Microsatellite markers for the palaeo-temperature indicator Pentapharsodinium dalei (Dinophyceae)
- 218 Downloads
Pentapharsodinium dalei is a widely distributed cold-water dinoflagellate, which is used in palaeoecology as an indicator of relatively warmer conditions in polar and sub-polar regions. This species has been proposed to be one of the first indicators of global warming at high latitudes. We developed the first microsatellite markers for P. dalei to facilitate the study of spatial and temporal population genetic changes. Single cysts were isolated from surface sediments in Koljö Fjord, Sweden. After cyst germination, single vegetative cells were isolated for establishing monoclonal cultures. Six dinucleotide polymorphic microsatellite markers were developed as multiplex polymerase chain reactions and were genotyped in 32 strains. The number of alleles per locus varied between 4 and 12, and the estimated gene diversity varied from 0.588 to 0.891. The haploid state of the vegetative cells was confirmed. The six selected microsatellites will be useful to explore population dynamics in P. dalei from contemporary planktonic and revived benthic samples to enable, for example, detailed studies into the evolutionary consequences of anthropogenic and climate-driven habitat changes.
KeywordsDinoflagellate Global change Microsatellites Multiplex PCR Pentapharsodinium dalei Population genetics Sediment Temperature
This study was part of the Danish Research Council project 2111-04-0011. SR holds a postdoctoral grant from the Carlsberg Foundation, Denmark (no. 2011_01_0337).
- Dale B (1996) Dinoflagellate cyst ecology: modelling and geological applications. In: Jansonius J, McGregor DC (eds) Palynology: principles and applications. The American Association of Stratigraphic Palynologists Foundation, Salt Lake City, pp 1249–1276Google Scholar
- Doyle JJ, Doyle JL (1990) A rapid total DNA preparation procedure for fresh plant tissue. Focus 12:13–15Google Scholar
- Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinformat Online 1:47–50Google Scholar
- Larsen NH, Moestrup Ø, Pedersen PM (1994) Catalogue 1994. Scandinavian Culture Centre for Algae & Protozoa. Department of Phycology. Botanical Institute. University of Copenhagen. http://www.sccap.dk/media/marine/1.asp
- Masseret E, Grzebyk D, Nagai S, Genovesi B, Lasserre B, Laabir M, Collos Y, Vaquer A, Berrebi P (2009) Unexpected genetic diversity among and within populations of the toxic dinoflagellate Alexandrium catenella as revealed by nuclear microsatellite markers. Appl Environ Microbiol 75:2037–2045PubMedCentralPubMedCrossRefGoogle Scholar
- Nei M (1987) Molecular evolutionary genetics, 1st edn. Columbia University, New YorkGoogle Scholar
- Pfiester LA, Anderson DM (1987) Dinoflagellate reproduction. In: Taylor FJR (ed) The biology of dinoflagellates. Blackwell, Oxford, pp 611–648Google Scholar
- Rochon A, Vernal A, Turon J, Matthießen J, Head M (1999) Distribution of recent dinoflagellate cysts in surface sediments from the North Atlantic Ocean and adjacent seas in relation to sea-surface parameters. Am Ass Stratigr Palynol Contrib Ser 35:1–146Google Scholar
- Sperisen C, Gugerli F, Bucher U, Mátyás G (2000) Comparison of two rapid DNA extraction protocols for gymnosperm for application in population genetic and phylogenetic studies. Forest Gen 7:133–136Google Scholar