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Journal of Applied Phycology

, Volume 27, Issue 4, pp 1725–1735 | Cite as

Phylogeographic assessment of panmictic Monostroma species from Kuroshio Coast, Japan, reveals sympatric speciation

  • Felix Bast
  • Satoshi Kubota
  • Kazuo Okuda
Article

Abstract

The marine algae genus Monostroma (Thuret) consists of some of the common intertidal green seaweeds in the world. However, reports on its interbreeding potential between geographical radiations or its phylogeography are nonexistent. Here, we show that warmwater Monostroma in SW Japan, previously thought to be composed of two morphological species, viz., Monostroma latissimum and Monostroma nitidum, is indeed a single biological species, as revealed by mating tests. Using morphometry and DNA barcoding by primary and secondary structure analyses of highly variable first internal transcribed spacer (nrDNA ITS1) and more evolutionarily conserved gene encoding small subunit ribosome (nrDNA 18S), we conclude that isolates of Monostroma latissimum-nitidum complex represent an emergence of sympatric speciation. Our molecular phylogenetic analyses suggest that this alga originated in Ise Bay and was deliberately introduced elsewhere in Japan for cultivation purposes. Our phylogenetic analyses also suggest that the character state of the ability to interbreed is more evolutionarily conserved than either ontological or morphological differences in this alga. This is the first report of such an extensive intraspecific sequence divergence in any of the panmictic eukaryotes.

Keywords

Biological species DNA barcoding Kuroshio Morphometry nrDNA 18S nrDNA ITS1 Phylogeny Phylogeography Sympatric speciation 

Notes

Acknowledgments

This study was supported in part by Monbukagakusho Ph.D. fellowship from MEXT, Japanese Government, nominated by Ministry of Human Resources Development, Government of India, awarded to FB. FB also thank Japan Railways for contributing sampling expeditions undertaken in this report via discounted tickets (Seishun Jyuhachi Kippu). The following people kindly assisted us during the acquisition of samples: Masafumi Iima, Mine Ichiro, Miyuki Maegawa, Norishige Yotsukura, Ryuta Terada, Christine A Maggs, and Frédéric Mineur. MP Ajaikumar contributed his time and expertise in reading this manuscript that led to some improvisations.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.Graduate School of Kuroshio ScienceKochi UniversityKochiJapan
  2. 2.Centre for BiosciencesCentral University of PunjabBathindaIndia

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