abe K, Bito T, Sato A, Aburai N (2014) Effects of light intensity and magnesium supplementation in pretreatment cycle on ammonium removal from wastewater of photobioreactor using a biofilter composed of the aerial microalga Trentepohlia aurea. J Appl Phycol 26:341–347CrossRefGoogle Scholar
  2. American Public Health Association (1995) Standard methods for the examination of water and wastewater. American Public Health Association, American Water Works association, Water Environment Federation, WashingtonGoogle Scholar
  3. Baek K, Yang JW (2005) Humic-substance-enhanced ultrafiltration for removal of heavy metals. Separ Sci Technol 40:699–708CrossRefGoogle Scholar
  4. Bamgboye AI, Hansen AC (2008) Prediction of cetane number of biodiesel fuel from the fatty acid methyl ester (FAME) composition. Int Agrophys 22:21–29Google Scholar
  5. Bhatnagar A, Chinnasamy S, Singh M, Das KC (2011) Renewable biomass production by mixotrophic algae in the presence of various carbon sources and wastewaters. Appl Energ 88:3425–3431CrossRefGoogle Scholar
  6. Borowitzka MA, Moheimani NR (2013) Sustainable biofuels from algae. Mitig Adapt Strat Global Change 18:13–25CrossRefGoogle Scholar
  7. Chisti Y (2007) Biodiesel from microalgae. Biotechnol Adv 25:294–306CrossRefPubMedGoogle Scholar
  8. Chisti Y (2008a) Biodiesel from microalgae beats bioethanol. Trends Biotechnol 26:126–131CrossRefPubMedGoogle Scholar
  9. Chisti Y (2008b) Response to Reijnders: do biofuels from microalgae beat biofuels from terrestrial plants? Trends Biotechnol 26:351–352CrossRefGoogle Scholar
  10. Cho S, Luong TT, Lee D, Oh Y-K, Lee T (2011) Reuse of effluent water from a municipal wastewater treatment plant in microalgae cultivation for biofuel production. Bioresource Technol 102:8639–8645CrossRefGoogle Scholar
  11. Craggs RJ, Lundquist TJ, Benemann JR (2013) Wastewater treatment and algal biofuel production. In: Borowitzka MA, Moheimani NR (eds) Algae for biofuels and energy. Springer, Dordrecht, pp. 153–164CrossRefGoogle Scholar
  12. Fallowfield H, Garrett M (1985) The photosynthetic treatment of pig slurry in temperate climatic conditions: a pilot-plant study. Agr Wastes 12:111–136CrossRefGoogle Scholar
  13. Folch J, Lees M, Sloane-Stanley G (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509PubMedGoogle Scholar
  14. Genty B, Briantais J-M, Baker NR (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim Biphys Acta-Gen Subjects 990:87–92CrossRefGoogle Scholar
  15. Gonzalez C, Marciniak J, Villaverde S, Garcia-Encina PA, Munoz R (2008) Microalgae-based processes for the biodegradation of pretreated piggery wastewaters. Appl Microbiol Biot 80:891–898CrossRefGoogle Scholar
  16. Gopinath A, Puhan S, Nagarajan G (2009) Relating the cetane number of biodiesel fuels to their fatty acid composition: a critical study. P I Mech Eng D-J Aut 223 (D4):565–583Google Scholar
  17. Jia Q, Xiang W, Yang F, Hu Q, Tang M, Chen C, Wang G, Dai S, Wu H, Wu H (2016) Low-cost cultivation of Scenedesmus sp. with filtered anaerobically digested piggery wastewater: biofuel production and pollutant remediation. J Appl Phycol 28:727–736CrossRefGoogle Scholar
  18. Halfhide T, Åkerstrøm A, Lekang OI, Gislerød HR, Ergas SJ (2014) Production of algal biomass, chlorophyll, starch and lipids using aquaculture wastewater under axenic and non-axenic conditions. Algal Res 6 (B):152–159Google Scholar
  19. Kumar MS, Miao ZHH, Wyatt SK (2010) Influence of nutrient loads, feeding frequency and inoculum source on growth of Chlorella vulgaris in digested piggery effluent culture medium. Bioresource Technol 101:6012–6018CrossRefGoogle Scholar
  20. Li Y, Chen YF, Chen P, Min M, Zhou W, Martinez B, Zhu J, Ruan R (2011) Characterization of a microalga Chlorella sp. well adapted to highly concentrated municipal wastewater for nutrient removal and biodiesel production. Bioresource Technol 102:5138–5144CrossRefGoogle Scholar
  21. McGinn PJ, Dickinson KE, Park KC, Whitney CG, MacQuarrie SP, Black FJ, Frigon J-C, Guiot SR, O’Leary SJB (2012) Assessment of the bioenergy and bioremediation potentials of the microalga Scenedesmus sp. AMDD cultivated in municipal wastewater effluent in batch and continuous mode. Algal Res 1:155–165CrossRefGoogle Scholar
  22. Moon M, Kim CW, Farooq W, Suh WI, Shrivastav A, Park MS, Mishra SK, Yang JW (2014) Utilization of lipid extracted algal biomass and sugar factory wastewater for algal growth and lipid enhancement of Ettlia sp. Bioresource Technol 163:180–185CrossRefGoogle Scholar
