Anaerobic digestates from vinasse promote growth and lipid enrichment in Neochloris oleoabundans cultures
- 608 Downloads
Neochloris oleoabundans (=Ettlia oleoabundans) is a green microalga that has great potential for the production of biodiesel. To achieve economically viable processes for the production of biodiesel from microalgae, the use of wastewater is highly recommended. However, there are no reports on the cultivation of N. oleoabundans utilizing anaerobic digestates of vinasse or stillage, which is a highly polluting wastewater from the alcohol industry. A first group of experiments was conducted, aiming to establish the optimal culture conditions of N. oleoabundans (UTEX 1185) using anaerobic effluents of vinasse (AEV) in bubble columns incubated under controlled conditions. The highest culture density was obtained in a medium containing 6 % of such effluents with a daily addition of sodium bicarbonate (1 g L−1). The total lipid content varied from 17.7 to 38.5 % for a range of 2 to 8 % of AEV with added sodium bicarbonate. A second group of experiments using 6 % AEV + sodium bicarbonate and flat plate photobioreactor-incubated outdoors was performed. An increase of 62 % in cell density compared to the value registered in Bold’s basal medium (BBM) was observed. Furthermore, a high ammonium–nitrogen removal (85.2 %) and a high flocculation efficiency (42 % after 30 min) indicate that dual-purpose systems aimed at producing high densities of lipid-enriched biomass of this green microalga are feasible. The uses of supplemental bicarbonate and organic waste as a source of nutrients are very important factors that contribute to reducing the cost of production.
KeywordsChlorophyceae Anaerobically digested vinasse Auto-flocculation Biodiesel production Flocculation efficiency Green microalgae and stillage
The authors acknowledge the financial support from the grant FOMIX VER-2009-C03-127097 provided by the State of Veracruz and the National Council of Science and Technology (CONACYT) and from the grant 152931 provided by the Ministry of Energy (SENER) and CONACYT, México. They also acknowledge the technical support from José Luis Domínguez Zavala.
- APHA, American Public Health Association (1998) Methods for biomass production. In: Standard methods for the examination of water and wastewater. American Public Health Association, BaltimoreGoogle Scholar
- Elvira N, Ruíz-Marín A, Canedo-López Y (2013) Effect of nitrogen content and CO2 consumption rate by adding sodium carbonate in the lipid content of Chlorella vulgaris and Neochloris oleoabundans. Int J Environ Prot 3:13–19Google Scholar
- Joy EF, Barnard J (1975) Commercial acids and bases. In: Welcher FJ (ed) Standard methods of chemical analysis. Robert E. Krieger Publishing New York, pp 534–629Google Scholar
- Nguyen TDP, Frappart M, Jaouen P, Pruvost J, Bourseau P (2014) Harvesting Chlorella vulgaris by natural increase in pH: effect of medium composition. Environ Technol 35:1378–1388Google Scholar
- Olguín EJ, Mercado G, Hernández ME (2011) La contaminación del agua. In: Cruz A (ed) La biodiversidad en Veracruz: estudio de estado, volumen I, Comisión Nacional para el Conocimiento y Uso de la Biodiversidad, Gobierno del Estado de Veracruz. Universidad Veracruzana, Instituto de Ecología, México, pp 369–380Google Scholar
- Olguín EJ (2013) Plant and microalgae biomass for the production of biofuels and restoration of polluted environments within a biorefinery. Invited Lecture at the 2013 World Biotechnology Congress. Boston, MA. USA Abstract Book:18Google Scholar
- Pruvost J, Van Vooren G, Le Gouic B, Couzinet-Mossion A, Legrand J (2011) Systematic investigation of biomass and lipid productivity by microalgae in photobioreactors for biodiesel application. Bioresour Technol 102:150–158Google Scholar
- Robles-Pliego, M., Olguín, E.J., Hernández-Landa J., González-Portela, R.E., Sánchez-Galván, G., Cuervo-López F.M. Dual purpose system for the treatment of water from a polluted river and the production of Pistia stratiotes biomass within a biorefinery. (Accepted in CLEAN – Soil, Air & Water)Google Scholar