Journal of Applied Phycology

, Volume 26, Issue 1, pp 299–309 | Cite as

Organic and inorganic nitrogen utilization by nitrogen-stressed cyanobacteria during bloom conditions

Article

Abstract

Cyanobacterial blooms often occur in lakes that have high phosphorus (P) and low nitrogen (N) concentrations, and the growth rate of the blooms is often constrained by N. For these reasons, many researchers have suggested that regulation of both P and N is required to control eutrophication. However, because N occurs in many bioavailable forms, regulation of a particular form may be beneficial rather than regulation of all N forms. To address how N-stressed cyanobacteria respond to various N inputs, N enrichment experiments (nitrate, ammonium, urea, and alanine) were performed during N-limited cyanobacterial blooms in Maumee and Sandusky Bays of Lake Erie and in Grand Lake St. Marys (GLSM). Bioavailable N (nitrate, urea, and ammonium) concentrations were also determined. Microcystis aeruginosa dominated the Maumee Bay bloom, where the highest growth rates were in response to ammonium additions, and lowest growth rates were in response to nitrate. Urea and the amino acid alanine resulted in intermediate growth rates. Planktothrix agardhii dominated the Sandusky Bay and GLSM blooms, where nitrate, ammonium, and urea addition resulted in similar growth rates. Additions of alanine did not stimulate growth of the Planktothrix blooms. Incubations using stable isotope 15N showed the cyanobacteria had a preference for ammonium, but the other forms were also assimilated in the presence of ammonium. These results show that cyanobacterial blooms will assimilate multiple forms of N to support growth. Thus, if lake managers do decide that N abatement is necessary, then all forms of bioavailable N need to be constrained.

Keywords

Cyanobacteria Eutrophication Microcystis Nitrogen Planktothrix 

Notes

Acknowledgments

We thank Courtney Mobilian for assistance with the experiments, Dr. Sasmita Mishra and the Heckathorn laboratory for use of the spectrophotometer for urea analysis. Drs. Doug Kane, Scott Heckathorn, Von Sigler, Darren Bade, and Mike McKay provided a friendly review on an earlier draft. We thank two anonymous reviewers who helped improve this manuscript. This project was funded by Lake Erie Protection Fund small grant program award # SG 406–11, the National Science Foundation grant no. 1039043.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Department of Environmental Sciences and Lake Erie CenterUniversity of ToledoOregonUSA
  2. 2.F.T. Stone LaboratoryOhio State University and Ohio Sea GrantPut-In-BayUSA

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