Journal of Applied Phycology

, Volume 27, Issue 4, pp 1415–1423 | Cite as

Effect of nitrogen source and acclimatization on specific growth rates of microalgae determined by a high-throughput in vivo microplate autofluorescence method

  • Michael Podevin
  • Davide De Francisci
  • Susan L. Holdt
  • Irini AngelidakiEmail author


Specific growth rates (SGR) of freshwater algae species (Chlorella vulgaris, Auxenochlorella protothecoides, and Chlorella sorokiniana) and the marine species Nannochloropsis oculata on various nitrogen sources (ammonium carbonate, ammonium chloride, sodium nitrate, and urea) could be determined by in vivo chlorophyll-a autofluorescence. These preferences could be determined before large pH changes occurred in the media, with no significant difference (P > 0.05) between buffered and non-buffered media. In all algal species, acclimatization was observed with no significant difference (P > 0.05) between SGRs of the second and third cultivations. ANOVA of SGRs in the acclimatized second and third cultivations revealed preferences for nitrogen sources among most of the algae; C. vulgaris preferred sodium nitrate over other nitrogen sources, A. protothecoides adapted to urea after no growth in the first cultivation, and the SGRs of N. oculata showed an aversion for sodium nitrate over other nitrogen sources (P < 0.05).


Adaptation Auxenochlorella Chlorella Nannochloropsis pH changes Industrial wastewater 



This work was funded by the European Commission (EC) Economically and Ecologically Efficient Water Management in the European Chemical Industry (E4Water) project (grant agreement no.: 280756).


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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Michael Podevin
    • 1
  • Davide De Francisci
    • 1
  • Susan L. Holdt
    • 1
  • Irini Angelidaki
    • 1
    Email author
  1. 1.Department of Environmental EngineeringTechnical University of DenmarkKgs. LyngbyDenmark

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