Effect of the commercial extract of the brown alga Ascophyllum nodosum Mont. on Kappaphycus alvarezii (Doty) Doty ex P.C. Silva in situ submitted to lethal temperatures
- 284 Downloads
Kappaphycus alvarezii is being introduced in several countries and in some of them there is a need to adapt this cultivation to periods with lethal temperatures, such as the 16–18 °C that occurs in the winter in southern Brazil. Moreover, there is the need to maintain the seedlings during this lethal temperature period. Considering the promising results obtained with the commercial powder extract of Ascophyllum nodosum (Acadian marine plant extract powder—AMPEP) treatment in the cultivation of K. alvarezii in vitro and in the sea allowing more resistance to epiphytes and increasing the growth rate and carrageenan yield, it was hypothesized that seedlings previously subjected to an AMPEP treatment could be more resilient to lethal temperatures. The daily growth rate and carrageenan yield and gel quality (gel strength and gel viscosity) of K. alvarezii in vitro previously treated with AMPEP were analyzed under temperature stress. The daily growth rates and the gel strengths of the AMPEP-treated samples were increased. In spite of the lower carrageenan yield and lower gel viscosity, the values were within the ones accepted by the carrageenan industry. Thus, the treatment of the seedlings of the K. alvarezii with AMPEP solution can be used as an alternative to lower temperature effects on crops as a preventive action for the cultivation of the seedlings in tanks and in the sea in periods of low temperatures at sea.
KeywordsAquaculture Biotechnology Improvement Lethal temperatures Resilience
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) supported this research. The authors would like to thank Acadian Seaplants Limited for providing the AMPEP and Henrique Geromél de Góes for providing K. alvarezii samples.
- Barros MP, Necchi O Jr, Colepicolo P, Pedersén M (2006) Kinetic study of the plastoquinone pool availability correlated with H2O2 release in seawater and antioxidant responses in the red alga Kappaphycus alvarezii exposed to single or combined high light, chilling and chemical stresses. Biochim Biophys Acta 1757:1520–1528PubMedCrossRefGoogle Scholar
- Borlongan IAG, Tibudos KR, Yunque DAT, Hurtado AQ, Critchley AT (2011) Impact of AMPEP on the growth and occurrence of epiphytic Neosiphonia infestation on two varieties of commercially cultivated Kappaphycus alvarezii grown at different depths in the Philippines. J Appl Phycol 23:615–621CrossRefGoogle Scholar
- Craigie JS (1990) Cell walls. In: Cole KM, Sheath RG (eds) Biology of the Red Algae. Cambridge University Press, Cambridge, pp 221–225Google Scholar
- Dawes CJ, Orduña-Rojas J, Robledo D (1999) Response of the tropical red seaweed Gracilaria cornea to temperature, salinity and irradiance. J Appl Phycol 10:419-425Google Scholar
- Hayashi L, Oliveira EC, Bleicher-Lhonneur G, Boulenguer P, Pereira RTL, von Seckendorff R, Shimoda VT, Leflamand A, Vallée P, Critchley AT (2007) The effects of selected cultivation conditions on the carrageenan characteristics of Kappaphycus alvarezii (Rhodophyta, Solieriaceae) in Ubatuba Bay, São Paulo, Brazil. J Appl Phycol 19:505–511CrossRefGoogle Scholar
- Khan W, Palanisamy R, Hankins SD, Critchley AT, Smith DL, Papadopoulos Y, Prithiviraj B (2008) Ascophyllum nodosum (L.) Le Jolis extract improves root nodulation in Alfalfa. Can J Plant Sci 88:728–728Google Scholar
- Mackinnon SL, Hiltz D, Ugarte R, Craft CA (2010) Improved methods of analysis for betaines in Ascophyllum nodosum and its commercial seaweed extracts. J Appl Phycol 22:489–494Google Scholar