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Journal of Applied Phycology

, Volume 28, Issue 4, pp 2139–2146 | Cite as

Tetraselmis suecica culture for CO2 bioremediation of untreated flue gas from a coal-fired power station

  • N. R. MoheimaniEmail author
Article

Abstract

The accumulation of atmospheric CO2, primarily due to combustion of fossil fuels, has been implicated in potential global climate change. The high rate of CO2 bioremediation by microalgae has emerged as a favourable method for reducing coal-fired power plant emissions. However, coal-fired power station flue gas contains other chemicals such as SOx which can inhibit microalgal growth. In the current study, the effect of untreated flue gas as a source of inorganic carbon on the growth of Tetraselmis in a 1000 L industrial-scale split-cylinder internal-loop airlift photobioreactor was examined. The culture medium was recycled after each harvest. Tetraselmis suecica grew very well in this airlift photobioreactor during the 7-month experiment using recycled medium from an electroflocculation harvesting unit. Increased medium SO4 2− concentration as high as 870 mg SO4 2− L−1 due to flue gas addition and media recycling had no negative effect on the overall growth and productivity of this alga. The potential organic biomass productivity and carbon sequestration using an industrial-scale airlift PBR at International Power Hazelwood, Gippsland, Victoria, Australia, are 178.9 ± 30 mg L−1 day−1 and 89.15 ± 20 mg ‘C’ L−1 day−1, respectively. This study clearly indicates the potential of growing Tetraselmis on untreated flue gas and using recycled medium for the purpose of biofuel and CO2 bioremediation.

Keywords

Airlift photobioreactor Recycled medium Microalgae Chlorophyta SOx Productivity Biofuel 

Notes

Acknowledgments

This study was solely funded by Victor Smorgon Group and with collaboration with the IPH. I would especially like to thank Mr Peter Edwards and Mr Jonathon Green of the Victor Smorgon Group for their unlimited support. I also greatly appreciate the unlimited support received from Tony Innocenzi, Chris Barfoot and Peter Massey from IPH. Especial thanks to Chris Barfoot for his assistance with the outdoor cultivation and laboratory analytical measurements. Also thanks to Sam Muresan, Dale Newing and Peter Gestos for managing the outdoor airlift photobioreactor. Also thanks to the colleagues from BioMax for their unlimited support in setting up and conducting these experiments.

