Journal of Applied Phycology

, Volume 26, Issue 1, pp 629–634 | Cite as

Effect of the commercial extract of the brown alga Ascophyllum nodosum Mont. on Kappaphycus alvarezii (Doty) Doty ex P.C. Silva in situ submitted to lethal temperatures

  • Rafael Rodrigues Loureiro
  • Renata Perpetuo Reis
  • Rafael Guedes Marroig
Article

Abstract

Kappaphycus alvarezii is being introduced in several countries and in some of them there is a need to adapt this cultivation to periods with lethal temperatures, such as the 16–18 °C that occurs in the winter in southern Brazil. Moreover, there is the need to maintain the seedlings during this lethal temperature period. Considering the promising results obtained with the commercial powder extract of Ascophyllum nodosum (Acadian marine plant extract powder—AMPEP) treatment in the cultivation of K. alvarezii in vitro and in the sea allowing more resistance to epiphytes and increasing the growth rate and carrageenan yield, it was hypothesized that seedlings previously subjected to an AMPEP treatment could be more resilient to lethal temperatures. The daily growth rate and carrageenan yield and gel quality (gel strength and gel viscosity) of K. alvarezii in vitro previously treated with AMPEP were analyzed under temperature stress. The daily growth rates and the gel strengths of the AMPEP-treated samples were increased. In spite of the lower carrageenan yield and lower gel viscosity, the values were within the ones accepted by the carrageenan industry. Thus, the treatment of the seedlings of the K. alvarezii with AMPEP solution can be used as an alternative to lower temperature effects on crops as a preventive action for the cultivation of the seedlings in tanks and in the sea in periods of low temperatures at sea.

