Journal of Applied Phycology

, Volume 29, Issue 2, pp 769–773 | Cite as

Therapeutic efficacy in BALB/C mice of extract from marine alga Canistrocarpus cervicornis (Phaeophyceae) against herpes simplex virus type 1

  • Caroline de Souza Barros
  • Valeria Garrido
  • Vanessa Melchiades
  • Rafaela Gomes
  • Max Willian Lisboa Gomes
  • Valeria Laneuville Teixeira
  • Izabel Christina Nunes de Palmer Paixão
V REDEALGAS WORKSHOP (Rio De Janeiro, Brazil)
First Online:
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Abstract

Studies with diterpenes from marine brown alga Canistrocarpus cervicornis showed the antiviral potential of the products from this alga in controlling the replication of HSV-1 and maintaining low cytotoxicity. Hence, the aim of this work was to evaluate the anti-herpetic efficacy of C. cervicornis extract ointment in BALB/c mice. To test the anti-herpetic efficacy in vivo, four groups of BALB/c mice (n = 5) were used: 1—untreated, 2—extract ointment (2 % or 0.4 mg cm−2 dose−1), 3—Acyclovir cream (5 % or 1.0 mg cm−2 dose−1), and 4—ointment base. The right midflank of each mouse was clipped and depilated with a chemical depilatory. After 2 days, the skin area was scratched and inoculated with HSV-1. The ointments and cream were applied three times a day over a 16-day period, beginning 1 h after virus inoculation. The development of skin lesions was continuously monitored and scored. To evaluate the effect of C. cervicornis topical treatment on biochemical parameters and on body weight, two uninfected groups were formed: an untreated group and a group treated with ointment C. cervicornis extract (2 %). The signs of infection appeared from the second day after infection, while on the 10th day of the experiment, the ointment base and untreated groups had significantly more severe lesions than did the groups that were treated with extract (p < 0.05) or acyclovir (p < 0,01). The topical application of extract ointment did not change body weight, hepatic, or renal function suggesting that the extract has a low toxicity in this route of administration. These results suggest that the extract may be useful in reducing the severity of HSV-1 cutaneous lesions.

Keywords

Canistrocarpus cervicornis Phaeophyceae HSV-1 Marine natural product Antiviral 
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Notes

Acknowledgments

The authors are grateful to CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) for the financial support and for Productivity Fellowships to ICNPP (303368/2013-6) and VLT (301420/2010-6). ICNPP (E-26/103.024/2011) and VLT (E-26/103.176/2011) also thank the FAPERJ (Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro) for the Cientista do Nosso Estado Fellowship. CSB thanks FAPERJ for the DSc fellowship (E-26/100.770/2012). MWLG thanks FAPERJ for the fellowship (E-26/200.712/2015).

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Caroline de Souza Barros
    • 1
  • Valeria Garrido
    • 1
  • Vanessa Melchiades
    • 1
  • Rafaela Gomes
    • 1
  • Max Willian Lisboa Gomes
    • 1
  • Valeria Laneuville Teixeira
    • 2
  • Izabel Christina Nunes de Palmer Paixão
    • 1
  1. 1.Laboratório de Virologia Molecular e Biotecnologia Marinha, Programa de Pós-graduação em Ciências e Biotecnologia, Departamento de Biologia Celular e Molecular, Instituto de BiologiaUniversidade Federal FluminenseNiteróiBrazil
  2. 2.Laboratório Algamar, Departamento de Biologia MarinhaInstituto de Biologia, Universidade Federal FluminenseNiteróiBrazil

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