References

  1. Agrawal M, Singh-Deepak S (2003) Physiological and biochemical responses of two cultures of wheat to elevated levels of CO2 and SO2 singly and in combination. Environ Pollut 121:189–197CrossRefPubMedGoogle Scholar
  2. APHA-AWWA-WEF (2012) 4500-SO4 2− E. turbidimetric method, 22nd edn. In: Rice EW, Baird RB, Eaton AD, Clesceri LS (eds) Standard methods for the examination of water and wastewater. American Public Health Association, Washington, DC, pp 4.190–4.191Google Scholar
  3. Baker LA, Urban NR, Brezonik PL, Sherman LA (1989) Sulfur cycling in a seepage lake. In: Saltzman E, Cooper W (eds) Biogenic sulphur in the environment. American Chemical Society, Washington, DC, pp 79–100CrossRefGoogle Scholar
  4. Beardall J, Raven JA (2013) Limits to phototrophic growth in dense culture: CO2 supply and light. In: Borowitzka MA, Moheimani NR (eds) Algae for biofuels and energy. Springer, Dordrecht, pp 91–97CrossRefGoogle Scholar
  5. Benemann JR (2013) Microalgae for biofuels and animal feeds. Energies 6:5869–5886CrossRefGoogle Scholar
  6. Benemann JR, Oswald WJ (1996) Systems and economic analysis of microalgae ponds for conversion of CO2 to biomass. Pittsburgh Energy Technology Center. Final report to the Department of Energy, Pittsburgh, p 201CrossRefGoogle Scholar
  7. Benemann JR, Goebel RP, Weismann JC, Augenstein DC (1982) Microalgae as a source of liquid fuels. Final technical report to US Department of Energy. US Department of Energy, Washington DCCrossRefGoogle Scholar
  8. Borowitzka MA (1997) Algae for aquaculture: opportunities and constraints. J Appl Phycol 9:393–401CrossRefGoogle Scholar
  9. Borowitzka MA (2013) High-value products from microalgae—their development and commercialisation. J Appl Phycol 25:743–756CrossRefGoogle Scholar
  10. Borowitzka MA, Larkum AWD (1976) Calcification in the green alga Halimeda II. The exchange of Ca2+ and the occurrence of age gradients in the calcification and photosynthesis. J Exp Bot 27:864–878CrossRefGoogle Scholar
  11. Borowitzka MA, Moheimani NR (2013a) Open pond culture systems. In: Borowitzka MA, Moheimani NR (eds) Algae for biofuels and energy. Springer, Dordrecht, pp 133–152CrossRefGoogle Scholar
  12. Borowitzka MA, Moheimani NR (2013b) Sustainable biofuels from algae. Mitig Adapt Strateg Glob Chang 18:13–25CrossRefGoogle Scholar
  13. Chiu SY, Kao CY, Chen CH, Kuan TC, Ong SC, Lin CS (2008) Reduction of CO2 by a high-density culture of Chlorella sp. in a semicontinuous photobioreactor. Bioresour Technol 99:3389–3396CrossRefPubMedGoogle Scholar
  14. Chiu SY, Kao CY, Tsai MT, Ong SC, Chen CH, Lin CS (2009) Lipid accumulation and CO2 utilization of Nannochloropsis oculata in response to CO2 aeration. Bioresour Technol 100:833–838CrossRefPubMedGoogle Scholar
  15. Cho S, Luong TT, Lee D, Oh YK, Lee T (2011) Reuse of effluent water from a municipal wastewater treatment plant in microalgae cultivation for biofuel production. Bioresour Technol 102:8639–8645CrossRefPubMedGoogle Scholar
  16. Cosgrove J, Borowitzka MA (2006) Applying pulse amplitude modulation (PAM) fluorometry to microalgae suspensions: stirring potentially impacts fluorescence. Photosynth Res 88:343–350CrossRefPubMedGoogle Scholar
  17. Cox PM, Betts RA, Jones CD, Spall SA, Totterdell IJ (2000) Acceleration of global warming due to carbon-cycle feedbacks in a coupled climate model. Nature 408:184–187CrossRefPubMedGoogle Scholar
  18. Eustance E, Wray JT, Badvipour S, Sommerfeld MR (2015) The effects of cultivation depth, areal density, and nutrient level on lipid accumulation of Scenedesmus acutus in outdoor raceway ponds. J Appl Phycol. doi: 10.1007/s10811-015-0709-z Google Scholar
  19. Fernández FGA, González-López CV, Sevilla JF, Grima EM (2012) Conversion of CO2 into biomass by microalgae: how realistic a contribution may it be to significant CO2 removal? Appl Microbiol Biotechnol 96:577–586CrossRefGoogle Scholar
  20. Fon Sing S, Borowitzka MA (2015) Isolation and screening of euryhaline Tetraselmis spp. suitable for large-scale outdoor culture in hypersaline media for biofuels. J Appl Phycol. doi: 10.1007/s10811-015-0560-2 Google Scholar
  21. Fon Sing S, Isdepsky A, Borowitzka MA, Lewis DM (2014) Pilot-scale continuous recycling of growth medium for the mass culture of a halotolerant Tetraselmis sp. in raceway ponds under increasing salinity: a novel protocol for commercial microalgal biomass production. Bioresour Technol 161:47–54CrossRefPubMedGoogle Scholar
  22. Grobbelaar J (1994) Turbulence in mass algal cultures and the role of light/dark fluctuations. J Appl Phycol 6:331–335CrossRefGoogle Scholar
  23. Hooper B, Innocenzi T, Dugan C (2009) The Hazelwood/H3 capture demonstration projects. In: International Pittsburgh Coal Conference Pittsburgh. PA. Curran Associates Inc, New YorkGoogle Scholar
