Journal of Applied Phycology

, Volume 26, Issue 2, pp 1287–1300 | Cite as

Molecular evidence confirms the parasite Congracilaria babae (Gracilariaceae, Rhodophyta) from Malaysia

  • Poh-Kheng Ng
  • Phaik-Eem Lim
  • Aki Kato
  • Siew-Moi Phang
Article

Abstract

A phylogenetic study of two parasites found on the common host species Gracilaria salicornia from Japan and Malaysia based on nuclear, plastid, and mitochondrial molecular markers was conducted. The Japanese and Malaysian parasites were placed in the same cluster in the nuclear phylogenies inferred from the LSU rRNA gene and ITS region, supporting the monophyly of these parasites despite the slight anatomical variations observed. In addition to the molecular analyses, shared morphological and reproductive features including the lack of rhizoids, similar coloration to hosts, and the presence of deep spermatangial conceptacles supported the inclusion of both the Japanese and Malaysian parasites in Congracilaria babae. Phylogenetic analyses based on ITS sequences indicated that C. babae most likely evolved directly from its current host species, G. salicornia. C. babae was shown to be a member of Gracilaria sensu stricto along with its host. C. babae growing on G. salicornia is characterized by having plastid rbcL and mitochondrial cox1 gene sequences identical to those of its host, whereas the nuclear LSU rRNA gene and ITS region sequences are different from those of its host.

Keywords

Red algal parasites Congracilaria babae Gracilaria salicornia ITS region LSU rRNA gene rbccox

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Poh-Kheng Ng
    • 1
    • 2
  • Phaik-Eem Lim
    • 1
    • 2
  • Aki Kato
    • 3
  • Siew-Moi Phang
    • 1
    • 2
  1. 1.Institute of Biological Sciences, Faculty of ScienceUniversity of MalayaKuala LumpurMalaysia
  2. 2.Institute of Ocean and Earth SciencesUniversity of MalayaKuala LumpurMalaysia
  3. 3.Takehara Fisheries Research Station, Center for Education and Research of Field Science, Hiroshima UniversityTakeharaJapan

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