  23. Nascimento IA, Marques SSI, Cabanelas ITD, de Carvalho GC, Nascimento MA, de Souza CO, Druzian JI, Hussain J, Liao W (2014) Microalgae versus land crops as feedstock for biodiesel: productivity, quality, and standard compliance. Bioenergy Res 7:1002–1013Google Scholar
  24. Nayak M, Karemore A, Sen R (2016) Performance evaluation of microalgae for concomitant wastewater bioremediation, CO biofixation and lipid biosynthesis for biodiesel application. Algal Res 16:216–223Google Scholar
  25. Nwoba EG, Ayre JM, Moheimani NR, Ubi BE, Ogbonna JC (2016) Growth comparison of microalgae in tubular photobioreactor and open pond for treating anaerobic digestion piggery effluent. Algal Res 17:268–276CrossRefGoogle Scholar
  26. Oswald WJ (1988) Micro-algae and waste-water treatment. In: Borowitzka MA, Borowitzka LJ (eds) Micro-algal biotechnology. Cambridge University Press, Cambridge, pp. 305–328Google Scholar
  27. Pires JCM, Alvim-Ferraz MCM, Martins FG, Simoes M (2012) Carbon dioxide capture from flue gases using microalgae: engineering aspects and biorefinery concept. Renew Sust Energ Rev 16:3043–3053CrossRefGoogle Scholar
  28. Pittman JK, Dean AP, Osundeko O (2011) The potential of sustainable algal biofuel production using wastewater resources. Bioresource Technol 102:17–25Google Scholar
  29. Ramos MJ, Fernández CM, Casas A, Rodríguez L, Pérez Á (2009) Influence of fatty acid composition of raw materials on biodiesel properties. Bioresource Technol 100:261–268CrossRefGoogle Scholar
  30. Ryu B-G, Kim J, Farooq W, Han J-I, Yang J-W, Kim W (2014a) Algal–bacterial process for the simultaneous detoxification of thiocyanate-containing wastewater and maximized lipid production under photoautotrophic/photoheterotrophic conditions. Bioresource Technol 162:70–79CrossRefGoogle Scholar
  31. Ryu B-G, Kim K, Kim J, Han J-I, Yang J-W (2013) Use of organic waste from the brewery industry for high-density cultivation of the docosahexaenoic acid-rich microalga, Aurantiochytrium sp. KRS101. Bioresource Technol 129:351–359CrossRefGoogle Scholar
  32. Ryu B-G, Park G-Y, Yang J-W, Baek K (2011) Electrolyte conditioning for electrokinetic remediation of as, Cu, and Pb-contaminated soil. Sep Purif Technol 79:170–176CrossRefGoogle Scholar
  33. Ryu BG, Kim J, Yoo G, Lim JT, Kim W, Han JI, Yang JW (2014b) Microalgae-mediated simultaneous treatment of toxic thiocyanate and production of biodiesel. Bioresource Technol 158:166–173CrossRefGoogle Scholar
  34. Seyfabadi J, Ramezanpour Z, Khoeyi ZA (2011) Protein, fatty acid, and pigment content of Chlorella vulgaris under different light regimes. J Appl Phycol 23:721–726CrossRefGoogle Scholar
  35. Silva A, Figueiredo SA, Sales MG, Delerue-Matos C (2009) Ecotoxicity tests using the green algae Chlorella vulgaris—a useful tool in hazardous effluents management. J Hazard Mater 167:179–185CrossRefPubMedGoogle Scholar
  36. Singh UB, Ahluwalia AS (2013) Microalgae: a promising tool for carbon sequestration. Mitig Adapt Strat Gl 18:73–95CrossRefGoogle Scholar
  37. Tam NFY, Wong YS (1996) Effect of ammonia concentrations on growth of Chlorella vulgaris and nitrogen removal from media. Bioresource Technol 57:45–50CrossRefGoogle Scholar
  38. Tigini V, Franchino M, Bona F, Varese GC (2016) Is digestate safe? A study on its ecotoxicity and environmental risk on a pig manure. Sci Total Environ 551:127–132CrossRefPubMedGoogle Scholar
  39. Wang HY, Xiong HR, Hui ZL, Zeng XB (2012) Mixotrophic cultivation of Chlorella pyrenoidosa with diluted primary piggery wastewater to produce lipids. Bioresource Technol 104:215–220CrossRefGoogle Scholar
  40. Wellburn AR (1994) The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol 144:307–313CrossRefGoogle Scholar
  41. Zhang L, Lu H, Zhang Y, Li B, Liu Z, Duan N, Liu M (2016) Nutrient recovery and biomass production by cultivating Chlorella vulgaris 1067 from four types of post-hydrothermal liquefaction wastewater. J Appl Phycol 28:1031–1039CrossRefGoogle Scholar
  42. Zhu L, Wang Z, Shu Q, Takala J, Hiltunen E, Feng P, Yuan Z (2013) Nutrient removal and biodiesel production by integration of freshwater algae cultivation with piggery wastewater treatment. Water Res 47:4294–4302CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  1. 1.Department of Chemical and Biomolecular EngineeringKAISTDaejeonRepublic of Korea
  2. 2.Advanced Biomass R&D CenterKAISTDaejeonRepublic of Korea
  3. 3.Department of Civil and Environmental EngineeringKAISTDaejeonRepublic of Korea

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