Journal of Applied Phycology

, Volume 28, Issue 5, pp 2631–2640 | Cite as

Effect of high CO2 concentrations on the growth and macromolecular composition of a heat- and high-light-tolerant microalga

  • Prachi Varshney
  • Sujata Sohoni
  • Pramod P. Wangikar
  • John Beardall
Article

Abstract

A green microalga, Acutodesmus sp., a close relative of Acutodesmus deserticola, was isolated from the wastewater discharges of an oil refinery in India. This study examined the effects of light intensity, temperature, pH, and high-CO2 treatments (up to 20 %) on the growth of the alga and investigated the effects of different CO2 treatments on its macromolecular composition (protein, carbohydrate, and lipids). Under controlled laboratory conditions, the alga showed high growth rates over a wide range of light (up to 700 μmol photons m−2 s−1), temperature (up to 40 °C), and pH (5–10) conditions. In the stationary phase, the highest protein and carbohydrate content was found to be 71.52 and 40.72 % of dry weight at 5 and 15 % CO2, respectively. After 5 days of cultivation, the maximum dry weight biomass attained in these cultures was 1.149, 1.99, 1.75, and 1.65 g L−1 at 5, 10, 15, and 20 % CO2, respectively, indicating that this strain has significant tolerance to CO2. These results indicate that this strain is a promising candidate for use in biofixation of CO2 from the flue gases emitted by industries, and it also has a strong potential as a feedstock for value-added substances.

Keywords

Green microalga High-CO2 tolerance Industrial wastewater Light intensity Temperature 

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Prachi Varshney
    • 1
    • 2
    • 3
  • Sujata Sohoni
    • 2
    • 4
  • Pramod P. Wangikar
    • 2
    • 4
    • 5
  • John Beardall
    • 3
  1. 1.IITB-Monash Research Academy, CSE BuildingIndian Institute of Technology BombayMumbaiIndia
  2. 2.Department of Chemical EngineeringIndian Institute of Technology BombayMumbaiIndia
  3. 3.School of Biological SciencesMonash UniversityClaytonAustralia
  4. 4.DBT-Pan IIT Center for BioenergyIndian Institute of Technology BombayMumbaiIndia
  5. 5.Wadhwani Research Centre for BioengineeringIndian Institute of Technology BombayMumbaiIndia

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