Journal of Applied Phycology

, Volume 27, Issue 2, pp 685–696 | Cite as

Comparative analysis of triacylglycerols from different Stichococcus strains by RP-HPLC/APCI-MS and chiral HPLC

  • Tomáš ŘezankaEmail author
  • Linda Nedbalová
  • Karel Sigler


The main analytical benefit of this study is the development of methods enabling a rapid determination of triacylglycerols (TAGs) in different strains of the green alga Stichococcus bacillaris and detailed identification and quantification of a complex mixture of natural TAGs by reversed phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (RP-HPLC/APCI-MS) and chiral HPLC. Neutral ion scan can readily identify, both qualitatively and semiquantitatively, triacylglycerols containing 16:2 and 16:3 fatty acids (FAs) in the molecule. Since more detailed studies in this respect have been stymied by the shortage of 16:2 and 16:3 FAs, we decided to study the alga Stichococcus as a potential new biotechnological source of C16 polyunsaturated fatty acids (PUFAs). We conclude that the genus Stichococcus is a major producer of C16 PUFAs mostly contained in TAGs. Further, the ratio of regioisomers and enantiomers of TAGs depends on the content of fatty acids; with lowering temperature, the proportion of the achiral TAG increases, and so does the enantiomer ratio from 1:1. Dienoic FAs preferentially esterify position sn-3, whereas in trienoic acids, it is position sn-1.


Green alga Stichococcus bacillaris Triacylglycerols APCI Neutral loss scan RP-HPLC/APCI-MS Chiral LC 



The research was supported by the project GACR P503/11/0215 and by the Institutional Internal Project RVO61388971.

Supplementary material

10811_2014_354_MOESM1_ESM.pdf (545 kb)
ESM 1 (PDF 545 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Tomáš Řezanka
    • 1
    Email author
  • Linda Nedbalová
    • 2
  • Karel Sigler
    • 1
  1. 1.Institute of MicrobiologyAcademy of Sciences of the Czech RepublicPragueCzech Republic
  2. 2.Department of Ecology, Faculty of ScienceCharles University in PraguePrague 2Czech Republic

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