Keywords

Aquaculture Biotechnology Improvement Lethal temperatures Resilience 

References

  1. Barros MP, Necchi O Jr, Colepicolo P, Pedersén M (2006) Kinetic study of the plastoquinone pool availability correlated with H2O2 release in seawater and antioxidant responses in the red alga Kappaphycus alvarezii exposed to single or combined high light, chilling and chemical stresses. Biochim Biophys Acta 1757:1520–1528PubMedCrossRefGoogle Scholar
  2. Bindu MS, Levine IA (2011) The commercial red seaweed Kappaphycus alvarezii—an overview on farming and environment. J Appl Phycol 23:789–796CrossRefGoogle Scholar
  3. Bixler HJ, Porse H (2011) A decade of change in the seaweed hydrocolloids industry. J Appl Phycol 23:321–335CrossRefGoogle Scholar
  4. Borlongan IAG, Tibudos KR, Yunque DAT, Hurtado AQ, Critchley AT (2011) Impact of AMPEP on the growth and occurrence of epiphytic Neosiphonia infestation on two varieties of commercially cultivated Kappaphycus alvarezii grown at different depths in the Philippines. J Appl Phycol 23:615–621CrossRefGoogle Scholar
  5. Craigie JS (1990) Cell walls. In: Cole KM, Sheath RG (eds) Biology of the Red Algae. Cambridge University Press, Cambridge, pp 221–225Google Scholar
  6. Craigie JS, Wen ZC (1984) Effects of temperature and tissue age on gel stregth and composition of agar from Gracilaria tikhaviae (Rhodophyta). Can J Bot 62:1665–1670CrossRefGoogle Scholar
  7. Dawes CJ, Orduña-Rojas J, Robledo D (1999) Response of the tropical red seaweed Gracilaria cornea to temperature, salinity and irradiance. J Appl Phycol 10:419-425Google Scholar
  8. Góes HG, Reis RP (2011) An initial comparison of tubular netting versus tietie methods of cultivation for Kappaphycus alvarezii (Rhodophyta, Solieriaceae) on the south coast of Rio de Janeiro State, Brazil. J Appl Phycol 23:607–613CrossRefGoogle Scholar
  9. Góes HG, Reis RP (2012) Temporal variation of the growth, carrageenan yield and quality of Kappaphycus alvarezii (Rhodophyta, Gigartinales) cultivated at Sepetiba Bay, southeastern Brazilian coast. J Appl Phycol 24:173–180CrossRefGoogle Scholar
  10. Hayashi L, Oliveira EC, Bleicher-Lhonneur G, Boulenguer P, Pereira RTL, von Seckendorff R, Shimoda VT, Leflamand A, Vallée P, Critchley AT (2007) The effects of selected cultivation conditions on the carrageenan characteristics of Kappaphycus alvarezii (Rhodophyta, Solieriaceae) in Ubatuba Bay, São Paulo, Brazil. J Appl Phycol 19:505–511CrossRefGoogle Scholar
  11. Hayashi L, Santos AA, Faria GSM, Nunes BG, Souza MS, Fonseca ALD, Barreto PLM, Oliveira EC, Bouzon ZL (2011) Kappaphycus alvarezii (Rhodophyta, Areschougiaceae) cultivated in subtropical waters in Southern Brazil. J Appl Phycol 23:337–343CrossRefGoogle Scholar
  12. Hung LD, Hori K, Nang HQ, Kha T, Hoa LT (2009) Seasonal changes in growth rate, carrageenan yield and lectin content in the red alga Kappaphycus alvarezii cultivated in Camranh Bay, Vietnam. J Appl Phycol 21:265–272CrossRefGoogle Scholar
  13. Hurtado AQ, Yunque DA, Tibudos K, Critchley AT (2009) Use of Acadian marine plant extract powder from Ascophyllum nodosum in tissue culture of Kappaphycus varieties. J Appl Phycol 21:633–639CrossRefGoogle Scholar
  14. Jayaraj J, Wan A, Rahman M, Punja ZK (2008) Seaweed extract reduces foliar fungal diseases on carrots. Crop Prot 27:1360–1366CrossRefGoogle Scholar
  15. Jayaraman J, Norrie J, Punja ZK (2011) Commercial extract from the brown seaweed Ascophyllum nodosum reduces diseases in greenhouse cucumber. J Appl Phycol 23:353–361CrossRefGoogle Scholar
  16. Khan W, Palanisamy R, Hankins SD, Critchley AT, Smith DL, Papadopoulos Y, Prithiviraj B (2008) Ascophyllum nodosum (L.) Le Jolis extract improves root nodulation in Alfalfa. Can J Plant Sci 88:728–728Google Scholar
  17. Khan W, Rayirath UP, Subramanian UP, Jitesh MN, Rayorath P, Hodges DM, Critchley AT, Craigie JS, Norrie J, Prithiviraj B (2009) Seaweed extracts as biostimulants of plant growth and development. J Plant Growth Reg 28:386–39CrossRefGoogle Scholar
  18. Knutsen SH, Sletmoen M, Kristensen T, Barbeyron T, Kloareg B, Potin P (2001) A rapid method for the separation and analysis of carrageenan oligosaccharides released by iota and kappa-carrageenase. Carbohydrates 331:101–106CrossRefGoogle Scholar
  19. Loureiro RR, Reis RP, Berrogain FD, Critchley AT (2012) Extract powder from the brown alga Ascophyllum nodosum (Linnaeus) Le Jolis (AMPEP): a vaccine-like effect on Kappaphycus alvarezii (Doty) Doty ex P.C. Silva. J Appl Phycol 24:427–432CrossRefGoogle Scholar
  20. Loureiro RR, Reis RP, Critchley AT (2010) In vitro cultivation of three Kappaphycus alvarezii (Rhodophyta, Areschougiaceae) variants (green, red and brown) exposed to a commercial extract of the brown alga Ascophyllum nodosum (Fucaceae, Ochrophyta). J Appl Phycol 22:101–104CrossRefGoogle Scholar
  21. Mackinnon SL, Hiltz D, Ugarte R, Craft CA (2010) Improved methods of analysis for betaines in Ascophyllum nodosum and its commercial seaweed extracts. J Appl Phycol 22:489–494Google Scholar
  22. Mendonza WG, Montaño NE, Ganzon-Fortes ET, Villanueva RD (2002) Chemical and gelling profile of ice–ice infected carrageenan from Kappaphycus striatum (Schmitz) Doty sacol strain (Solieriaceae, Gigartinales, Rhodophyta). J Appl Phycol 14:409–418CrossRefGoogle Scholar
  23. Nair P, Kandasamy S, Zhang J, Ji X, Kirby C, Benkel B, Hodges MD, Critchley AT, Hiltz D, Prithiviraj B (2012) Transcriptional and metabolic analysis of Ascophyllum nodosum mediates freezing tolerance in Arabidopsis thaliana. BMC Genomics 13:643PubMedCentralPubMedCrossRefGoogle Scholar
  24. Ohno M, Largo DB, Ikumoto T (1994) Growth rate, carrageenan yield and gel properties of cultured kappa carrageenan producing red alga Kappaphycus alvarezii (Doty) Doty in the subtropical waters of Shikoku, Japan. J Appl Phycol 6:1–5CrossRefGoogle Scholar
  25. Parys S, Kehraus S, Pete R, Kupper FC, Glombitza KW, Konig GW (2009) Seasonal variation of polyphenolics in Ascophyllum nodosum (Phaeophyceae). Eur J Phycol 44:331–338CrossRefGoogle Scholar
  26. Rayirath P, Benkel B, Hodges MD, Allan-Wojitas P, MacKinnon S, Critchley AT, Prithiviraj B (2009) Lipophilic components of the brown seaweed, Ascophyllum nodosum, enhance freezing tolerance in Arabidopsis thaliana. Planta 230:135–147PubMedCrossRefGoogle Scholar
  27. Reitz SR, Trumble JT (1996) Effects of cytokinin-containing seaweed extract on Phaseolus lunatus L.: influence of nutrient availability and apex removal. Bot Mar 39:33–38CrossRefGoogle Scholar
  28. Rudolph AS, Crowe JH, Crowe LM (1986) Effects of three stabilizing agents—proline, betaine, and trehalose on membrane phospholipids. Arch Biochem Biophys 245:134–143PubMedCrossRefGoogle Scholar
  29. Yong YS, Yong WTL, Anton A (2013) Analysis of formulae for determination of seaweed growth rate. J Appl Phycol DOI. doi:10.1007/s10811-013-0022-7 Google Scholar
  30. Yoshiba Y, Kiyosue T, Nakashima K, Yamaguchi-Shinozaki K, Shinozaki K (1997) Regulation of levels of proline as an osmolyte in plants under water stress. Plant Cell Physiol 38:1095–1102PubMedCrossRefGoogle Scholar
  31. Yunque DAT, Tibudos KR, Hurtado AQ, Critchley AT (2011) Optimization of culture conditions for tissueculture production of young plantlets of carrageenophyte Kappaphycus. J Appl Phycol 23:433–438CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Rafael Rodrigues Loureiro
    • 1
  • Renata Perpetuo Reis
    • 1
  • Rafael Guedes Marroig
    • 1
  1. 1.Instituto de Pesquisas Jardim Botânico do Rio de JaneiroRio de JaneiroBrazil

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