  24. Isdepsky A (2015) Saline microalgae for biofuels: Outdoor culture from small-scale to pilot scale (Doctoral dissertation, Murdoch University)Google Scholar
  25. Lewis ER, Wallace DWR (1998) Program developed for CO2 system calculations. BNL-61827 informal. Oak Ridge National Laboratory, Oak RidgeCrossRefGoogle Scholar
  26. Maeda K, Owada M, Kimura N, Omata K, Karube I (1995) CO2 fixation from the flue gas on coal-fired thermal power plant microalgae. Energy Convers Manag 36:717–720CrossRefGoogle Scholar
  27. Moheimani NR (2013a) Inorganic carbon and pH effect on growth and lipid productivity of Tetraselmis suecica and Chlorella sp (Chlorophyta) grown outdoors in bag photobioreactors. J Appl Phycol 25:387–398CrossRefGoogle Scholar
  28. Moheimani NR (2013b) Long-term outdoor growth and lipid productivity of Tetraselmis suecica, Dunaliella tertiolecta and Chlorella sp (Chlorophyta) in bag photobioreactors. J Appl Phycol 25:167–176CrossRefGoogle Scholar
  29. Moheimani NR, Borowitzka M (2006) The long-term culture of the coccolithophore Pleurochrysis carterae (Haptophyta) in outdoor raceway ponds. J Appl Phycol 18:703–712CrossRefGoogle Scholar
  30. Moheimani NR, Borowitzka MA (2011) Increased CO2 and the effect of pH on growth and calcification of Pleurochrysis carterae and Emiliania huxleyi (Haptophyta) in semicontinuous cultures. Appl Microbiol Biotechnol 90:1399–1407CrossRefPubMedGoogle Scholar
  31. Moheimani NR, Webb JP, Borowitzka MA (2012) Bioremediation and other potential applications of coccolithophorid algae: a review. Algal Res 1:120–133CrossRefGoogle Scholar
  32. Moheimani NR, Borowitzka MA, Isdepsky A, Fon Sing S (2013) Standard methods for measuring growth of algae and their composition. In: Borowitzka MA, Moheimani NR (eds) Algae for biofuels and energy. Springer, Dordrecht, pp 265–284CrossRefGoogle Scholar
  33. Negoro M, Shioji N, Miyamoto K, Miura Y (1991) Growth of microalgae in high CO2 gas and effects of SOx and NOx. Appl Biochem Biotechnol 28–9:877–886CrossRefGoogle Scholar
  34. Negoro M, Shioji N, Ikuta Y, Makita T, Uchiumi M (1992) Growth characteristics of microalgae in high-concentration CO2 gas effects of culture medium trace components, and impurities thereon. Appl Biochem Biotechnol 34–5:681–692CrossRefGoogle Scholar
  35. Negoro M, Hamasaki A, Ikuta Y, Makita T, Hirayama K, Suzuki S (1993) Carbon dioxide fixation by microalgae photosynthesis using actual flue gas discharged from a boiler. Appl Biochem Biotechnol 39:643–653CrossRefGoogle Scholar
  36. Oron G, Shelef G, Levi A, Meydan A, Azov Y (1979) Algae/bacteria ratio in high rate ponds used for waste treatment. Appl Environ Microbiol 38:570–576PubMedPubMedCentralGoogle Scholar
  37. Raeesossadati MJ, Ahmadzadeh H, McHenry MP, Moheimani NR (2014) CO2 bioremediation by microalgae in photobioreactors: impacts of biomass and CO2 concentrations, light, and temperature. Algal Res 6:78–85CrossRefGoogle Scholar
  38. Romeo LM, Catalina D, Lisbona P, Lara Y, Martínez A (2011) Reduction of greenhouse gas emissions by integration of cement plants, power plants and CO2 capture systems. Greenhouse Gases: Sci Technol 1:72–82CrossRefGoogle Scholar
  39. Sahoo D, Elangbam G, Devi SS (2012) Using algae for carbon dioxide capture and biofuel production to combat climate change. Phykos 42:32–38Google Scholar
  40. Sridhar P, Namasivayam C, Prabhakaran G (1988) Algae flocculation in reservoir water. Biotech Bioeng 32:345–347CrossRefGoogle Scholar
  41. Strickland JDH, Parsons TR (1972) A practical handbook of seawater analysis, 2nd edn. Fish Res Board Can Bull 167:24–38Google Scholar
  42. Stumm W, Morgan JJ (1996) Aquatic chemistry: chemical equilibria and rates in natural waters. John Wiley and Sons, New YorkGoogle Scholar
  43. Vadiveloo A, Moheimani NR, Alghamedi R, Cosgrove JJ, Alameh K, Parlevliet D (2015) Sustainable cultivation of microalgae by an insulated glazed glass plate photobioreactor. Biotechnol J. doi: 10.1002/biot.201500358 PubMedGoogle Scholar
  44. Wijffels RH, Barbosa MJ (2010) An outlook on microalgal biofuels. Science 329:796–799CrossRefPubMedGoogle Scholar
  45. Wodzinski RS, Labeda DP, Alexander M (1978) Effects of low concentrations of bisulfate–sulfite and nitrite on microorganisms. Appl Environ Microbiol 35:718–723PubMedPubMedCentralGoogle Scholar
  46. Yang S, Wang J, Cong W, Cai Z, Ouyang F (2004) Effects of bisulfite and sulfite on the microalga Botryococcus braunii. Enzym Microb Technol 35:46–50CrossRefGoogle Scholar
  47. Yang J, Xu M, Zhang X, Hu Q, Sommerfeld M, Chen Y (2011) Life-cycle analysis on biodiesel production from microalgae: water footprint and nutrients balance. Bioresour Technol 102:159–165CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  1. 1.Algae R&D Centre, School of Veterinary and Life SciencesMurdoch UniversityMurdochAustralia

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